公开(公告)号：EP0353589B1

公开(公告)日：1996-02-07

申请号：EP89113614.5

申请日：1989-07-24

Applicant: ABBOTT LABORATORIES

Inventor： Donohue, Joseph Patrick ,  Mattimiro, Christopher Nicholas ,  Bailey, Mark Wallace

IPC: G01N33/53 ,  G01N21/47 ,  G01N35/02

CPC classification number: G01N35/1009 ,  B01L3/545 ,  G01N21/8483 ,  G01N35/00712 ,  G01N35/00732 ,  G01N2021/478 ,  G01N2035/00752 ,  G01N2035/00762 ,  G01N2035/1025 ,  G01N2035/1086 ,  G01N2201/12753

公开(公告)号：EP0353589A2

公开(公告)日：1990-02-07

申请号：EP89113614.5

申请日：1989-07-24

Applicant: ABBOTT LABORATORIES

Inventor： Donohue, Joseph Patrick ,  Mattimiro, Christopher Nicholas ,  Bailey, Mark Wallace

IPC: G01N33/53 ,  G01N21/47 ,  G01N35/02

CPC classification number: G01N35/1009 ,  B01L3/545 ,  G01N21/8483 ,  G01N35/00712 ,  G01N35/00732 ,  G01N2021/478 ,  G01N2035/00752 ,  G01N2035/00762 ,  G01N2035/1025 ,  G01N2035/1086 ,  G01N2201/12753

Abstract: A semi-automated biological sample analyzer and subsystems are provided to simultaneously perform a plurality of enzyme immuno assays for himan IgE class antibodies specific to a panel of preselected allergens in each of a plurality of biological samples. A carou­sel (18) is provided to position and hold a plurality of reaction cartridges (80). Each reaction cartridge includes a plurality of isolated test sites formed in a two dimensional array in a solid phase binding layer con­tained within a reaction well (86) which is adapted to con­tain a biological sample to be assayed. The carousel and cartridges contain structures which cooperate to precisely position the cartridges in each of three separate dimensions so that each cartridge is posi­tioned uniformly. An optical reader (32) operating on a principle of diffuse reflectance is provided to read the results of the assays from each test site of each cartridge. Also provided is a subsystem which provides predetermined lot-specific assay calibration data which is useful for normalizing the results of various assays with respect to predetermined common standard values.

Abstract translation: 提供半自动化生物样品分析仪和子系统，以同时对多个生物样品中的每一个中预先选择的过敏原的一组特异性的himan IgE类抗体进行多次酶免疫测定。 提供转盘（18）以定位和保持多个反应盒（80）。 每个反应筒包括在包含在反应井（86）内的固相结合层中的二维阵列中形成的多个分离的测试位点，其适于容纳要测定的生物样品。 圆盘传送带和墨盒包含结构，其配合以将墨盒精确地定位在三个分开的尺寸中的每一个中，使得每个墨盒均匀地定位。 提供以漫反射原理工作的光学阅读器（32），以从每个盒的每个测试位置读取测定结果。 还提供了一种子系统，其提供预定的特异性测定校准数据，其可用于相对于预定的共同标准值标准化各种测定的结果。

公开(公告)号：EP0286915A2

公开(公告)日：1988-10-19

申请号：EP88105156.9

申请日：1988-03-30

Applicant: ABBOTT LABORATORIES

Inventor： Bailey, Mark Wallace ,  Ulanday, Myrna Gatan

IPC: G01N33/72

CPC classification number: G01N33/721

Abstract: A reagent system for determining the hemoglobin content of whole blood comprising an aqueous solution of polyvinylpyrrolidone at a pH sufficient to denature hemoglobin in whole blood. A method is also provided wherein a blood sample is treated with an aqueous solution of polyvinylpyrrolidone at a basic pH sufficient to denature the hemoglobin present in the whole blood to form a stable product whereafter the absorbance of the stable product of the blood sample and polyvinylpyrrolidone polymer reagent system is measured at a wavelength of approximately 575 nm to determine the hemoglobin contentration.

