公开(公告)号：WO9619587A2

公开(公告)日：1996-06-27

申请号：PCT/US9516627

申请日：1995-12-20

Applicant: ABBOTT LAB

Inventor： JOU TSUNG-HUI K ,  HOIJER JOANELL V

IPC: C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/53 ,  G01N37/00 ,  C07H21/00

CPC classification number: C12N15/1006 ,  C12Q1/6834

Abstract: The present invention provides a method for directly immobilizing an oligonucleotide to a support material. The method comprises the steps of contacting a solution of oligonucleotides with a solid support material and drying the oligonucleotide solution to the support material. Oligonucleotides and the solid support materials to which they are immobilized can be employed as capture reagents for immobilizing nucleic acid sequences which are complementary to the immobilized oligonucleotides. Hence, the hybridization capacity of directly immobilized oligonucleotides is maintained. Also provided are methods for determining the presence or amount of nucleic acid sequences in a test sample.

Abstract translation: 本发明提供了将寡核苷酸直接固定在载体材料上的方法。 该方法包括将寡核苷酸溶液与固体支持物质接触并将寡核苷酸溶液干燥至载体材料的步骤。 寡核苷酸及其固定的固体支持材料可用作固定与固定化寡核苷酸互补的核酸序列的捕获试剂。 因此，维持直接固定的寡核苷酸的杂交能力。 还提供了用于确定测试样品中核酸序列的存在或量的方法。

公开(公告)号：DE69121021T2

公开(公告)日：1997-02-27

申请号：DE69121021

申请日：1991-04-24

Applicant: ABBOTT LAB

Inventor： CHING SHANFUN ,  JOU TSUNG-HUI K ,  GORDON JULIAN

IPC: G01N33/543 ,  G01N33/558

Abstract: The novel analytical devices and methods of the present invention involve a solid phase material, such as a test strip, through which a test sample and a conjugate travel and sequentially contact at least two defined sites for the comparative display of immobilized conjugate. The two defined sites of the present invention include a capture site to which is attached a capture reagent and a conjugate recovery site to which is attached a conjugate recovery reagent. The immobilization of conjugate in these sites is relative to the amount of analyte in the test sample. As the amount of analyte in the sample increases, there is a decrease in the detectable conjugate at the capture site and a corresponding increase in detectable conjugate at the conjugate recovery site. The devices and methods can be used for detecting the presence or amount of an analyte in a test sample containing or suspected of containing the analyte of interest. Optionally, a polyvalent conjugate can be used.

公开(公告)号：AT140794T

公开(公告)日：1996-08-15

申请号：AT91106593

申请日：1991-04-24

Applicant: ABBOTT LAB

Inventor： CHING SHANFUN ,  JOU TSUNG-HUI K ,  GORDON JULIAN

IPC: G01N33/543 ,  G01N33/558 ,  G01N33/564 ,  G01N33/94

Abstract: The novel analytical devices and methods of the present invention involve a solid phase material, such as a test strip, through which a test sample and a conjugate travel and sequentially contact at least two defined sites for the comparative display of immobilized conjugate. The two defined sites of the present invention include a capture site to which is attached a capture reagent and a conjugate recovery site to which is attached a conjugate recovery reagent. The immobilization of conjugate in these sites is relative to the amount of analyte in the test sample. As the amount of analyte in the sample increases, there is a decrease in the detectable conjugate at the capture site and a corresponding increase in detectable conjugate at the conjugate recovery site. The devices and methods can be used for detecting the presence or amount of an analyte in a test sample containing or suspected of containing the analyte of interest. Optionally, a polyvalent conjugate can be used.

公开(公告)号：ES2091782T3

公开(公告)日：1996-11-16

申请号：ES90118019

申请日：1990-09-19

Applicant: ABBOTT LAB

Inventor： CHING SHAN FUN ,  GORDON JULIAN ,  JOU TSUNG-HUI K ,  STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY ,  ZAUN PETER

IPC: G01N30/92 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/558

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired due to the use of water based adhesives in making the laminates. Water based adhesives include AS73 from Adhesive Research, Inc, polyvinylacetate (PVA) and polyvinylpyrrolidone (PVP) among others.

