公开(公告)号：KR100070671B1

公开(公告)日：1994-02-03

申请号：KR1019910007224

申请日：1991-05-03

Applicant: 한국과학기술연구원

Inventor： 홍주봉 ,  조혜선 ,  이혜주 ,  한문희 ,  나도선

IPC: C12N15/82

公开(公告)号：KR1019930009562B1

公开(公告)日：1993-10-07

申请号：KR1019910007224

申请日：1991-05-03

Applicant: 한국과학기술연구원

Inventor： 홍주봉 ,  조혜선 ,  이혜주 ,  한문희 ,  나도선

IPC: C12N15/82

Abstract: The method for producing human interleukin-2 protein in a tobacco tissue comprises: (a) inserting human interleukin-2 gene into a vector pBKS-1 to obtain a recombinant plasmid DNA; (b) transforming it into Agrobacterium tumefaciens; (c) adding the transformants into tobacco leaves callus and culturing at 25 deg.C for 1-2 days in a dark place; (d) washing it with MS medium and culturing in a selective medium containing kanamycin and carbenicillin; (e) culturing it to obtain a tobacco tissue (KCTC 8499P), producing human interleukin-2 protein.

Abstract translation: 在烟草组织中产生人白介素-2蛋白的方法包括：（a）将人白细胞介素-2基因插入载体pBKS-1中以获得重组质粒DNA; （b）将其转化为根癌土壤杆菌; （c）将转化体加入烟草叶愈伤组织，并在黑暗的地方在25℃下培养1-2天; （d）用MS培养基洗涤并在含有卡那霉素和羧苄青霉素的选择培养基中培养; （e）培养以获得烟草组织（KCTC 8499P），产生人白细胞介素-2蛋白。

公开(公告)号：KR1019920021712A

公开(公告)日：1992-12-18

申请号：KR1019910007224

申请日：1991-05-03

Applicant: 한국과학기술연구원

Inventor： 홍주봉 ,  조혜선 ,  이혜주 ,  한문희 ,  나도선

IPC: C12N15/82

Abstract: 내용 없음