Abstract:
본 발명은 염색체 특정 부위 제거용 재조합 벡터 및 이를 이용한 미생물 내 염색체 특정 부위의 제거방법에 관한 것으로, 더욱 상세하게는, 아라비노즈에 의해 유도되는 프로모터; 람다(λ)-레드(red) 재조합에 관여하는 단백질을 코딩하는 유전자; 람노즈에 의해 유도되는 프로모터; 및 I- Sce I 제한효소를 코딩하는 유전자;를 포함하는, 염색체 특정 부위 제거용 재조합 벡터로, 기존 방법에 비해 보다 간편하고 빠르게, 선별마커를 남기지 않고, 미생물 내 특정 유전자를 연속적으로 제거할 수 있다. 유전자 제거, 람다-레드 재조합 (λ-red recombination), 람노즈, 아라비노즈, 유전체 재조합
Abstract:
A recombinant vector for removing a specific site of chromosome is provided to sequencially and simply remove the specific gene site in a microorganism at once. A recombinant vector for removing a specific site of chromosome comprises a promoter (Para) which is induced by arabinose; a gene which encodes a protein related to lamda-red recombination; and promoter which is induced by a rhamnose. A method for removing the specific site of the chromosome using the recombinant vector comprises: a step of preparing a linear DNA fragment having homologous site A and B which is related to lamda-red recombination, I-SceI restriction enzyme cutting site, and homologous site C which is related to homologous recombination for removing selection marker; a step of introducing linear DNA fragement in transformed microorganism and replacing at specific site of microorganism chromosome; and a step of expressing I-SceI restriction enzyme and removing selection marker.
Abstract:
The present invention relates to a novel antimicrobial peptide, an antimicrobial composition including the same, a composition for preventing and treating infectious diseases including the peptide, a composition for skin regeneration including the peptide, and a pharmaceutical composition for wound healing, including the peptide. [Reference numerals] (AA) FSF survival rate (%);(BB) Peptide 1;(CC) Peptide 2;(DD) Peptide 3;(EE) Peptide 4;(FF) Peptide concentration (μg / ml)
Abstract:
PURPOSE: An amphipathic peptide-lipase conjugate containing an amphipathic peptide and lipase is provided to remarkably enhance lipase activity without separate surfactant. CONSTITUTION: An amphipathic peptide-lipase conjugate contains an amphipathic peptide and lipase. The amphipathic peptide is buforin IIb of sequence number 1, B0 of sequence number 2, paracine I of sequence number 3, KC of sequence number 4, or NRC of sequence number 5. The lipase is TliA(thermostable lipase) derived from Pseudomonas fluorescens or lipase M37 derived from Photobacterium lipolyticum. The amphipathic peptide is linked with the lipase by a linker.