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公开(公告)号：KR100308521B1
公开(公告)日：2001-11-05
申请号：KR1019990015654
申请日：1999-04-30
Applicant: 한국과학기술연구원
Abstract: 본발명은형질전환대장균(Escherichia coli) 및이를이용한 D형또는 L형젖산의순수생산방법에관한것으로서, 더욱상세하게는유전공학기술을이용하여대사경로를변형시키므로초산및 숙신산생성경로를유전적으로결손시켜 D형젖산만을선택적으로생산할수 있는신균주대장균 JP203, 또는 L형젖산만을선택적으로생산할수 있는신균주대장균 JP204을얻고, 이균주들을호기적조건에서 1차생장시킨후, 젖산생산단계에서는 2차적으로배양조건을혐기적조건으로전환시켜배양함으로써, 보다효율적으로그리고보다높은수율로 D형또는 L형젖산만을선택적으로대량생산할수 있는방법에관한것이다.
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Patent Agency Ranking
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公开(公告)号：KR100312070B1
公开(公告)日：2001-11-03
申请号：KR1019990008677
申请日：1999-03-15
Applicant: 한국과학기술연구원
Inventor： 정흥채
IPC: C12N9/00
CPC classification number: C40B40/02 , C12N15/1037
Abstract: 본 발명은 고속 선별법을 포함하는 개선된 형질을 갖는 효소 변이체의 선별방법에 관한 것으로서, 더욱 상세하게는 효소 유전자의 돌연변이 유발단계; 돌연변이 유발된 효소 유전자를 표면 발현 단백질의 유전자를 포함하는 발현벡터에 결합한 다음, 미생물 숙주에 도입하는 효소 유전자 변이체 라이브러리의 구축단계; 구축된 효소 유전자 변이체 라이브러리의 미생물 숙주 표면으로의 발현단계; 그리고 증가된 증식속도 또는 효소활성을 나타내는 미생물 숙주의 탐색 및 선별 단계를 포함하는 효소 변이체의 선별방법에 관한 것으로서, 신속하게 효소 변이체의 탐색 및 선별단계를 수행할 수 있어 전체 과정이 용이하게 되는 효과가 있다.
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公开(公告)号：KR1020000060418A
公开(公告)日：2000-10-16
申请号：KR1019990008677
申请日：1999-03-15
Applicant: 한국과학기술연구원
Inventor： 정흥채
IPC: C12N9/00
CPC classification number: C40B40/02 , C12N15/1037
Abstract: PURPOSE: A preparation method of enzyme variants having improved traits is provided which comprises a high speed selection method therefore it is able to perform a speedy search step and a selection step of enzyme variants. CONSTITUTION: A method for preparation of enzyme variants comprises the following steps: (a) mutagenesis of enzyme gene; (b) insertion of the mutated enzyme gene into an expression vector having a surface-expression protein gene and then construction of enzyme gene variants library to be introduced into a microbe host; (c) expression of the enzyme gene variants library as the surface of microbe host; and (d) search and selection of microbe hosts exhibiting improved multiplication velocity or enzymatic activity.
Abstract translation: 目的：提供具有改善性状的酶变体的制备方法，其包括高速选择方法，因此能够进行酶变体的快速搜索步骤和选择步骤。 构成：酶变体的制备方法包括以下步骤：（a）酶基因的诱变; （b）将突变的酶基因插入具有表达表达蛋白基因的表达载体中，然后构建待引入微生物宿主的酶基因变体文库; （c）酶基因变体文库的表达作为微生物宿主的表面; 和（d）搜索和选择显示改善的倍增速度或酶活性的微生物宿主。
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公开(公告)号：KR1020000067653A
公开(公告)日：2000-11-25
申请号：KR1019990015654
申请日：1999-04-30
Applicant: 한국과학기술연구원
Abstract: PURPOSE: Transformed E. coli is provided and a method for producing selectively D-type or L-type of lactic acid in high yield is also provided by modifying the metabolism pathway of a transformant. CONSTITUTION: A novel mutant strain, E. coli JP 203 (KCTC 0599BP) produces D-type of lactic acid selectively by deleting the generation pathway of acetic acid and succinic acid. A novel mutant strain, E.coli JP204 (KCTC 0600BP) containing plasmid pLS65, produces only L-type of lactic acid. E. coli JP 203 (KCTC 0599BP) and E.coli JP204 (KCTC 0600BP) are cultured individually under aerobic condition primarily then cultured under anaerobic or oxygen free condition. Thereby, D-type or L-type of lactic acid is selectively produced in a high yield.
Abstract translation: 目的：提供转化的大肠杆菌，并且通过改变转化体的代谢途径也提供了以高产率选择性地生产D型或L-型乳酸的方法。 构成：一种新型突变菌株，大肠杆菌JP 203（KCTC 0599BP）通过缺失乙酸和琥珀酸的产生途径选择性地产生D-型乳酸。 含有质粒pLS65的新型突变菌株大肠杆菌JP204（KCTC0600BP）仅产生L型乳酸。 大肠杆菌JP 203（KCTC 0599BP）和大肠杆菌JP204（KCTC 0600BP）在需氧条件下分别培养，然后在无氧或无氧条件下培养。 由此，以高收率选择性地制造D型或L-型乳酸。
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公开(公告)号：KR1019960005086B1
公开(公告)日：1996-04-20
申请号：KR1019920011016
申请日：1992-06-24
Applicant: 한국과학기술연구원
IPC: C12N1/20
Abstract: When pseudomonas syringae(KCTC 1832) is cultivated in the fermentor including industrial microorganisms, yeast extract, soy pepton, and corn-steep liquor, the following processes can increase the productivity of the microorganism. (a) the process of limiting dissolved oxygen to 10-50 % with oxygen membrane; (b) the process of providing medium to maintain above 1% of glucose concentration; (c) the process of lowering culture temp. to 20-24 deg.C with dissolved oxygen maintained below 1ppm.
Abstract translation: 当在发酵罐中培育假单胞菌（KCTC 1832）时，包括工业微生物，酵母提取物，大豆蛋白和玉米浆，以下方法可以提高微生物的生产力。 （a）用氧气膜将溶解氧限制在10-50％的过程; （b）提供培养基以维持1％葡萄糖浓度的过程; （c）降低培养温度的过程 至20-24℃，溶解氧保持在1ppm以下。
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