公开(公告)号：CA2853093A1

公开(公告)日：2013-04-25

申请号：CA2853093

申请日：2012-10-22

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  WU JIAN ,  CHEN YE ,  WANG TIANXING ,  WANG XIAOBO

IPC: G01N15/14 ,  G01N15/00

Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：CA2853093C

公开(公告)日：2020-01-14

申请号：CA2853093

申请日：2012-10-22

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  WU JIAN ,  CHEN YE ,  WANG TIANXING ,  WANG XIAOBO

IPC: G01N15/14 ,  G01N15/00

Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：CA3030966A1

公开(公告)日：2018-01-18

申请号：CA3030966

申请日：2017-07-15

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  KONG LINGBO ,  WU JIAN ,  CHENG TINGTING ,  QING YANGDE ,  WANG XIAOBO

IPC: G01N15/14 ,  G01N21/64

Abstract: An optical engine its use in a bench top flow cytometer, the optical engine having a set of lasers, each focused horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same excitation plane of a flow cell, a set of optics that separate fluorescence of a same wavelength range into different locations in a focal plane of collection optics according to the different lasers by which the fluorescent light is excited; and a detector that selectively detects light from the different locations thereby distinguishing between fluorescence emitted within the same wavelength range as excited by the different lasers.

公开(公告)号：EP2769203A4

公开(公告)日：2015-04-29

申请号：EP12841762

申请日：2012-10-22

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  WU JIAN ,  CHEN YE ,  WANG TIANXING ,  WANG XIAOBO

IPC: G01N21/64 ,  G01N15/14

CPC classification number: G01N21/645 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/149 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/06113 ,  G01N2201/0621

Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

Abstract translation: 一种用于检测由多个激发源，其包括具有两个空间上分开光学询问区的流道所致光的信号成分的系统; 光照明子系统也ausrichtet每个不同波长的两个光束中的至光学询问区的不同的区域; 其将检测到的光转换成具有两个调制和未调制的电信号的总电信号的检测器子系统; 和处理器做bestimmt从来自每个光学询问区检测到的光信号分量。