자일라나아제를 생산하는 신규한 페니바실러스 sp. HPL-001 균주, 이로부터 분리한 신규한 자일라나아제 효소 및 이의 생산 방법
    1.
    发明公开
    자일라나아제를 생산하는 신규한 페니바실러스 sp. HPL-001 균주, 이로부터 분리한 신규한 자일라나아제 효소 및 이의 생산 방법 有权
    PAENIBACILLUS SP。 用于生产XYLANASE的HPL-001菌株及其生产的XYLANASE及其生产方法

    公开(公告)号:KR1020100021175A

    公开(公告)日:2010-02-24

    申请号:KR1020080079939

    申请日:2008-08-14

    Abstract: PURPOSE: A novel Paenibacillus sp. which produces xylanase is provided to perform lysis of xylan and develop bio fuel, substitution ingredient, special functional material, and bio polymer. CONSTITUTION: A novel Paenibacillus sp. HPL-001 (KCTC11365BP) which produces xylanase is isolated from soil containing sawdust and residue of waste wood remaining after cultivating mcushroom. The xylanase from Paenibacillus sp. HPL-001 has an amino acid sequence of sequence number 3. The xylanase is maximally active at 45°C-50°C and pH 6.0-7.0. A gene encoding the xylanase has a base of sequence number 2. A method for preparing xylanase comprises: a step of culturing the Paenibacillus sp. HPL-001 or its transformant in a medium; a step of centrifuging to obtain crude enzyme liquid; and a step of purifying xylanase from the crude enzyme liquid.

    Abstract translation: 目的:一种新型Paenibacillus sp。 提供生产木聚糖酶以进行木聚糖的裂解并开发生物燃料,替代成分,特殊功能材料和生物聚合物。 构成:一种新颖的Paenibacillus sp。 生产木聚糖酶的HPL-001(KCTC11365BP)是从含有木屑的土壤中分离出来的,并且在培育蘑菇后残留的废木材残留物。 来自Paenibacillus sp。的木聚糖酶 HPL-001具有序列号3的氨基酸序列。木聚糖酶在45℃-50℃和pH 6.0-7.0下具有最大的活性。 编码木聚糖酶的基因具有序列号2的碱基。制备木聚糖酶的方法包括:培养Paenibacillus sp。 HPL-001或其转化体在培养基中; 离心分离得到粗酶液的步骤; 以及从粗酶液中纯化木聚糖酶的步骤。

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