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1.发明公开
公开(公告)号：KR1019990025976A
公开(公告)日：1999-04-06
申请号：KR1019970047869
申请日：1997-09-20
Applicant: 한국과학기술연구원
Abstract: 본 발명은 세라시아 마르세센스(Serratia Marcescens)의 배양액에서 분리한 프로디지오신 (Prodigiosin)의 면역억제 물질로서의 신규한 용도에 관한 것으로 조직 및 장기이식 등의 면역억제를 필요로하는 치료분야에서의 면역억제약물로서의 이용 및 이들의 기초기전연구를 위한 면역억제 약물로서의 이용 나아가 프로디지오신의 면역억제효과를 응용한 면역학 기초연구를 위한 면역억제 시료로서의 이용에 관한 것이다.
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公开(公告)号：KR100252197B1
公开(公告)日：2000-04-15
申请号：KR1019970047869
申请日：1997-09-20
Applicant: 한국과학기술연구원
CPC classification number: C12R1/43 , C12P17/165
Abstract: PURPOSE: Provided is prodigiosin isolated from culture broth of Serratia marcescens. The prodigiosin inhibits specifically immunogenecity of T cell and is used in immunosuppresive drug. CONSTITUTION: A Prodigiosin isolated from culture broth of Serratia marcescens B-1231 (KCTC 0386BP) is represented by the formula (I). The Prodigiosin is prepared by the following steps of: (a) cultivating Serratia marcescens B-1231 (KCTC 0386BP) in a medium comprised of soluble starch of 1%, glucose of 0.25, ammonium sulfate of 0.1%, potassium phosphate of 0.1%, magnesium sulfate- septuple crystal solution of 0.05%, calcium chloride of 0.1 %, sodium chloride of 0.3% at pH value of 7 and temperature of 28 deg.C for 62 hours; (b) mixing them with adding ethyl acetate in a same amount, thereto for 30 minutes, to obtain organic solvent layer; (c) concentrating the mixture under reduced pressure, to obtain a red material; (d) isolating and purifying an active material therefrom; and (e) purifying it by silicagel thin layer chromatography.
Abstract translation: 目的：提供从粘质沙雷氏菌的培养液中分离的天蛾素。 胎牛血清抑制T细胞的特异性免疫原性，并用于免疫抑制药物。 构成：从粘质沙雷氏菌B-1231（KCTC 0386BP）的培养肉汤中分离出的地高辛分子由式（I）表示。 Prodigiosin通过以下步骤制备：（a）在由1％的可溶性淀粉，0.25的硫酸铵，0.1％的磷酸钾，0.1％的磷酸钾组成的培养基中培养粘质沙雷氏菌B-1231（KCTC 0386BP） 0.5％硫酸镁 - 六分体晶体溶液，氯化钙0.1％，氯化钠0.3％，pH值7，温度28℃62小时; （b）将其与相同量的乙酸乙酯混合30分钟，得到有机溶剂层; （c）在减压下浓缩混合物，得到红色材料; （d）从其中分离和纯化活性物质; 和（e）通过硅胶薄层色谱纯化。
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