公开(公告)号：KR100832742B1

公开(公告)日：2008-05-27

申请号：KR1020070020220

申请日：2007-02-28

Applicant: 한국화학연구원

Inventor： 이현규 ,  박명순 ,  박창식 ,  박현규 ,  이현일 ,  임성윤

IPC: C12Q1/68

CPC classification number: C07K9/003 ,  C12Q1/6837 ,  C12Q1/6876

Abstract: A PNA(peptide nucleic acid) probe, a method for preparing a microarray using the same and a method for controlling quality of the PNA microarray are provided to measure the fixation efficiency non-destructively simultaneously on fixation without further operation. A PNA probe is characterized in that a 3-azidocumarine derivative represented by a formula(1) or (2) as a fluorescent linker is attached to an N-terminal, wherein m is from 0 to 4; n is from 1 to 4; p is from 0 to 3; q is 0 or 1; r is from 1 to 5; and X is O or NH. A method for preparing a PNA microarray comprises a step of fixing the PNA probe onto a solid substrate, which is modified to have an acetylene end, by spotting the PNA probe using a spotting solution including a Cu catalyst. A method for controlling quality of the PNA microarray comprises the steps of: (a) preparing the microarray by spotting the PNA probe onto the modified solid substrate; and (b) measuring the fixation efficiency by detecting a fluorescent signal emitted from spots of the PNA probe.

Abstract translation: 提供PNA（肽核酸）探针，使用其制备微阵列的方法和控制PNA微阵列质量的方法，用于在不进一步操作的情况下无损地同时测量固定效率。 PNA探针的特征在于，将由式（1）或（2）表示的3-叠氮胞菌素衍生物作为荧光接头连接到N-末端，其中m为0至4; n为1至4; p为0〜3; q为0或1; r为1〜5; X为O或NH。 制备PNA微阵列的方法包括通过使用包含Cu催化剂的斑点溶液点样PNA探针，将PNA探针固定在固体基底上的步骤，其被修饰为具有乙炔末端。 用于控制PNA微阵列质量的方法包括以下步骤：（a）通过将PNA探针点样到改性固体基质上来制备微阵列; 和（b）通过检测从PNA探针的斑点发射的荧光信号来测量固色效率。