公开(公告)号：CA2438247A1

公开(公告)日：2002-08-29

申请号：CA2438247

申请日：2002-02-19

Applicant: ADVION BIOSCIENCES INC

Inventor： ZHANG SHENG ,  HUANG XIAN

IPC: G01N27/62 ,  B01D15/38 ,  B01J20/281 ,  B01J20/32 ,  B01J45/00 ,  B05B5/08 ,  C12M1/00 ,  C12N15/09 ,  G01N30/26 ,  G01N30/46 ,  G01N30/52 ,  G01N30/56 ,  G01N30/60 ,  G01N30/72 ,  G01N30/88 ,  G01N33/15 ,  H01J49/04 ,  B01D15/08

Abstract: A microchip-based electrospray ionization device and column with affinity adsorbents is disclosed. The invention includes a microchip array.(2) and a capillary tube (1) or alone or attached in combination. At least a portion of the device or column has immobilized affinity adsorbents. Methods for using the device are provided as well for affinity capture of biomolecules to meet the needs for the modern life sciences such as proteomics and drug discover.

公开(公告)号：AU6977301A

公开(公告)日：2001-12-17

申请号：AU6977301

申请日：2001-06-11

Applicant: ADVION BIOSCIENCES INC

Inventor： HUANG XIAN

IPC: G01N30/26 ,  B01D15/08 ,  B01J20/26 ,  B01J20/281 ,  B01J20/285 ,  B01J20/287 ,  B01J20/32 ,  C08J9/36 ,  G01N30/34 ,  G01N30/72 ,  G01N30/88

公开(公告)号：WO0153819A9

公开(公告)日：2002-10-17

申请号：PCT/US0101785

申请日：2001-01-18

Applicant: ADVION BIOSCIENCES INC

Inventor： CORSO THOMAS N ,  SCHULTZ GARY A ,  PROSSER SIMON J ,  HUANG XIAN

IPC: G01N27/62 ,  B01D15/08 ,  B01D57/02 ,  B01D59/44 ,  B01J20/281 ,  B01J20/283 ,  B01J20/285 ,  B03C5/00 ,  B05B5/043 ,  G01N1/10 ,  G01N21/33 ,  G01N21/53 ,  G01N21/64 ,  G01N27/447 ,  G01N30/28 ,  G01N30/46 ,  G01N30/52 ,  G01N30/60 ,  G01N30/72 ,  G01N30/74 ,  G01N30/88 ,  G01N37/00 ,  H01J49/00 ,  H01J49/04 ,  H01J49/10 ,  G01N27/26 ,  B32B31/00 ,  H01J49/14 ,  G01N27/477

CPC classification number: G01N30/466 ,  B01J20/261 ,  B01J20/267 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/82 ,  G01N27/44717 ,  G01N30/463 ,  G01N30/467 ,  G01N30/48 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

Abstract: A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths (254) in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices (250) can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles (242) for the same fluid for analysis.

Abstract translation: 公开了一种具有分离材料的微制造硅芯片，例如其微通道中原位制备的多孔聚合物整料（254）。 聚合物整料是液体可渗透的，用作液相色谱的微柱，其通过在微通道中含有乙烯基单体和溶剂（致孔剂）的混合物的原位自由基聚合制备。 公开了一种方法和系统，以从一个或多个喷嘴产生一个或多个电喷雾羽流，其提供与质量分析仪测量的离子强度，其大小与为流体中包含的分析形成的电喷雾羽流的数量成比例。 多个电喷射装置（250）可以以小型化的独立电喷雾装置阵列的形式使用，用于从用于分析的相同流体的多个喷嘴（242）产生多个电喷雾羽流。

公开(公告)号：EP1309381A4

公开(公告)日：2003-08-27

申请号：EP01948306

申请日：2001-06-11

Applicant: ADVION BIOSCIENCES INC

Inventor： HUANG XIAN

IPC: G01N30/26 ,  B01D15/08 ,  B01J20/26 ,  B01J20/281 ,  B01J20/285 ,  B01J20/287 ,  B01J20/32 ,  C08J9/36 ,  G01N30/34 ,  G01N30/72 ,  G01N30/88 ,  G01N30/48

