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    2.
    发明公开
    염색체 특정 부위 제거용 재조합 벡터 및 이를 이용한미생물 내 염색체 특정 부위의 제거방법 有权
    用于去除染色体的特定区域的重组载体和使用该染色体的微生物中特异性染色体区域的清除方法

    公开(公告)号:KR1020090086870A

    公开(公告)日:2009-08-14

    申请号:KR1020080012377

    申请日:2008-02-11

    Abstract: A recombinant vector for removing a specific site of chromosome is provided to sequencially and simply remove the specific gene site in a microorganism at once. A recombinant vector for removing a specific site of chromosome comprises a promoter (Para) which is induced by arabinose; a gene which encodes a protein related to lamda-red recombination; and promoter which is induced by a rhamnose. A method for removing the specific site of the chromosome using the recombinant vector comprises: a step of preparing a linear DNA fragment having homologous site A and B which is related to lamda-red recombination, I-SceI restriction enzyme cutting site, and homologous site C which is related to homologous recombination for removing selection marker; a step of introducing linear DNA fragement in transformed microorganism and replacing at specific site of microorganism chromosome; and a step of expressing I-SceI restriction enzyme and removing selection marker.

    Abstract translation: 提供了一种用于去除染色体特异性位点的重组载体,以便一次性地顺序并简单地除去微生物中的特异性基因位点。 用于去除染色体特异位点的重组载体包含由阿拉伯糖诱导的启动子(Para); 编码与lamda-red重组相关的蛋白质的基因; 和由鼠李糖诱导的启动子。 使用重组载体去除染色体的特定位点的方法包括:制备与lamda-red重组,I-SceI限制酶切割位点和同源位点相关的具有同源位点A和B的线性DNA片段的步骤 C与同源重组相关,用于去除选择标记; 在转化的微生物中引入线性DNA分解并在微生物染色体的特定部位置换的步骤; 以及表达I-SceI限制酶并除去选择标记的步骤。

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