公开(公告)号：KR100347289B1

公开(公告)日：2002-08-07

申请号：KR1019990020954

申请日：1999-06-07

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  박호군 ,  정선주 ,  엄태연

IPC: C07H21/02

Abstract: 본발명은암세포의시알릴루이스 X (sLeX)에높은특이성과친화성으로접합하는 RNA서열및 이를이용한 sLeX의탐지방법을제공한다. 시알릴루이스 X (sLeX)는염증과암의전이에관련된세포표면표적자이고, 본발명의선택된 RNA는그 접합강도나분자의특이성면에서우월한결과를가지므로, 이는암세포를특이적으로인식할수 있어암세포의탐지화및 암치료제의개발에획기적인결과를줄 수있는분자가될 수있는잠재력을가지고있다.

公开(公告)号：KR1020010001607A

公开(公告)日：2001-01-05

申请号：KR1019990020954

申请日：1999-06-07

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  박호군 ,  정선주 ,  엄태연

IPC: C07H21/02

Abstract: PURPOSE: Provided is an RNA sequence, which binds to siallyl Lewis (sLe X), of a cancer cell with high specificity and affinity. Also, a method for detecting sLe X using the RNA sequence is provided. The RNA is excellent in binding strength or molecular specificity, therefore, it is useful in detecting the cancer cell and in developing a therapeutic agent thereof. CONSTITUTION: The RNA is selected from the group consisting of SEQ ID NO : 1 - SEQ ID NO : 19 and has the binding specificity and the affinity to sLe X which is a cell surface marker related to an inflammation and a metastasis. The RNA sequence is selected by the steps of preparing the RNA library, fixing the sLe X with CNBr-activating Sepharose 4B and carrying out a systematic evolution of ligand by exponential enrichment (SELEX) method. The detection method of the sLe X comprises the steps of : reacting the sLe X with the RNA sequence selected from SEQ ID NO : 1 - SEQ ID NO : 19; and assaying the RNA bound specifically the sLe X to detect the sLe X.

Abstract translation: 目的：提供具有高特异性和亲和力的癌细胞的与siall Lewis（sLe X）结合的RNA序列。 另外，提供了使用RNA序列检测sLe X的方法。 RNA结合强度或分子特异性优异，因此可用于检测癌细胞和开发其治疗剂。 构成：RNA选自SEQ ID NO：1 - SEQ ID NO：19，并且具有与作为与炎症和转移相关的细胞表面标志物的sLe X的结合特异性和亲和力。 通过制备RNA文库，用CNBr活化的Sepharose 4B固定sLe X并通过指数富集（SELEX）方法进行配体的系统进化来选择RNA序列。 sLe X的检测方法包括以下步骤：使sLe X与选自SEQ ID NO：1 - SEQ ID NO：19的RNA序列反应; 并测定特异性结合sLe X以检测sLe X的RNA。