公开(公告)号：KR100419592B1

公开(公告)日：2004-02-19

申请号：KR1020010074837

申请日：2001-11-29

Applicant: 한국과학기술연구원

Inventor： 한예선 ,  고훈영 ,  임혜원 ,  차주환 ,  김욱현 ,  백정호

IPC: C12N15/53

CPC classification number: C12N9/0036

Abstract: The invention is a gene coding for a quinone oxidoreductase from Kluyveromyces marxianus and a protein having an amino acid sequence expressed therefrom, which can be advantageously used in a reduction reaction of a quinone compound and synthesis of intermediates for a biologically active compound.

Abstract translation: 本发明是编码来自马克斯克鲁维酵母的醌氧化还原酶的基因和具有由其表达的氨基酸序列的蛋白质，其可以有利地用于醌化合物的还原反应和用于生物活性化合物的中间体的合成。

公开(公告)号：KR1020040011882A

公开(公告)日：2004-02-11

申请号：KR1020020045165

申请日：2002-07-31

Applicant: 한국과학기술연구원

Inventor： 한예선 ,  한수진 ,  백정호 ,  신평균 ,  정찬성 ,  성문희 ,  조현성 ,  이병석

IPC: C12N15/55

CPC classification number: C12N9/20 ,  C07K14/212 ,  C12N15/75 ,  C12Y301/01003

Abstract: PURPOSE: Provided are a gene coding lipase of Acinetobacter species SY-01 with stereoselective substrate specificity and a protein expressed therefrom. CONSTITUTION: A gene is characterized by coding amino acid sequence of lipase of Acinetobacter species SY-01, represented by the SEQ ID NO:2. A transformed Bacillus subtilis 168/pSYLipSM(1) (KCTC 10267BP) is obtained by transformation with a recombinant vector plasmid pSYLipSM(1) containing the gene coding the lipase of Acinetobacter species SY-01. The lipase of Acinetobacter species SY-01 is manufactured by the steps of culturing the transformed bacillus subtilis, and isolating and purifying expressed lipase of Acinetobacter species SY-01 from the cultured cell.

Abstract translation: 目的：提供具有立体选择底物特异性的不动杆菌属SY-01的脂肪酶基因和由其表达的蛋白质。 构成：基因的特征在于编码由SEQ ID NO：2表示的不动杆菌属物种SY-01的脂肪酶的氨基酸序列。 通过用含有编码不动杆菌属SY-01的脂肪酶的基因的重组载体质粒pSYLipSM（1）转化获得转化的枯草芽孢杆菌168 / pSYLipSM（1）（KCTC 10267BP）。 通过培养转化的枯草芽孢杆菌并从培养的细胞中分离和纯化不动杆菌属SY-01的表达脂肪酶的步骤制造不动杆菌属物种SY-01的脂肪酶。

公开(公告)号：KR1020030044179A

公开(公告)日：2003-06-09

申请号：KR1020010074837

申请日：2001-11-29

Applicant: 한국과학기술연구원

Inventor： 한예선 ,  고훈영 ,  임혜원 ,  차주환 ,  김욱현 ,  백정호

IPC: C12N15/53

CPC classification number: C12N9/0036

Abstract: PURPOSE: A quinone oxidoreductase gene from Kluyveromyces marxianus is provided to be used for the reduction reaction of the quinone compounds and for the intermediate synthesis of the biologically active compounds by way of the excellent quinone reduction activity thereof. CONSTITUTION: A gene codes the amino acid sequence of Kluyveromyces marxianus quinone oxidoreductase(kmQOR) presented at the sequence No. 2. The kmQOR has a molecular weight of about 42kDa. A recombinant vector contains the gene coded with the kmQOR, and is determined to be plasmid pQOR22b. An E. coli is transformed into the recombinant vector. In a method of preparing the kmQOR, the transformed E. coli is first cultured, and β-D-isopropyl-D-thiogalactopyranoside (IPTG) is then added to the cultured E. coli to induce the expression of kmQOR therefrom. The expressed kmQOR is collected, and purified.

Abstract translation: 目的：提供马克斯克鲁维酵母的醌氧化还原酶基因，用于醌类化合物的还原反应和生物活性化合物的中间合成，其优异的醌还原活性。 构成：A基因编码序列号2中提供的马克斯基黄酮醌氧化还原酶（kmQOR）的氨基酸序列。kmQOR的分子量约为42kDa。 重组载体含有用kmQOR编码的基因，并确定为质粒pQOR22b。 将大肠杆菌转化入重组载体。 在制备kmQOR的方法中，首先培养转化的大肠杆菌，然后将β-D-异丙基-D-硫代吡喃半乳糖苷（IPTG）加入到培养的大肠杆菌中以诱导其表达KmQOR。 收集并纯化表达的kmQOR。

公开(公告)号：KR100468547B1

公开(公告)日：2005-01-29

申请号：KR1020020045165

申请日：2002-07-31

Applicant: 한국과학기술연구원

Inventor： 한예선 ,  한수진 ,  백정호 ,  신평균 ,  정찬성 ,  성문희 ,  조현성 ,  이병석

IPC: C12N15/55

Abstract: PURPOSE: Provided are a gene coding lipase of Acinetobacter species SY-01 with stereoselective substrate specificity and a protein expressed therefrom. CONSTITUTION: A gene is characterized by coding amino acid sequence of lipase of Acinetobacter species SY-01, represented by the SEQ ID NO:2. A transformed Bacillus subtilis 168/pSYLipSM(1) (KCTC 10267BP) is obtained by transformation with a recombinant vector plasmid pSYLipSM(1) containing the gene coding the lipase of Acinetobacter species SY-01. The lipase of Acinetobacter species SY-01 is manufactured by the steps of culturing the transformed bacillus subtilis, and isolating and purifying expressed lipase of Acinetobacter species SY-01 from the cultured cell.

Abstract translation: 目的：提供具有立体选择性底物特异性的不动杆菌种SY-01的编码脂肪酶的基因和由其表达的蛋白质。 组成：基因的特征在于编码不动杆菌属物种SY-01的脂肪酶的氨基酸序列，由SEQ ID NO：2表示。 通过用含有编码不动杆菌属物种SY-01的脂肪酶的基因的重组载体质粒pSYLipSM（1）转化获得转化的枯草芽孢杆菌168 / pSYLipSM（1）（KCTC 10267BP）。 不动杆菌菌种SY-01的脂肪酶是通过培养转化的枯草芽孢杆菌，从培养细胞中分离纯化不动杆菌菌种SY-01的表达脂肪酶。