公开(公告)号：KR100071318B1

公开(公告)日：1994-02-26

申请号：KR1019870006560

申请日：1987-06-27

Applicant: 한국과학기술연구원

Inventor： 한문희 ,  정태화 ,  김지영 ,  강성만 ,  김성완 ,  하현정 ,  나도선

IPC: C12N15/00

公开(公告)号：KR1019890003715B1

公开(公告)日：1989-09-30

申请号：KR1019870006560

申请日：1987-06-27

Applicant: 한국과학기술연구원

Inventor： 한문희 ,  정태화 ,  김지영 ,  강성만 ,  김성완 ,  하현정 ,  나도선

IPC: C12N15/00

Abstract: A fragment having -300 base pairs was obtained from a plasmid, PILM 7, cut by XbaI and PvuII. On the other hand, a fragment having - 4,500 base pairs was obtained from aplasmid, pNK2, treated with XbaI and PvuII. Both of the fragments were ligated and inserted into a proper strain to get pNKM 21. pNKM 21 was inserted into M 5248 strain to get KCTC 8258 P strain followed by culturing it to give a interleukin-2 contg. serine at 125-position instead of cystein.

Abstract translation: 从由XbaI和PvuII切割的质粒PILM7获得具有-300个碱基对的片段。 另一方面，从用XbaI和PvuII处理的质粒pNK2获得具有-4,500个碱基对的片段。 将两个片段连接并插入适当的菌株中以获得pNKM21。将pNKM 21插入M 5248菌株中以获得KCTC 8258P菌株，然后培养以产生白细胞介素-2对照。 丝氨酸位于125位，而不是半胱氨酸。

公开(公告)号：KR100020571B1

公开(公告)日：1986-01-08

申请号：KR1019830004695

申请日：1983-10-04

Applicant: 한국과학기술연구원

Inventor： 김남득 ,  변시명 ,  김현표 ,  김성완

IPC: A61K38/43

公开(公告)号：KR1019890000662A

公开(公告)日：1989-03-15

申请号：KR1019870006560

申请日：1987-06-27

Applicant: 한국과학기술연구원

Inventor： 한문희 ,  정태화 ,  김지영 ,  강성만 ,  김성완 ,  하현정 ,  나도선

IPC: C12N15/00