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    METHODS OF IMMOBILIZING OLIGONUCLEOTIDES TO SOLID SUPPORT MATERIALS AND METHODS OF USING SUPPORT BOUND OLIGONUCLEOTIDES
    1.
    发明申请
    METHODS OF IMMOBILIZING OLIGONUCLEOTIDES TO SOLID SUPPORT MATERIALS AND METHODS OF USING SUPPORT BOUND OLIGONUCLEOTIDES 审中-公开
    将寡核苷酸固定化为固体支持材料的方法和使用支持结合寡核苷酸的方法

    公开(公告)号：WO9619587A2

    公开(公告)日：1996-06-27

    申请号：PCT/US9516627

    申请日：1995-12-20

    Applicant: ABBOTT LAB

    Inventor： JOU TSUNG-HUI K ,  HOIJER JOANELL V

    IPC: C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/53 ,  G01N37/00 ,  C07H21/00

    CPC classification number: C12N15/1006 ,  C12Q1/6834

    Abstract: The present invention provides a method for directly immobilizing an oligonucleotide to a support material. The method comprises the steps of contacting a solution of oligonucleotides with a solid support material and drying the oligonucleotide solution to the support material. Oligonucleotides and the solid support materials to which they are immobilized can be employed as capture reagents for immobilizing nucleic acid sequences which are complementary to the immobilized oligonucleotides. Hence, the hybridization capacity of directly immobilized oligonucleotides is maintained. Also provided are methods for determining the presence or amount of nucleic acid sequences in a test sample.

    Abstract translation: 本发明提供了将寡核苷酸直接固定在载体材料上的方法。 该方法包括将寡核苷酸溶液与固体支持物质接触并将寡核苷酸溶液干燥至载体材料的步骤。 寡核苷酸及其固定的固体支持材料可用作固定与固定化寡核苷酸互补的核酸序列的捕获试剂。 因此，维持直接固定的寡核苷酸的杂交能力。 还提供了用于确定测试样品中核酸序列的存在或量的方法。

    OPTICAL WAVEGUIDE METHOD FOR DETECTING SPECIFIC BINDING EVENTS BY LIGHT SCATTERING
    2.
    发明专利
    OPTICAL WAVEGUIDE METHOD FOR DETECTING SPECIFIC BINDING EVENTS BY LIGHT SCATTERING 未知

    公开(公告)号：CA2197321C

    公开(公告)日：2009-01-27

    申请号：CA2197321

    申请日：1995-09-20

    Applicant: ABBOTT LAB

    Inventor： STIMPSON DONALD IRVINE ,  GORDON JULIAN ,  HOIJER JOANELL V

    IPC: G01N21/77 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/55 ,  G01N33/543 ,  G01N33/553

    Abstract: A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.

    OPTICAL WAVEGUIDE METHOD FOR DETECTING SPECIFIC BINDING EVENTS BY LIGHT SCATTERING
    3.
    发明专利
    OPTICAL WAVEGUIDE METHOD FOR DETECTING SPECIFIC BINDING EVENTS BY LIGHT SCATTERING 未知

    公开(公告)号：CA2197321A1

    公开(公告)日：1996-03-28

    申请号：CA2197321

    申请日：1995-09-20

    Applicant: ABBOTT LAB

    Inventor： STIMPSON DONALD I ,  GORDON JULIAN ,  HOIJER JOANELL V

    IPC: C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/55 ,  G01N33/543 ,  G01N33/553 ,  G01N21/77

    Abstract: A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.

    5.
    发明专利
    未知

    公开(公告)号：AT263967T

    公开(公告)日：2004-04-15

    申请号：AT95933864

    申请日：1995-09-20

    Applicant: ABBOTT LAB

    Inventor： STIMPSON DONALD I ,  GORDON JULIAN ,  HOIJER JOANELL V

    IPC: C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/55 ,  G01N33/543 ,  G01N33/553 ,  G01N21/77

    Abstract: A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.

    9.
    发明专利
    未知

    公开(公告)号：AT498123T

    公开(公告)日：2011-02-15

    申请号：AT04002064

    申请日：1995-09-20

    Applicant: ABBOTT LAB

    Inventor： STIMPSON DONALD I ,  GORDON JULIAN ,  HOIJER JOANELL V

    IPC: G01N21/77 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/55 ,  G01N33/543 ,  G01N33/553

    Abstract: A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.

    PROCEDIMIENTO QUE UTILIZA UN GUIA DE ONDAS OPTICO PARA DETECTAR ACONTECIMIENTOS DE UNION ESPECIFICA POR DIFUSION DE LA LUZ.
    10.
    发明专利
    PROCEDIMIENTO QUE UTILIZA UN GUIA DE ONDAS OPTICO PARA DETECTAR ACONTECIMIENTOS DE UNION ESPECIFICA POR DIFUSION DE LA LUZ. 未知

    公开(公告)号：ES2221930T3

    公开(公告)日：2005-01-16

    申请号：ES95933864

    申请日：1995-09-20

    Applicant: ABBOTT LAB

    Inventor： STIMPSON DONALD I ,  GORDON JULIAN ,  HOIJER JOANELL V

    IPC: C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/55 ,  G01N33/543 ,  G01N33/553 ,  G01N21/77

    Abstract: UN METODO DE ENSAYO DE LA UNION DE UNA GUIA DE ONDA LLEVA CONSIGO LA DETECCION DE LA DIFUSION DE LA LUZ DIRIGIDA EN LA GUIA DE ONDA. LA DIFUSION ES EL RESULTADO DE ETIQUETAS DE DIFUSION ESPECIFICAMENTE UNIDAS CON LA GUIA DE ONDA DENTRO DE LA PROFUNDIDAD DE PENETRACION DE UNA ONDA EVANESCENTE .LA GUIA DE ONDA PUEDE SER DE PLASTICO O VIDRIO TRANSPARENTE Y LA UNION SE HACE COMUNMENTE POR HIBRIDACION DE OLIGONUCLEOTIDOS O CAPTURA INMUNOLOGICA. LAS ETIQUETAS DE DIFUSION DE LA LUZ INCLUYEN METALES O NO METALES COLOIDALES, ENTRE LOS QUE SE INCLUYEN ORO, SELENIO Y LATEX. UN ELEMENTO DE ABSORCION DE LA LUZ CONSISTENTE EN UN TINTE O UNAS PARTICULAS CONCENTRADAS SE PUEDE UTILIZAR TAMBIEN PARA AUMENTAR LA SEÑAL. LA UNION A TIEMPO REAL Y LA DISOCIACION PUEDEN SER CONTROLADAS VISUALMENTE O POR UN DISPOSITIVO DE FORMACION DE IMAGENES DE VIDEO, COMO POR EJEMPLO, UNA CAMARA CCD Y UN PROGRAMA INFORMATICO DE AGARRE DEL ARMAZON. LOS DESEMPAREJAMIENTOS EN LA HIBRIDACION DE TAN SOLO UNA BASE PUEDEN SER DISTINGUIDOS POR CURVAS DE FUSION A TIEMPO REAL.

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