Abstract:
PURPOSE: A SNP marker for predicting fatty acid composition of a crossbreed pig is provided to predict fatty acid composition, especially for a composition of unsaturated fatty acid and saturated fatty acid, thereby utilizing the SNP marker for selection of healthy pig entity and high value pork production. CONSTITUTION: A polynucleotide or corresponding polynucleotide for predicting fatty acid composition of a crossbreed pig is selected from polynucleotide having nucleic acid sequence of sequence number 1. The polynucleotide or corresponding polynucleotide comprises 0 or more of successive nucleic acids which include 817th of the sequence number 1 or 14384th SNP related base. The crossbreed pig is a crossbreed between Korea native kind and Landrace. The crossbreed pig is F_2. A polynucleotide which specifically mix with the polynucleotide or the corresponding polynucleotide is provided.
Abstract:
본 발명은 돼지의 C2 유전자의 돌연변이 여부를 측정하는 제제를 포함하는, 돼지 혈청 내 칼슘 이온 농도를 예측하기 위한 마커 검출용 조성물, 상기 조성물을 포함하는 키트, 및 상기 마커를 이용하여 돼지 혈청 내 칼슘 이온 농도를 예측하는 방법에 관한 것으로서, 돼지 보체 요인 유전자인 C2 유전자 내에 품종 특이성 변이를 탐색하여, 혈청 내 Ca ++ 농도를 용이하게 예측하는 효과를 갖는다.
Abstract:
본 발명은 올리고뉴클레오타이드 연결 분석법을 이용한 KIT 유전자 복제수 변이의 정량 분석방법에 관한 것이다. 보다 구체적으로 본 발명은 올리고뉴클레오타이드 연결 분석법을 이용한 KIT 유전자 중복의 복제수 변이 분석방법, KIT 유전자 중복의 복제수 변이는 상기 올리고뉴클레오타이드 연결 분석법에 의해 측정하고, KIT 유전자의 스플라이싱 변이는 종래의 파이로시퀀싱 법에 의해 측정하는 것을 특징으로 하는 KIT 유전자 복제수 변이의 정량방법, 상기 방법에 의해 측정된 KIT 유전자 복제수 변이에 따라 유전자형을 분류하는 것을 특징으로 하는 돼지 모색 관련 KIT 유전자의 유전자형 분석방법에 관한 것이다. 본 발명의 방법은 종래의 방법에 비해 보다 더 정확하고 정밀하게 KIT 유전자 복제수 변이의 정량할 수 있으므로, 본 발명의 방법은 KIT 유전자 중복 상태의 탐지와 유전자형 분석에 매우 유용하다. KIT 유전자, 복제수 변이, 유전자형 분석
Abstract:
The present invention relates to a DNA marker for determining sex and, more specifically, to a DNA marker for determining sex of pocine meat. The present invention is a technique which facilitates construction of a back tracking system which constructs D/B by performing DNA tests using the DNA marker for determining sex, and finds parent individual using DNA test results of porkers. Also, the present invention is a technique usable for producing male and female customized domestic animals, or for preventing male and female transformed meat. The present invention performs both scientific verification method and crackdown method by applying phase-DNA examination method, thereby being able to be applied to construction of a traceability system.
Abstract:
PURPOSE: A marker for detecting swine hair color and a detection method are provided to develop a technique for removing a factor generating hair color. CONSTITUTION: A marker for detecting swine hair color contains SNP or copy number variation(CNV) of swine KIT gene having 123th base of sequence number 5 in which G is substituted with A and a mixture thereof. The pig is Landrace, Yorkshire, or Large White. A kit contains the marker. A method for detecting the swine hair color factor comprises: a step of preparing a nucleic acid sample from a subject; a step of identifying base sequence of SNP and CNV of swine KIT gene; and a step of determining high probability in a pig containing a SNP of swine KIT gene at 123th base of sequence number 5 is G/G without CNV of KIT gene.
Abstract:
PURPOSE: A composition for detecting marker for predicting serum calcium ion in Sus scrofa is provided to detect specificity variation in C2 gene. CONSTITUTION: A composition for detecting a marker for predicting serum calcium ion in Sus scrofa contains an agent for measuring missense mutation of Sus scrofa C2(complement component 2). A kit for detecting the marker for predicting the serum calcium ion Sus scrofa contains the composition. A method for predicting the serum calcium ion comprises: a step of extracting C2 gene DNA from Sus scrofa sample; a step of amplifying the DNA; and a step of performing pyrosequencing.
Abstract:
A quantitative analysis method for copy number variation of KIT gene is provided to detect duplication of KIT gene and analyze genotype by quantitating copy number variation of KIT gene using an oligonucleotide ligation assay. A quantitative analysis method for copy number variation of KIT gene comprises the following steps of: PCR-amplifying a DNA sample to be tested using a specific primer for L1MC1/L1ME1 regions; treating the amplified product with proteinase; and amplifying proteinase-treated product with a primer wherein a fluorescent material is spliced and measuring copy number variation of gene duplication(C/G) generated at a duplication breakpoint region associated with KIT loci. The DNA sample is isolated from swine blood or hair root.