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公开(公告)号：KR100426612B1
公开(公告)日：2004-04-08
申请号：KR1019990021582
申请日：1999-06-10
Applicant: 한국과학기술연구원
IPC: C12Q1/00
Abstract: PURPOSE: A method for quantifying sugar attached to a solid surface is provided to precisely quantifying the sugar through a gentle reaction by using an enzyme for separating the sugar from a reporter substance without any preprocessing procedure. CONSTITUTION: A method for quantifying sugar attached to a solid surface includes the steps of attaching sugar coupled with reporter molecules such as p-nitrophenyle on a surface of a solid such as a hydrogel selected from Cephalos or Agarose, dissolving glycoside coupling between the sugar and the reporter molecules with an enzyme such as glucosidase, amyloglucosidase, cellulase, fucosidase, mannosidase and galactosidase, and quantifying the dissolved reporter molecules
Abstract translation: 目的：提供一种量化附着于固体表面的糖的方法，以通过使用用于从报道物质分离糖的酶进行温和反应来精确定量糖，而无需任何预处理程序。 构成：定量附着于固体表面的糖的方法包括以下步骤：将糖与报道分子（例如对硝基苯基）偶联在固体（例如选自头孢菌素或琼脂糖的水凝胶）的表面上，将糖与糖之间的糖苷偶联 报道分子与酶如葡糖苷酶，淀粉葡糖苷酶，纤维素酶，岩藻糖苷酶，甘露糖苷酶和半乳糖苷酶，并定量溶解的报道分子
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2.发明公开
公开(公告)号：KR1020010001605A
公开(公告)日：2001-01-05
申请号：KR1019990020951
申请日：1999-06-07
Applicant: 한국과학기술연구원
IPC: C07H21/02
Abstract: PURPOSE: Provided is an RNA sequence which has a binding specificity to cholesterol or derivatives thereof and which has an activity of a cholesterol esterase. Also, provided is a detection method of a lipophilic substance, especially LDL resulting in arteriosclerosis and a hypertension using the RNA sequence. The sequence is useful in an inhibitor blocking a bioreaction caused by endocrine disruptors and is used as an artificial catalyst without any side effect. CONSTITUTION: The RNA sequence is selected from the group consisting of SEQ ID NO : 1 - SEQ ID NO : 11 and has the activity of a cholesterol esterase. The RNA sequence is prepared by a 131 bp DNA sequence comprising 70 nucleotides of random sequence as shown in Fig. 1, wherein T7 RNA polymerase of 5'-primer promotes an in vitro transcription and the site of a restriction enzyme is useful in identifying that the inserts have been incorporated into the sequence. The detection method of the lipophilic substance comprises the steps of : reacting the lipophilic substance with the RNA sequence selected from SEQ ID NO : 1 - SEQ ID NO : 11; and assaying the RNA bonded specifically with the lipophilic substance to detect the lipophilic substance.
Abstract translation: 目的：提供对胆固醇或其衍生物具有结合特异性并具有胆固醇酯酶活性的RNA序列。 此外，提供亲脂性物质的检测方法，特别是导致动脉硬化的LDL和使用RNA序列的高血压。 该序列可用于阻断由内分泌干扰物引起的生物反应的抑制剂，并用作人造催化剂而没有任何副作用。 构成：RNA序列选自SEQ ID NO：1〜SEQ ID NO：11，具有胆固醇酯酶的活性。 RNA序列由包含70个随机序列核苷酸的131bp DNA序列制备，如图1所示。 1，其中5'-引物的T7RNA聚合酶促进体外转录，并且限制酶的位点可用于鉴定插入片段已经并入序列。 亲脂性物质的检测方法包括以下步骤：使亲脂性物质与选自SEQ ID NO：1〜SEQ ID NO：11的RNA序列反应; 并测定与亲脂性物质特异性结合的RNA以检测亲脂性物质。
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公开(公告)号：KR1020010076898A
公开(公告)日：2001-08-17
申请号：KR1020000004317
申请日：2000-01-28
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: PURPOSE: RNA molecule conjugating with N-acethylneuramine-lactose is provided, which can be effectively used for the treatment of specific cancers or diseases followed by specific inflammation. CONSTITUTION: RNA molecule conjugating with N-acethylneuramine-lactose and having the base sequence of SEQ ID NO: 1 is isolated by the steps of: binding N-acethylneuramine lactose on the solid phase supporter by the covalent bond; binding RNA molecule with the N-acethylneuramine lactose fixed on the solid phase supporter; eluting the bound RNA library by using affinity chromatography; and repeating the steps to isolate RNA molecule strongly conjugating with N-acethylneuramine-lactose.
