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公开(公告)号：KR100346136B1
公开(公告)日：2002-08-01
申请号：KR1019990020951
申请日：1999-06-07
Applicant: 한국과학기술연구원
IPC: C07H21/02
Abstract: 본발명은친지성물질, 특히콜레스테롤또는이의유도체에특이적으로결합하는 RNA서열을제공하고, 또한친지성물질, 특히콜레스테롤또는이의유도체에특이적으로결합하고콜레스테롤에스터라제활성을갖는 RNA서열을제공하며, 이러한서열을이용하여미량의친지성물질, 특히 LDL을탐지하는방법및 이를제거하는방법을제공한다. 종래에밝혀진대부분의 RNA가친수성물질에결합함에비해친지성물질에결합가능한 RNA서열을밝힘으로써이를이용한미량의친지성물질을정량할수 있으며, 상기 RNA서열이콜레스테롤에스터라제활성을갖는성질을이용하여본발명의 RNA서열은환경호르몬에의한생체내의작용을억제할수 있는억제제로사용될수 있을것이며동맥경화와고혈압의근본적인원인인혈류내의 LDL 덩어리를제거하기위한수단으로사용될수 있고 RNA로이루어진인공촉매로서부작용이없는치료제로개발할수 있어그 이용분야가매우넓다.
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公开(公告)号：KR100336620B1
公开(公告)日：2002-05-16
申请号：KR1020000004479
申请日：2000-01-29
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: 본 발명에서는 안티-DNA 자가 항체(antibody specific to anti-DNA)에 대한 RNA서열을 제공하며, 선택된 RNA는 안티-DNA 자가항체에 결합하는 이중사슬 DNA 보다 10,000배 정도 강한 결합력을 나타낼뿐만 아니라 경쟁적 효소결합 면역 방출(competitive ELISA) 분석과 면역 침전 분석결과 안티-DNA 자가 항체에 특이적으로 결합하기 때문에 선택된 RNA가 자가항체를 충분히 중화시킬 수 있어 자기면역질환의 안티-DNA 자가항체를 억제할 수 있는 치료제로 사용할 수 있다.
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公开(公告)号：KR100336619B1
公开(公告)日：2002-05-16
申请号：KR1020000004317
申请日：2000-01-28
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: 본발명은 무작위 RNA라이브러리로부터 N-아세틸뉴라민-락토스에 큰 특이성 및 접합력으로 결합하는 RNA분자 및 이의 선택, 클로닝 및 염기서열분석 방법을 제공하며, 이 RNA 분자가 선택적으로 강하게 당을 인식하는 성질을 이용하여 특이적인 암의 치료, 특이적인 염증을 수반하는 모든 질병의 치료제로서 유용하며, 또한 선택적인 약수송 체계를 제조하는데 유용하다.
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公开(公告)号：KR1020010002016A
公开(公告)日：2001-01-05
申请号：KR1019990021582
申请日：1999-06-10
Applicant: 한국과학기술연구원
IPC: C12Q1/00
Abstract: PURPOSE: A method for quantifying sugar attached to a solid surface is provided to precisely quantifying the sugar through a gentle reaction by using an enzyme for separating the sugar from a reporter substance without any preprocessing procedure. CONSTITUTION: A method for quantifying sugar attached to a solid surface includes the steps of attaching sugar coupled with reporter molecules such as p-nitrophenyle on a surface of a solid such as a hydrogel selected from Cephalos or Agarose, dissolving glycoside coupling between the sugar and the reporter molecules with an enzyme such as glucosidase, amyloglucosidase, cellulase, fucosidase, mannosidase and galactosidase, and quantifying the dissolved reporter molecules
Abstract translation: 目的：提供一种定量连接到固体表面的糖的方法，通过使用酶从报告物质中分离糖而无需任何预处理程序，通过温和反应精确定量糖。 构成：用于定量连接到固体表面的糖的方法包括将糖与诸如对硝基苯的报告分子连接的糖连接到固体表面上的步骤，例如选自头孢菌素或琼脂糖的水凝胶，将糖与糖和糖 报告分子用酶如葡糖苷酶，淀粉葡糖苷酶，纤维素酶，岩藻糖苷酶，甘露糖苷酶和半乳糖苷酶分子，并定量溶解的报道分子
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Patent Agency Ranking
公开(公告)号：KR100428469B1
公开(公告)日：2004-04-27
申请号：KR1020010020421
申请日：2001-04-17
Applicant: 한국과학기술연구원
IPC: C07K7/08
CPC classification number: C07K7/08
Abstract: The present invention relates to carbohydrate-specific peptides, which have a strong affinity and preparation method thereof. Particularly, the present invention relates to carbohydrate-specific peptides, which have been developed to have an increased strong affinity to carbohydrates by the multiple combinations of peptides having carbohydrates-specific amino acid sequences and preparation method thereof. Carbohydrate-specific peptides of the present invention have a very strong affinity under micromol, so that they can shut off recognition between cells transferred by specific carbohydrates, which means they can be used for the treatment of diseases mediated by recognition between cells and used as a diagnostic kit which diagnoses diseases by confirming the existence of any specific carbohydrate.