公开(公告)号：DE69121021D1

公开(公告)日：1996-08-29

申请号：DE69121021

申请日：1991-04-24

Applicant: ABBOTT LAB

Inventor： CHING SHANFUN ,  JOU TSUNG-HUI K ,  GORDON JULIAN

IPC: G01N33/543 ,  G01N33/558

Abstract: The novel analytical devices and methods of the present invention involve a solid phase material, such as a test strip, through which a test sample and a conjugate travel and sequentially contact at least two defined sites for the comparative display of immobilized conjugate. The two defined sites of the present invention include a capture site to which is attached a capture reagent and a conjugate recovery site to which is attached a conjugate recovery reagent. The immobilization of conjugate in these sites is relative to the amount of analyte in the test sample. As the amount of analyte in the sample increases, there is a decrease in the detectable conjugate at the capture site and a corresponding increase in detectable conjugate at the conjugate recovery site. The devices and methods can be used for detecting the presence or amount of an analyte in a test sample containing or suspected of containing the analyte of interest. Optionally, a polyvalent conjugate can be used.

公开(公告)号：DE69027826D1

公开(公告)日：1996-08-22

申请号：DE69027826

申请日：1990-09-19

Applicant: ABBOTT LAB

Inventor： CHING SHAN FUN ,  GORDON JULIAN ,  JOU TSUNG-HUI K ,  STIMPSON DONALD ,  ZAKULA DOROTHY ,  ZAUN PETER

IPC: G01N30/92 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/558

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired due to the use of water based adhesives in making the laminates. Water based adhesives include AS73 from Adhesive Research, Inc, polyvinylacetate (PVA) and polyvinylpyrrolidone (PVP) among others.

公开(公告)号：CA2207629A1

公开(公告)日：1996-06-27

申请号：CA2207629

申请日：1995-12-20

Applicant: ABBOTT LAB

Inventor： JOU TSUNG-HUI K ,  HOIJER JOANELL V

IPC: C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/53 ,  G01N37/00

Abstract: The present invention provides a method for directly immobilizing an oligonucleotide to a support material. The method comprises the steps of contacting a solution of oligonucleotides with a solid support material and drying the oligonucleotide solution to the support material. Oligonucleotides and the solid support materials to which they are immobilized can be employed as capture reagents for immobilizing nucleic acid sequences which are complementary to the immobilized oligonucleotides. Hence, the hybridization capacity of directly immobilized oligonucleotides is maintained. Also provided are methods for determining the presence or amount of nucleic acid sequences in a test sample.

公开(公告)号：DE69027826T2

公开(公告)日：1997-02-13

申请号：DE69027826

申请日：1990-09-19

Applicant: ABBOTT LAB

Inventor： CHING SHAN FUN ,  GORDON JULIAN ,  JOU TSUNG-HUI K ,  STIMPSON DONALD ,  ZAKULA DOROTHY ,  ZAUN PETER

IPC: G01N30/92 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/558

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired due to the use of water based adhesives in making the laminates. Water based adhesives include AS73 from Adhesive Research, Inc, polyvinylacetate (PVA) and polyvinylpyrrolidone (PVP) among others.

公开(公告)号：ES2092523T3

公开(公告)日：1996-12-01

申请号：ES91106593

申请日：1991-04-24

Applicant: ABBOTT LAB

Inventor： CHING SHANFUN ,  JOU TSUNG-HUI K ,  GORDON JULIAN

IPC: G01N33/543 ,  G01N33/558

Abstract: The novel analytical devices and methods of the present invention involve a solid phase material, such as a test strip, through which a test sample and a conjugate travel and sequentially contact at least two defined sites for the comparative display of immobilized conjugate. The two defined sites of the present invention include a capture site to which is attached a capture reagent and a conjugate recovery site to which is attached a conjugate recovery reagent. The immobilization of conjugate in these sites is relative to the amount of analyte in the test sample. As the amount of analyte in the sample increases, there is a decrease in the detectable conjugate at the capture site and a corresponding increase in detectable conjugate at the conjugate recovery site. The devices and methods can be used for detecting the presence or amount of an analyte in a test sample containing or suspected of containing the analyte of interest. Optionally, a polyvalent conjugate can be used.

公开(公告)号：GR3020887T3

公开(公告)日：1996-11-30

申请号：GR960402250

申请日：1996-08-28

Applicant: ABBOTT LAB

Inventor： CHING SHAN FUN ,  GORDON JULIAN ,  JOU TSUNG-HUI K ,  STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY ,  ZAUN PETER

IPC: G01N30/92 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/558

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired due to the use of water based adhesives in making the laminates. Water based adhesives include AS73 from Adhesive Research, Inc, polyvinylacetate (PVA) and polyvinylpyrrolidone (PVP) among others.