Abstract translation: 目的：提供与N-乙酰神经胺 - 乳糖共轭的RNA分子，可以有效地用于治疗特定的癌症或疾病，然后特异性炎症。 构成：通过以下步骤分离与N-乙酰甲基甘氨酸 - 乳糖共轭并具有SEQ ID NO：1的碱基序列的RNA分子，其通过共价键结合固相载体上的N-乙酰基三聚氰胺乳糖; 结合RNA分子与固定在固相载体上的N-乙酰基三聚氰胺乳糖; 通过亲和层析洗脱结合的RNA文库; 并重复步骤以分离与N-乙酰神经胺 - 乳糖强烈缀合的RNA分子。
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公开(公告)号：KR1020010001606A
公开(公告)日：2001-01-05
申请号：KR1019990020953
申请日：1999-06-07
Applicant: 한국과학기술연구원
IPC: C07H21/02
Abstract: PURPOSE: Provided are an RNA sequence which has a binding specificity to kanamycin A and a selection method of the RNA sequence having a high specificity to an antibiotic such as kanamycin A. Because the RNA sequence is readily used in reverse drug discovery, it can cut costs and is useful in searching a novel target molecule to the kanamycin A. CONSTITUTION: The RNA is selected from the group consisting of SEQ ID NO : 1 - SEQ ID NO : 8 and has a binding specificity to kanamycin A. The RNA sequence is prepared by a 131 bp DNA sequence comprising 70 nucleotides of random sequence as shown in Fig. 1, wherein T7 RNA polymerase of 5'-primer promotes an in vitro transcription and the site of a restriction enzyme is useful in identifying that the inserts have been incorporated into the sequence. The selection method of the RNA sequence having a high specificity to the antibiotic comprises reacting the antibiotic fixed to solid phase with 100-200 bp of the RNA sequence ligated with 70 random nucleotides at 5' or 3' end thereof using a systematic evolution of ligand by exponential enrichment (SELEX) method.
Abstract translation: 目的：提供对卡那霉素A具有结合特异性的RNA序列和对抗生素如卡那霉素A具有高特异性的RNA序列的选择方法。因为RNA序列容易用于反向药物发现，因此可以切割 成本并且可用于在卡那霉素A中搜索新的靶分子。构成：RNA选自SEQ ID NO：1 - SEQ ID NO：8，并且对卡那霉素A具有结合特异性.RNA序列是 由包含70个核苷酸的随机序列的131bp DNA序列制备，如图1所示。 1，其中5'-引物的T7RNA聚合酶促进体外转录，并且限制酶的位点可用于鉴定插入片段已被并入序列。 对抗生素具有高特异性的RNA序列的选择方法包括将固定在固相上的抗生素与其5'或3'末端与70个随机核苷酸连接的DNA序列的100-200bp反应，使用配体的系统进化 通过指数富集（SELEX）方法。
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公开(公告)号：KR100346136B1
公开(公告)日：2002-08-01
申请号：KR1019990020951
申请日：1999-06-07
Applicant: 한국과학기술연구원
IPC: C07H21/02
Abstract: 본발명은친지성물질, 특히콜레스테롤또는이의유도체에특이적으로결합하는 RNA서열을제공하고, 또한친지성물질, 특히콜레스테롤또는이의유도체에특이적으로결합하고콜레스테롤에스터라제활성을갖는 RNA서열을제공하며, 이러한서열을이용하여미량의친지성물질, 특히 LDL을탐지하는방법및 이를제거하는방법을제공한다. 종래에밝혀진대부분의 RNA가친수성물질에결합함에비해친지성물질에결합가능한 RNA서열을밝힘으로써이를이용한미량의친지성물질을정량할수 있으며, 상기 RNA서열이콜레스테롤에스터라제활성을갖는성질을이용하여본발명의 RNA서열은환경호르몬에의한생체내의작용을억제할수 있는억제제로사용될수 있을것이며동맥경화와고혈압의근본적인원인인혈류내의 LDL 덩어리를제거하기위한수단으로사용될수 있고 RNA로이루어진인공촉매로서부작용이없는치료제로개발할수 있어그 이용분야가매우넓다.
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Patent Agency Ranking
公开(公告)号：KR100336619B1
公开(公告)日：2002-05-16
申请号：KR1020000004317
申请日：2000-01-28
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: 본발명은 무작위 RNA라이브러리로부터 N-아세틸뉴라민-락토스에 큰 특이성 및 접합력으로 결합하는 RNA분자 및 이의 선택, 클로닝 및 염기서열분석 방법을 제공하며, 이 RNA 분자가 선택적으로 강하게 당을 인식하는 성질을 이용하여 특이적인 암의 치료, 특이적인 염증을 수반하는 모든 질병의 치료제로서 유용하며, 또한 선택적인 약수송 체계를 제조하는데 유용하다.
