公开(公告)号：AU629982B2

公开(公告)日：1992-10-15

申请号：AU6323790

申请日：1990-09-26

Applicant: ABBOTT LAB

Inventor： STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY

IPC: G01N33/543 ,  G01N30/92 ,  G01N33/545 ,  G01N33/548 ,  G01N30/90 ,  G01N33/52 ,  B01D69/10 ,  B01D71/34 ,  B01D63/08 ,  B32B23/08 ,  B32B23/04 ,  B32B27/16 ,  B32B27/06

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired, even when organic solvent based adhesives are used, due to the incorporation of anionic surfactants in a concentration range of from about 0.1% to about 3.5% (w/w) for nitrocellulose, and from about 2.0% to about 5.0% (w/w) for PVDP membranes.

公开(公告)号：AU630237B2

公开(公告)日：1992-10-22

申请号：AU6323590

申请日：1990-09-26

Applicant: ABBOTT LAB

Inventor： STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY

IPC: G01N33/543 ,  G01N30/92 ,  G01N33/545 ,  G01N33/548 ,  G01N30/90 ,  G01N33/52 ,  B01D69/10 ,  B01D71/10 ,  B01D63/08 ,  B32B23/04 ,  B32B23/08 ,  B32B27/06 ,  B32B27/16

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired, even when organic solvent based adhesives are used, due to the incorporation of an agent of the formula: R1N(CH3)+_3 . SO-_4R2 in a concentration range of from about 0.1% to about 0.2% (w/w) for nitrocellulose, and from about 2% to about 10% (w/w) for PVDF membranes. R1 and R2 are nonpolar, preferably alkyl moieties.

公开(公告)号：ES2091782T3

公开(公告)日：1996-11-16

申请号：ES90118019

申请日：1990-09-19

Applicant: ABBOTT LAB

Inventor： CHING SHAN FUN ,  GORDON JULIAN ,  JOU TSUNG-HUI K ,  STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY ,  ZAUN PETER

IPC: G01N30/92 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/558

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired due to the use of water based adhesives in making the laminates. Water based adhesives include AS73 from Adhesive Research, Inc, polyvinylacetate (PVA) and polyvinylpyrrolidone (PVP) among others.

公开(公告)号：CA2197321C

公开(公告)日：2009-01-27

申请号：CA2197321

申请日：1995-09-20

Applicant: ABBOTT LAB

Inventor： STIMPSON DONALD IRVINE ,  GORDON JULIAN ,  HOIJER JOANELL V

IPC: G01N21/77 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/55 ,  G01N33/543 ,  G01N33/553

Abstract: A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.

公开(公告)号：AU6323790A

公开(公告)日：1991-04-11

申请号：AU6323790

申请日：1990-09-26

Applicant: ABBOTT LAB

Inventor： STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY

IPC: G01N33/543 ,  G01N30/92 ,  G01N33/545 ,  G01N33/548 ,  G01N30/90 ,  G01N33/52 ,  B01D69/10 ,  B01D71/34 ,  B01D63/08 ,  B32B23/08 ,  B32B23/04 ,  B32B27/16 ,  B32B27/06

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired, even when organic solvent based adhesives are used, due to the incorporation of anionic surfactants in a concentration range of from about 0.1% to about 3.5% (w/w) for nitrocellulose, and from about 2.0% to about 5.0% (w/w) for PVDP membranes.

公开(公告)号：GR3020887T3

公开(公告)日：1996-11-30

申请号：GR960402250

申请日：1996-08-28

Applicant: ABBOTT LAB

Inventor： CHING SHAN FUN ,  GORDON JULIAN ,  JOU TSUNG-HUI K ,  STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY ,  ZAUN PETER

IPC: G01N30/92 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/558

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired due to the use of water based adhesives in making the laminates. Water based adhesives include AS73 from Adhesive Research, Inc, polyvinylacetate (PVA) and polyvinylpyrrolidone (PVP) among others.

公开(公告)号：DK0420021T3

公开(公告)日：1996-11-25

申请号：DK90118019

申请日：1990-09-19

Applicant: ABBOTT LAB

Inventor： CHING SHAN FUN ,  GORDON JULIAN ,  JOU TSUNG-HUI K ,  STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY ,  ZAUN PETER

IPC: G01N30/92 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/558

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired due to the use of water based adhesives in making the laminates. Water based adhesives include AS73 from Adhesive Research, Inc, polyvinylacetate (PVA) and polyvinylpyrrolidone (PVP) among others.

公开(公告)号：AT140537T

公开(公告)日：1996-08-15

申请号：AT90118019

申请日：1990-09-19

Applicant: ABBOTT LAB

Inventor： CHING SHAN FUN ,  GORDON JULIAN ,  JOU TSUNG-HUI K ,  STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY ,  ZAUN PETER

IPC: G01N30/92 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/558

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired due to the use of water based adhesives in making the laminates. Water based adhesives include AS73 from Adhesive Research, Inc, polyvinylacetate (PVA) and polyvinylpyrrolidone (PVP) among others.

公开(公告)号：AU630236B2

公开(公告)日：1992-10-22

申请号：AU6323490

申请日：1990-09-26

Applicant: ABBOTT LAB

Inventor： CHING SHAN FUN ,  GORDON JULIAN ,  JOU TSUNG-HUI K ,  STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY ,  ZAUN PETER

IPC: G01N30/92 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/558 ,  G01N30/90 ,  G01N33/52 ,  B01D63/08 ,  B01D71/10 ,  B01D69/10 ,  B32B23/04 ,  B32B23/08 ,  B32B27/06 ,  B32B27/16

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired due to the use of water based adhesives in making the laminates. Water based adhesives include AS73 from Adhesive Research, Inc, polyvinylacetate (PVA) and polyvinylpyrrolidone (PVP) among others.

公开(公告)号：AU6323590A

公开(公告)日：1991-04-11

申请号：AU6323590

申请日：1990-09-26

Applicant: ABBOTT LAB

Inventor： STIMPSON DONALD IRVINE ,  ZAKULA DOROTHY

IPC: G01N33/543 ,  G01N30/92 ,  G01N33/545 ,  G01N33/548 ,  G01N30/90 ,  G01N33/52 ,  B01D69/10 ,  B01D71/10 ,  B01D63/08 ,  B32B23/04 ,  B32B23/08 ,  B32B27/06 ,  B32B27/16

Abstract: Laminated porous membranes useful in chromatographic assay devices include nitrocellulose or polyvinylidene difluoride (PVDF) membranes laminated to supports. Despite lamination, the membranes remain wettable and the activity of biologically reactive reagents is not impaired, even when organic solvent based adhesives are used, due to the incorporation of an agent of the formula: R1N(CH3)+_3 . SO-_4R2 in a concentration range of from about 0.1% to about 0.2% (w/w) for nitrocellulose, and from about 2% to about 10% (w/w) for PVDF membranes. R1 and R2 are nonpolar, preferably alkyl moieties.