Abstract translation: 本发明涉及具有强亲和力的碳水化合物特异性肽及其制备方法。 特别地，本发明涉及通过具有碳水化合物特异性氨基酸序列的肽的多种组合具有增强的对碳水化合物的强亲和力的碳水化合物特异性肽及其制备方法。 本发明的碳水化合物特异性肽在微摩尔下具有非常强的亲和力，从而它们可以关闭由特定碳水化合物转移的细胞之间的识别，这意味着它们可以用于治疗由细胞间识别介导的疾病并用作 通过确认任何特定碳水化合物的存在来诊断疾病的诊断试剂盒。
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公开(公告)号：KR100426612B1
公开(公告)日：2004-04-08
申请号：KR1019990021582
申请日：1999-06-10
Applicant: 한국과학기술연구원
IPC: C12Q1/00
Abstract: PURPOSE: A method for quantifying sugar attached to a solid surface is provided to precisely quantifying the sugar through a gentle reaction by using an enzyme for separating the sugar from a reporter substance without any preprocessing procedure. CONSTITUTION: A method for quantifying sugar attached to a solid surface includes the steps of attaching sugar coupled with reporter molecules such as p-nitrophenyle on a surface of a solid such as a hydrogel selected from Cephalos or Agarose, dissolving glycoside coupling between the sugar and the reporter molecules with an enzyme such as glucosidase, amyloglucosidase, cellulase, fucosidase, mannosidase and galactosidase, and quantifying the dissolved reporter molecules
Abstract translation: 目的：提供一种量化附着于固体表面的糖的方法，以通过使用用于从报道物质分离糖的酶进行温和反应来精确定量糖，而无需任何预处理程序。 构成：定量附着于固体表面的糖的方法包括以下步骤：将糖与报道分子（例如对硝基苯基）偶联在固体（例如选自头孢菌素或琼脂糖的水凝胶）的表面上，将糖与糖之间的糖苷偶联 报道分子与酶如葡糖苷酶，淀粉葡糖苷酶，纤维素酶，岩藻糖苷酶，甘露糖苷酶和半乳糖苷酶，并定量溶解的报道分子
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公开(公告)号：KR1020020080691A
公开(公告)日：2002-10-26
申请号：KR1020010020421
申请日：2001-04-17
Applicant: 한국과학기술연구원
IPC: C07K7/08
CPC classification number: C07K7/08
Abstract: PURPOSE: Provided are carbohydrate-specific peptides which have a strong affinity and a preparation method thereof. In particular, the carbohydrate-specific peptides are prepared by the multiple bond of peptides having a carbohydrate-specific sequence to increase an affinity. CONSTITUTION: The carbohydrate-specific peptides which have a strong affinity is characteristically prepared by the steps of; finding out peptides, which bind to carbohydrates, on the surface of a cell; and preparing peptides having a strong affinity to carbohydrates being on the surface of a cell by manufacturing a polymer of the peptides, wherein the polymer is manufactured by linking an amino acid having at least two amino residues to a peptide.