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公开(公告)号：KR1020010002016A
公开(公告)日：2001-01-05
申请号：KR1019990021582
申请日：1999-06-10
Applicant: 한국과학기술연구원
IPC: C12Q1/00
Abstract: PURPOSE: A method for quantifying sugar attached to a solid surface is provided to precisely quantifying the sugar through a gentle reaction by using an enzyme for separating the sugar from a reporter substance without any preprocessing procedure. CONSTITUTION: A method for quantifying sugar attached to a solid surface includes the steps of attaching sugar coupled with reporter molecules such as p-nitrophenyle on a surface of a solid such as a hydrogel selected from Cephalos or Agarose, dissolving glycoside coupling between the sugar and the reporter molecules with an enzyme such as glucosidase, amyloglucosidase, cellulase, fucosidase, mannosidase and galactosidase, and quantifying the dissolved reporter molecules
Abstract translation: 目的：提供一种定量连接到固体表面的糖的方法，通过使用酶从报告物质中分离糖而无需任何预处理程序，通过温和反应精确定量糖。 构成：用于定量连接到固体表面的糖的方法包括将糖与诸如对硝基苯的报告分子连接的糖连接到固体表面上的步骤，例如选自头孢菌素或琼脂糖的水凝胶，将糖与糖和糖 报告分子用酶如葡糖苷酶，淀粉葡糖苷酶，纤维素酶，岩藻糖苷酶，甘露糖苷酶和半乳糖苷酶分子，并定量溶解的报道分子
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公开(公告)号：KR100436564B1
公开(公告)日：2004-06-19
申请号：KR1020010035802
申请日：2001-06-22
Applicant: 한국과학기술연구원
IPC: C07K7/06
Abstract: PURPOSE: cAMP-specific peptides using phage display peptide library are provided. The cAMP-specific peptide can be useful for treatment of diseases associated with cAMP-mediated signal transfer and for quantification of cAMP. CONSTITUTION: The cAMP-specific peptides are provided, wherein the cAMP-specific peptides are peptide motive molecules consisting of 7 amino acids, having the amino acid sequences selected from the group consisting of from SEQ ID NO: 1 to SEQ ID NO: 28 or from SEQ ID NO: 81 to SEQ ID NO: 116, or peptide motive molecules consisting of 10 amino acids, having the amino acid sequences selected from the group consisting of from SEQ ID NO: 29 to SEQ ID NO: 80. The cDNAs encoding cAMP-specific proteins are selected from human cDNA libraries and have the nucleotide sequences selected from the group consisting of from SEQ ID NO: 117 to SEQ ID NO: 120. The proteins are encoded by the cDNAs selected from human cDNA libraries and have the amino acid sequences selected from the group consisting of from SEQ ID NO: 121 to SEQ ID NO: 128.
Abstract translation: 目的：提供使用噬菌体展示肽文库的cAMP特异性肽。 cAMP特异性肽可用于治疗与cAMP介导的信号转移相关的疾病和定量cAMP。 构成：提供了cAMP特异性肽，其中cAMP特异性肽是由7个氨基酸组成的肽动机分子，具有选自SEQ ID NO：1至SEQ ID NO：28的氨基酸序列或 SEQ ID NO：81至SEQ ID NO：116的氨基酸序列，或由具有选自SEQ ID NO：29至SEQ ID NO：80的氨基酸序列的10个氨基酸组成的肽动机分子。编码 cAMP特异性蛋白质选自人cDNA文库并具有选自SEQ ID NO：117至SEQ ID NO：120的核苷酸序列。蛋白质由选自人cDNA文库的cDNA编码并具有氨基酸 选自SEQ ID NO：121至SEQ ID NO：128的酸性序列。
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公开(公告)号：KR1020030000139A
公开(公告)日：2003-01-06
申请号：KR1020010035802
申请日：2001-06-22
Applicant: 한국과학기술연구원
IPC: C07K7/06
Abstract: PURPOSE: cAMP-specific peptides using phage display peptide library are provided. The cAMP-specific peptide can be useful for treatment of diseases associated with cAMP-mediated signal transfer and for quantification of cAMP. CONSTITUTION: The cAMP-specific peptides are provided, wherein the cAMP-specific peptides are peptide motive molecules consisting of 7 amino acids, having the amino acid sequences selected from the group consisting of from SEQ ID NO: 1 to SEQ ID NO: 28 or from SEQ ID NO: 81 to SEQ ID NO: 116, or peptide motive molecules consisting of 10 amino acids, having the amino acid sequences selected from the group consisting of from SEQ ID NO: 29 to SEQ ID NO: 80. The cDNAs encoding cAMP-specific proteins are selected from human cDNA libraries and have the nucleotide sequences selected from the group consisting of from SEQ ID NO: 117 to SEQ ID NO: 120. The proteins are encoded by the cDNAs selected from human cDNA libraries and have the amino acid sequences selected from the group consisting of from SEQ ID NO: 121 to SEQ ID NO: 128.
Abstract translation: 目的：提供使用噬菌体展示肽文库的cAMP特异性肽。 cAMP特异性肽可用于治疗与cAMP介导的信号转移相关的疾病和用于cAMP的定量。 构成：提供cAMP特异性肽，其中cAMP特异性肽是由7个氨基酸组成的肽运动分子，其具有选自SEQ ID NO：1至SEQ ID NO：28的氨基酸序列或 或具有选自SEQ ID NO：29至SEQ ID NO：80的氨基酸序列的10个氨基酸组成的肽运动分子。编码 cAMP特异性蛋白质选自人cDNA文库，并具有选自SEQ ID NO：117至SEQ ID NO：120的核苷酸序列。蛋白质由选自人cDNA文库的cDNA编码，并具有氨基 选自SEQ ID NO：121至SEQ ID NO：128的酸序列。
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