公开(公告)号：KR100101780B1

公开(公告)日：1996-07-08

申请号：KR1019920011016

申请日：1992-06-24

Applicant: 한국과학기술연구원

Inventor： 반재구 ,  한문희 ,  김정하 ,  정흥채

IPC: C12N1/20

公开(公告)号：KR1019960005086B1

公开(公告)日：1996-04-20

申请号：KR1019920011016

申请日：1992-06-24

Applicant: 한국과학기술연구원

Inventor： 반재구 ,  한문희 ,  김정하 ,  정흥채

IPC: C12N1/20

Abstract: When pseudomonas syringae(KCTC 1832) is cultivated in the fermentor including industrial microorganisms, yeast extract, soy pepton, and corn-steep liquor, the following processes can increase the productivity of the microorganism. (a) the process of limiting dissolved oxygen to 10-50 % with oxygen membrane; (b) the process of providing medium to maintain above 1% of glucose concentration; (c) the process of lowering culture temp. to 20-24 deg.C with dissolved oxygen maintained below 1ppm.

Abstract translation: 当在发酵罐中培育假单胞菌（KCTC 1832）时，包括工业微生物，酵母提取物，大豆蛋白和玉米浆，以下方法可以提高微生物的生产力。 （a）用氧气膜将溶解氧限制在10-50％的过程; （b）提供培养基以维持1％葡萄糖浓度的过程; （c）降低培养温度的过程 至20-24℃，溶解氧保持在1ppm以下。

公开(公告)号：KR100049138B1

公开(公告)日：1992-02-18

申请号：KR1019890017861

申请日：1989-12-04

Applicant: 한국과학기술연구원

Inventor： 한문희 ,  홍주봉 ,  이혜주 ,  함경수 ,  김정하

IPC: C12N15/31

公开(公告)号：KR1019940000569A

公开(公告)日：1994-01-18

申请号：KR1019920011016

申请日：1992-06-24

Applicant: 한국과학기술연구원

Inventor： 반재구 ,  한문희 ,  김정하 ,  정흥채

IPC: C12N1/20

公开(公告)号：KR1019910012237A

公开(公告)日：1991-08-07

申请号：KR1019890017861

申请日：1989-12-04

Applicant: 한국과학기술연구원

Inventor： 한문희 ,  홍주봉 ,  이혜주 ,  함경수 ,  김정하

IPC: C12N15/31

Abstract: 내용 없음.

公开(公告)号：KR1019910007611B1

公开(公告)日：1991-09-28

申请号：KR1019890017861

申请日：1989-12-04

Applicant: 한국과학기술연구원

Inventor： 한문희 ,  홍주봉 ,  이혜주 ,  함경수 ,  김정하

IPC: C12N15/31

Abstract: A gene for coding ice nucleating protein is prepd. by culturing Pseudomonas syringae AL, KCTC 1832, separating its chromosomal DNA, treating with restriction enzyme Bgl II/EcoRI, applicating in agarose gel electrophoresis, moving nytran membrane, hybridizing with 32P-oligonucleotide, 5'- CCGTAACCGGCGGTCAACGTGC3' to obtain the selected DNA fragment, inserting into the Bam HI/EcoRI site of vector pBR 322 to obtain the recombinant DNA, amplifying in E. coli, separating the plasmid DNA, treating with Bgl II/ EcoRI to obtain the ice nucleus activating gene clone pGINP-1, inserting to the E. coli KCTC 8475 (I). The (I) is useful for prevention of freezing and/or frost damage of frozen food prodn. and preservation.

Abstract translation: 用于编码冰核蛋白的基因是制备的。 通过培养丁香假单胞菌AL，KCTC 1832，分离其染色体DNA，用限制酶Bgl II / EcoRI处理，应用于琼脂糖凝胶电泳，移动nttran膜，与32P-寡核苷酸杂交，5'-CCGTAACCGGCGGTCAACGTGC3'获得所选择的DNA片段 插入载体pBR 322的Bam HI / EcoRI位点，得到重组DNA，扩增大肠杆菌，分离质粒DNA，用Bgl II / EcoRI处理，得到冰核激活基因克隆pGINP-1，插入 大肠杆菌KCTC 8475（I）。 （I）可用于预防冷冻食品的冻结和/或霜冻损伤。 并保存。