Abstract translation: 目的：提供具有强亲和力的碳水化合物特异性肽及其制备方法。 特别地，通过具有碳水化合物特异性序列的肽的多重键来制备碳水化合物特异性肽以增加亲和力。 构成：具有强亲和力的碳水化合物特异性肽通过以下步骤特征性地制备： 在细胞表面发现结合碳水化合物的肽; 并且通过制备肽的聚合物制备对碳水化合物具有强亲和力的肽，其中所述聚合物通过将具有至少两个氨基残基的氨基酸连接到肽来制备。
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公开(公告)号：KR1020010076989A
公开(公告)日：2001-08-17
申请号：KR1020000004479
申请日：2000-01-29
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: PURPOSE: A RNA molecule binding specifically to an antibody specific to anti-DNA is provided, which can be used as a therapeutic agent suppressing the antibody specific to anti-DNA in auto immune disease, since the selected RNA neutralizes the autoantibody sufficiently. CONSTITUTION: A RNA molecule binding specifically to an antibody specific to anti-DNA that causes auto immune disease is selected from the group consisting of sequence ID. No.1 to sequence ID.No.14. It shows bindability 10000 times as strong as double strand DNA and binds to an antibody specific to anti-DNA according to competitive ELISA analysis and immunoprecipitation analysis.
Abstract translation: 目的：提供特异性结合抗DNA特异性抗体的RNA分子，因为所选择的RNA能够充分中和自身抗体，可用作抑制自身免疫疾病中抗DNA特异性抗体的治疗剂。 构成：特异性结合引起自身免疫疾病的抗DNA特异性抗体的RNA分子选自序列ID。 序号为1号。 它显示出与双链DNA相当强的10000倍的结合能力，并根据竞争性ELISA分析和免疫沉淀分析结合抗DNA特异性抗体。
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公开(公告)号：KR1020010001607A
公开(公告)日：2001-01-05
申请号：KR1019990020954
申请日：1999-06-07
Applicant: 한국과학기술연구원
IPC: C07H21/02
Abstract: PURPOSE: Provided is an RNA sequence, which binds to siallyl Lewis (sLe X), of a cancer cell with high specificity and affinity. Also, a method for detecting sLe X using the RNA sequence is provided. The RNA is excellent in binding strength or molecular specificity, therefore, it is useful in detecting the cancer cell and in developing a therapeutic agent thereof. CONSTITUTION: The RNA is selected from the group consisting of SEQ ID NO : 1 - SEQ ID NO : 19 and has the binding specificity and the affinity to sLe X which is a cell surface marker related to an inflammation and a metastasis. The RNA sequence is selected by the steps of preparing the RNA library, fixing the sLe X with CNBr-activating Sepharose 4B and carrying out a systematic evolution of ligand by exponential enrichment (SELEX) method. The detection method of the sLe X comprises the steps of : reacting the sLe X with the RNA sequence selected from SEQ ID NO : 1 - SEQ ID NO : 19; and assaying the RNA bound specifically the sLe X to detect the sLe X.
Abstract translation: 目的：提供具有高特异性和亲和力的癌细胞的与siall Lewis（sLe X）结合的RNA序列。 另外，提供了使用RNA序列检测sLe X的方法。 RNA结合强度或分子特异性优异，因此可用于检测癌细胞和开发其治疗剂。 构成：RNA选自SEQ ID NO：1 - SEQ ID NO：19，并且具有与作为与炎症和转移相关的细胞表面标志物的sLe X的结合特异性和亲和力。 通过制备RNA文库，用CNBr活化的Sepharose 4B固定sLe X并通过指数富集（SELEX）方法进行配体的系统进化来选择RNA序列。 sLe X的检测方法包括以下步骤：使sLe X与选自SEQ ID NO：1 - SEQ ID NO：19的RNA序列反应; 并测定特异性结合sLe X以检测sLe X的RNA。
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