公开(公告)号：WO2015077349A2

公开(公告)日：2015-05-28

申请号：PCT/US2014066429

申请日：2014-11-19

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  CHEN YE ,  CHUNG HYUNSUN ,  LI ZENGQIANG ,  WANG XIAOBO

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/569

CPC classification number: G01N21/645 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6486 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/06113

Abstract: An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

Abstract translation: 一种用于台式流式细胞仪的光学引擎，该光学引擎包括一组激光器; 用于每个激光器的不同组的光束整形光学器件，其中每个组件包括两个透镜，用于可调节地将光沿x轴水平地聚焦到相同的水平位置，并且沿着y轴沿着同一平面垂直地垂直于不同的垂直位置; 用于从流动池收集荧光的收集光学器件; 过滤光学器件，根据波长范围将从流通池收集的荧光过滤到不同的检测通道; 以及用于将滤波后的荧光转换为电信号的每个检测通道的检测器，其中对电信号进行处理，使得来自不同垂直位置处的每个激光的荧光在相同的检测器处被区分。

公开(公告)号：CA2853093C

公开(公告)日：2020-01-14

申请号：CA2853093

申请日：2012-10-22

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  WU JIAN ,  CHEN YE ,  WANG TIANXING ,  WANG XIAOBO

IPC: G01N15/14 ,  G01N15/00

Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：EP3071951A4

公开(公告)日：2017-08-23

申请号：EP14863825

申请日：2014-11-19

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  CHEN YE ,  CHUNG HYUNSUN ,  LI ZENGQIANG ,  WANG XIAOBO

IPC: G01N21/64 ,  G01N15/10 ,  G01N15/14

CPC classification number: G01N21/645 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6486 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/06113

Abstract: An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

Abstract translation: 一种用于台式流式细胞仪的光学引擎，该光学引擎包括一组激光器; 用于每个激光器的不同组光束成形光学器件，其中每个组包括两个透镜，以沿着x轴将水平可调地聚焦到相同的水平位置，并且沿着y轴垂直地沿着同一平面聚焦到不同的垂直位置; 用于从流动池收集荧光的收集光学器件; 过滤光学器件，其根据波长范围将从流动池收集的荧光过滤到不同的检测通道中; 以及用于每个检测通道的检测器，用于将滤波的荧光转换为电信号，其中处理电信号以使得来自不同垂直位置处的每个激光器的荧光在同一检测器处被区分。

公开(公告)号：EP2769260A4

公开(公告)日：2015-07-29

申请号：EP12845835

申请日：2012-10-19

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  CHEN YE ,  WANG XIAOBO

IPC: G02B27/10

CPC classification number: G02B27/1006 ,  G01J3/14 ,  G01J3/36 ,  G01J3/443 ,  G02B5/04 ,  G02B5/285 ,  G02B27/141 ,  G02B27/142

Abstract: A prism including a substrate faceted to provide a plurality of flat surfaces, wherein at least two of the plurality of surfaces, each including a filter coating, form at least two filter surfaces, wherein each filter surface selectively permits passage of a predetermined wavelength and reflects remaining wavelengths along an optical path towards another of the plurality of surfaces, optionally another filter surface, wherein an angle of incidence of each of the plurality of surfaces along the optical path is equal or nearly equal. An apparatus incorporating the prism and its use for splitting a light spectrum into a plurality of wavelengths or wavelength ranges.

公开(公告)号：CA2853083C

公开(公告)日：2020-03-10

申请号：CA2853083

申请日：2012-10-19

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  CHEN YE ,  WANG XIAOBO

IPC: G02B27/10 ,  G02B5/04

Abstract: A prism including a substrate faceted to provide a plurality of flat surfaces, wherein at least two of the plurality of surfaces, each including a filter coating, form at least two filter surfaces, wherein each filter surface selectively permits passage of a predetermined wavelength and reflects remaining wavelengths along an optical path towards another of the plurality of surfaces, optionally another filter surface, wherein an angle of incidence of each of the plurality of surfaces along the optical path is equal or nearly equal. An apparatus incorporating the prism and its use for splitting a light spectrum into a plurality of wavelengths or wavelength ranges.

公开(公告)号：CA2853093A1

公开(公告)日：2013-04-25

申请号：CA2853093

申请日：2012-10-22

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  WU JIAN ,  CHEN YE ,  WANG TIANXING ,  WANG XIAOBO

IPC: G01N15/14 ,  G01N15/00

Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：EP2769203A4

公开(公告)日：2015-04-29

申请号：EP12841762

申请日：2012-10-22

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  WU JIAN ,  CHEN YE ,  WANG TIANXING ,  WANG XIAOBO

IPC: G01N21/64 ,  G01N15/14

CPC classification number: G01N21/645 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/149 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/06113 ,  G01N2201/0621

Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

Abstract translation: 一种用于检测由多个激发源，其包括具有两个空间上分开光学询问区的流道所致光的信号成分的系统; 光照明子系统也ausrichtet每个不同波长的两个光束中的至光学询问区的不同的区域; 其将检测到的光转换成具有两个调制和未调制的电信号的总电信号的检测器子系统; 和处理器做bestimmt从来自每个光学询问区检测到的光信号分量。

公开(公告)号：CA2853083A1

公开(公告)日：2013-05-10

申请号：CA2853083

申请日：2012-10-19

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  CHEN YE ,  WANG XIAOBO

IPC: G02B27/10 ,  G02B5/04

Abstract: A prism including a substrate faceted to provide a plurality of flat surfaces, wherein at least two of the plurality of surfaces, each including a filter coating, form at least two filter surfaces, wherein each filter surface selectively permits passage of a predetermined wavelength and reflects remaining wavelengths along an optical path towards another of the plurality of surfaces, optionally another filter surface, wherein an angle of incidence of each of the plurality of surfaces along the optical path is equal or nearly equal. An apparatus incorporating the prism and its use for splitting a light spectrum into a plurality of wavelengths or wavelength ranges.