公开(公告)号：KR100311891B1

公开(公告)日：2001-11-03

申请号：KR1019990043339

申请日：1999-10-08

Applicant: 한국과학기술연구원

Inventor： 김성호 ,  유연규 ,  한예선 ,  김상숙

IPC: C12N15/54 ,  C12N15/70

Abstract: 본발명은초고온미생물중 진정세균인아퀴펙스파이로필러스(Aquifex pyrophilus)로부터단리한강한내열성을갖는글루타메이트라세메이즈효소의유전자, 이로부터발현되는아미노산서열을갖는글루타메이트라세메이즈, 상기유전자를함유하는발현벡터로형질전환된대장균에서상기글루타메이트라세메이즈를제조하는방법에관한것이다. 보다구체적으로, 본발명은서열 3에나타낸염기서열을갖는유전자및 이로부터발현된아미노산서열을갖는내열성글루타메이트라세메이즈에관한것이다. 본발명에서단리된아퀴펙스파이로필러스의글루타메이트라세메이즈의유전자를포함하는발현벡터로형질전환된대장균에서생산된글루타메이트라세메이즈는기존의중온성세균에서단리된효소보다고온, pH 및유기용매등의조건하에서도높은안정성을갖기때문에의약품생산및 식품소재의생산등과같이산업적으로유용하게사용될수 있다.

公开(公告)号：KR1020010036363A

公开(公告)日：2001-05-07

申请号：KR1019990043339

申请日：1999-10-08

Applicant: 한국과학기술연구원

Inventor： 김성호 ,  유연규 ,  한예선 ,  김상숙

IPC: C12N15/54 ,  C12N15/70

CPC classification number: C12N9/10 ,  C12N15/70 ,  C12Y501/01003

Abstract: PURPOSE: Provided is a gene of heat-resistant glutamate racemase which show stability against high temperatures, pH, and organic solvent and is useful in manufacturing medicines and food. CONSTITUTION: Heat resistant glutamate racemase is manufactured by the steps of: isolating gene of glutamate racemase consisting of 762 bases from which 254 amino acids are synthesized, from Aquifex pyrophilus; transforming E.coli is with an expression vector pGR1 that contains the gene; culturing E.coli transformant and destroying transformant cell; and recovering heat resistant glutamate racemase from cell lysat by chromatography. The base sequence of glutamate racemase shows 26.6%, 36.1%, and 35.5% of similarity with E. coli, Bacillus subtilus, and Lactobacillus, respectively.

Abstract translation: 目的：提供耐高温，pH和有机溶剂稳定性的耐热谷氨酸消旋酶基因，可用于制造药物和食品。 构成：通过以下步骤制备耐热谷氨酸消旋酶：从Aquifex pyrophilus分离由合成了254个氨基酸的762个碱基组成的谷氨酸消旋酶基因; 转化大肠杆菌含有该基因的表达载体pGR1; 培养大肠杆菌转化体并破坏转化细胞; 并通过色谱从细胞裂解液回收耐热谷氨酸消旋酶。 谷氨酸消旋酶的碱基序列分别与大肠杆菌，枯草芽孢杆菌和乳杆菌分别具有26.6％，36.1％和35.5％的相似性。