CPC classification number: B01J20/287 ,  B01D15/325 ,  B01J20/26 ,  B01J20/261 ,  B01J20/28097 ,  B01J20/285 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/3278 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/82 ,  Y10T428/249987 ,  Y10T428/249988

Abstract: A process is provided for modifying a porous polystyrene monolith to render its internal pore surfaces grafted with alkyl groups, preferably of at least four carbon atoms, which includes the step of alkylating the monolith with a uniform liquid solution containing a Friedel-Crafts catalyst and an alkyl halide. A surface-modified monolith produced thereby is an efficient separation medium in reversed-phase liquid chromatography for both small and large biomolecules.

Abstract translation: 提供了一种改性多孔聚苯乙烯整料以使其内部孔表面接枝有优选至少4个碳原子的烷基的方法，该方法包括用含有弗瑞德 - 克莱福特催化剂的均匀液体溶液烷基化整料的步骤，和 烷基卤。 由此生产的表面改性的整体料在小型和大型生物分子的反相液相色谱中是有效的分离介质。

公开(公告)号：EP1248949A4

公开(公告)日：2007-07-25

申请号：EP01942714

申请日：2001-01-18

Applicant: ADVION BIOSCIENCES INC

Inventor： CORSO THOMAS N ,  SCHULTZ GARY A ,  PROSSER SIMON J ,  HUANG XIAN

IPC: G01N27/62 ,  B01D15/08 ,  B01D57/02 ,  B01D59/44 ,  B01J20/281 ,  B01J20/283 ,  B01J20/285 ,  B03C5/00 ,  B05B5/043 ,  G01N1/10 ,  G01N21/33 ,  G01N21/53 ,  G01N21/64 ,  G01N27/447 ,  G01N30/28 ,  G01N30/46 ,  G01N30/52 ,  G01N30/60 ,  G01N30/72 ,  G01N30/74 ,  G01N30/88 ,  G01N37/00 ,  H01J49/00 ,  H01J49/04 ,  H01J49/10

CPC classification number: G01N30/466 ,  B01J20/261 ,  B01J20/267 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/82 ,  G01N27/44717 ,  G01N30/463 ,  G01N30/467 ,  G01N30/48 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

Abstract: A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths (254) in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices (250) can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles (242) for the same fluid for analysis.

公开(公告)号：EP1363714A4

公开(公告)日：2007-05-09

申请号：EP02717446

申请日：2002-02-19

Applicant: ADVION BIOSCIENCES INC

Inventor： ZHANG SHENG ,  HUANG XIAN

IPC: G01N27/62 ,  G01N30/72 ,  B01D15/08 ,  B01D15/38 ,  B01J20/281 ,  B01J20/32 ,  B01J45/00 ,  B05B5/08 ,  C12M1/00 ,  C12N15/09 ,  G01N30/26 ,  G01N30/46 ,  G01N30/52 ,  G01N30/56 ,  G01N30/60 ,  G01N30/88 ,  G01N33/15 ,  H01J49/04

CPC classification number: G01N30/6095 ,  B01D15/3804 ,  B01J20/28042 ,  B01J20/285 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3244 ,  B01J20/3251 ,  B01J20/3274 ,  B01J45/00 ,  B01J2220/52 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/82 ,  G01N30/463 ,  G01N30/466 ,  G01N30/482 ,  G01N30/7266 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

Abstract: A microchip-based electrospray ionization device and column with affinity adsorbents is disclosed. The invention includes a microchip array.(2) and a capillary tube (1) or alone or attached in combination. At least a portion of the device or column has immobilized affinity adsorbents. Methods for using the device are provided as well for affinity capture of biomolecules to meet the needs for the modern life sciences such as proteomics and drug discover.