Abstract translation: 一种用于测定全血的血红蛋白含量的试剂系统，其包含聚乙烯吡咯烷酮水溶液，其pH足以使全血中的血红蛋白变性。 还提供了一种方法，其中血液样品用聚乙烯吡咯烷酮水溶液处理，碱性pH足以使存在于全血中的血红蛋白变性形成稳定的产物，此后血样和聚乙烯吡咯烷酮聚合物的稳定产物的吸光度 在约575nm的波长处测量试剂系统以确定血红蛋白含量。

公开(公告)号：EP0353591B1

公开(公告)日：1996-04-17

申请号：EP89113616.0

申请日：1989-07-24

Applicant: ABBOTT LABORATORIES

Inventor： Defreese, James Douglas ,  Huang, Tung-Ming ,  Holen, James T. ,  Gray, Robert Carlton ,  Bailey, Mark Wallace ,  Donohue, Joseph Patrick ,  Putterman, Caryn Glickson

IPC: G01N35/02 ,  G01N21/47 ,  G01N33/53

CPC classification number: G01N33/5302 ,  G01J2001/161 ,  G01J2001/1636 ,  G01N21/253 ,  G01N35/00029 ,  G01N2021/0162 ,  G01N2021/478 ,  G01N2035/00099 ,  G01N2035/00752 ,  G01N2035/1025 ,  G01N2201/0415 ,  G01N2201/069 ,  G01N2201/12761

公开(公告)号：EP0353589A3

公开(公告)日：1991-03-13

申请号：EP89113614.5

申请日：1989-07-24

Applicant: ABBOTT LABORATORIES

Inventor： Donohue, Joseph Patrick ,  Mattimiro, Christopher Nicholas ,  Bailey, Mark Wallace

IPC: G01N33/53 ,  G01N21/47 ,  G01N35/02

CPC classification number: G01N35/1009 ,  B01L3/545 ,  G01N21/8483 ,  G01N35/00712 ,  G01N35/00732 ,  G01N2021/478 ,  G01N2035/00752 ,  G01N2035/00762 ,  G01N2035/1025 ,  G01N2035/1086 ,  G01N2201/12753

Abstract: A semi-automated biological sample analyzer and subsystems are provided to simultaneously perform a plurality of enzyme immuno assays for himan IgE class antibodies specific to a panel of preselected allergens in each of a plurality of biological samples. A carou­sel (18) is provided to position and hold a plurality of reaction cartridges (80). Each reaction cartridge includes a plurality of isolated test sites formed in a two dimensional array in a solid phase binding layer con­tained within a reaction well (86) which is adapted to con­tain a biological sample to be assayed. The carousel and cartridges contain structures which cooperate to precisely position the cartridges in each of three separate dimensions so that each cartridge is posi­tioned uniformly. An optical reader (32) operating on a principle of diffuse reflectance is provided to read the results of the assays from each test site of each cartridge. Also provided is a subsystem which provides predetermined lot-specific assay calibration data which is useful for normalizing the results of various assays with respect to predetermined common standard values.

公开(公告)号：EP0353591A2

公开(公告)日：1990-02-07

申请号：EP89113616.0

申请日：1989-07-24

Applicant: ABBOTT LABORATORIES

Inventor： Defreese, James Douglas ,  Huang, Tung-Ming ,  Holen, James T. ,  Gray, Robert Carlton ,  Bailey, Mark Wallace ,  Donohue, Joseph Patrick ,  Putterman, Caryn Glickson

IPC: G01N35/02 ,  G01N21/47 ,  G01N33/53

CPC classification number: G01N33/5302 ,  G01J2001/161 ,  G01J2001/1636 ,  G01N21/253 ,  G01N35/00029 ,  G01N2021/0162 ,  G01N2021/478 ,  G01N2035/00099 ,  G01N2035/00752 ,  G01N2035/1025 ,  G01N2201/0415 ,  G01N2201/069 ,  G01N2201/12761

Abstract: A semi-automated biological sample analyzer and subsystems are provided to simultaneously perform a plurality of enzyme immuno assays for himan IgE class antibodies specific to a panel of preselected allergens in each of a plurality of biological samples. A carou­sel is provided to position and hold a plurality of reaction cartridges. Each reaction cartridge includes a plurality of isolated test sites formed in a two dimensional array in a solid phase binding layer con­tained within a reaction well which is adapted to con­tain a biological sample to be assayed. The carousel and cartridges contain structures which cooperate to precisely position the cartridges in each of three separate dimensions so that each cartridge is posi­tioned uniformly. An optical reader operating on a principle of diffuse reflectance is provided to read the results of the assays from each test site of each cartridge. Also provided is a subsystem which provides predetermined lot-specific assay calibration data which is useful for normalizing the results of various assays with respect to predetermined common standard values.

