公开(公告)号：DE3003959A1

公开(公告)日：1980-08-14

申请号：DE3003959

申请日：1980-02-04

Applicant: ABBOTT LAB

Inventor： VOSS HOUSTON FREDERICK ,  PLATTNER JACOB ,  HERRIN THOMAS RAYMOND

IPC: C07F9/40 ,  C12Q1/46 ,  G01N33/532 ,  G01N33/542 ,  C07F9/201 ,  G01N33/16 ,  C07F9/65

Abstract: The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction. The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.

公开(公告)号：AU5476380A

公开(公告)日：1980-08-14

申请号：AU5476380

申请日：1980-01-21

Applicant: ABBOTT LAB

Inventor： VOSS HOUSTON FREDERICK ,  HERRIN THOMAS RAYMOND ,  PLATTNER JACOB

IPC: C07F9/40 ,  C12Q1/46 ,  G01N33/532 ,  G01N33/542 ,  C07G17/00 ,  C12Q1/00 ,  C07J41/00 ,  C07H15/22 ,  C07F9/65 ,  C07J43/00 ,  C07H23/00

Abstract: The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction. The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.

公开(公告)号：IT1130254B

公开(公告)日：1986-06-11

申请号：IT1967580

申请日：1980-02-04

Applicant: ABBOTT LAB

Inventor： VOSS HOUSTON FREDERICK ,  PLATTNER JACOB ,  HERRIN THOMAS RAYMOND

IPC: C07F9/40 ,  C12Q1/46 ,  G01N33/532 ,  G01N33/542 ,  G01N

Abstract: The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction. The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.

公开(公告)号：CH641569A5

公开(公告)日：1984-02-29

申请号：CH87780

申请日：1980-02-04

Applicant: ABBOTT LAB

Inventor： VOSS HOUSTON FREDERICK ,  PLATTNER JACOB ,  HERRIN THOMAS RAYMOND

IPC: C07F9/40 ,  C12Q1/46 ,  G01N33/532 ,  G01N33/542 ,  G01N33/54

公开(公告)号：AU528592B2

公开(公告)日：1983-05-05

申请号：AU5476380

申请日：1980-01-21

Applicant: ABBOTT LAB

Inventor： VOSS HOUSTON FREDERICK ,  PLATTNER JACOB ,  HERRIN THOMAS RAYMOND

IPC: C07F9/40 ,  C12Q1/46 ,  G01N33/532 ,  G01N33/542 ,  C07F9/65 ,  C07G17/00 ,  C07H15/22 ,  C07H23/00 ,  C07J41/00 ,  C07J43/00 ,  C12Q1/00 ,  G01N33/54

Abstract: The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction. The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.