Abstract translation: 提供半自动化生物样品分析仪和子系统，以同时对多个生物样品中的每一个中预先选择的过敏原的一组特异性的himan IgE类抗体进行多次酶免疫测定。 提供转盘以定位和保持多个反应盒。 每个反应盒包括在包含在反应井内的固相结合层中的二维阵列中形成的多个分离的测试位点，其适于含有要测定的生物样品。 圆盘传送带和墨盒包含结构，其配合以将墨盒精确地定位在三个分开的尺寸中的每一个中，使得每个墨盒均匀地定位。 提供以漫反射原理工作的光学阅读器，以从每个盒的每个测试位置读取测定结果。 还提供了一种子系统，其提供预定的特异性测定校准数据，其可用于相对于预定的共同标准值标准化各种测定的结果。

公开(公告)号：EP0286915B1

公开(公告)日：1993-11-18

申请号：EP88105156.9

申请日：1988-03-30

Applicant: ABBOTT LABORATORIES

Inventor： Bailey, Mark Wallace ,  Ulanday, Myrna Gatan

IPC: G01N33/72

CPC classification number: G01N33/721

公开(公告)号：EP0353591A3

公开(公告)日：1991-03-20

申请号：EP89113616.0

申请日：1989-07-24

Applicant: ABBOTT LABORATORIES

Inventor： Defreese, James Douglas ,  Huang, Tung-Ming ,  Holen, James T. ,  Gray, Robert Carlton ,  Bailey, Mark Wallace ,  Donohue, Joseph Patrick ,  Putterman, Caryn Glickson

IPC: G01N35/02 ,  G01N21/47 ,  G01N33/53

CPC classification number: G01N33/5302 ,  G01J2001/161 ,  G01J2001/1636 ,  G01N21/253 ,  G01N35/00029 ,  G01N2021/0162 ,  G01N2021/478 ,  G01N2035/00099 ,  G01N2035/00752 ,  G01N2035/1025 ,  G01N2201/0415 ,  G01N2201/069 ,  G01N2201/12761

Abstract: A semi-automated biological sample analyzer and subsystems are provided to simultaneously perform a plurality of enzyme immuno assays for himan IgE class antibodies specific to a panel of preselected allergens in each of a plurality of biological samples. A carou­sel is provided to position and hold a plurality of reaction cartridges. Each reaction cartridge includes a plurality of isolated test sites formed in a two dimensional array in a solid phase binding layer con­tained within a reaction well which is adapted to con­tain a biological sample to be assayed. The carousel and cartridges contain structures which cooperate to precisely position the cartridges in each of three separate dimensions so that each cartridge is posi­tioned uniformly. An optical reader operating on a principle of diffuse reflectance is provided to read the results of the assays from each test site of each cartridge. Also provided is a subsystem which provides predetermined lot-specific assay calibration data which is useful for normalizing the results of various assays with respect to predetermined common standard values.

公开(公告)号：EP0286915A3

公开(公告)日：1991-02-27

申请号：EP88105156.9

申请日：1988-03-30

Applicant: ABBOTT LABORATORIES

Inventor： Bailey, Mark Wallace ,  Ulanday, Myrna Gatan

IPC: G01N33/72

CPC classification number: G01N33/721

Abstract: A reagent system for determining the hemoglobin content of whole blood comprising an aqueous solution of polyvinylpyrrolidone at a pH sufficient to denature hemoglobin in whole blood. A method is also provided wherein a blood sample is treated with an aqueous solution of polyvinylpyrrolidone at a basic pH sufficient to denature the hemoglobin present in the whole blood to form a stable product whereafter the absorbance of the stable product of the blood sample and polyvinylpyrrolidone polymer reagent system is measured at a wavelength of approximately 575 nm to determine the hemoglobin contentration.