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公开(公告)号：JPS63200059A
公开(公告)日：1988-08-18
申请号：JP3370987
申请日：1987-02-17
Applicant: AGENCY IND SCIENCE TECHN
Inventor： SUGATA TAKESHI , SATO SHINJI , SAKO TAKESHI , SAKAKI KEIICHI , NAKAZAWA NOBUAKI , SHIRATA TOSHIKATSU
Abstract: PURPOSE:To portionwise sample a fractionated component at high purity efficiently and continuously without using an adsorbing column and without receiving the restriction of the number of times of fractionation, by providing a change-over valve between a separation column and a low pressure side and portionwise sampling the fractionated component by the operation of the change-over valve. CONSTITUTION:The first flow channel consisting of lines 9, 10 connects a change-over valve 2 and a separation column 1 and the second flow channel composed of a line 12 connects the change-over valve 2 and the separation column 1 through a minute flow rate control valve 7. The third flow channel consisting of lines 14, 15, 24 connects the change-over valve 2 and a low pressure region through a back pressure control valve 8, and the fourth flow channel consisting of lines 16-18, 20-22, 23 connects the change-over valve 2 and the low pressure region through a portionwise sampling change-over valve 3 and portionwise sampling containers a-c. When a supercritical fluid having a mixture to be separated dissolved therein is introduced into the separation column 1, the supercritical fluid wherein the mixture is fractionated into respective components is allowed to flow out from the separation column 1 in a time series manner. Hereupon, the change-over valves 2, 3 are operated in relation to a fractionated component detector 6 and the connection state of each flow channel is altered and each of the fractionated components is portionwise sampled in each of the portionwise sampling containers a-c.
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公开(公告)号：JPS63112536A
公开(公告)日：1988-05-17
申请号：JP25949586
申请日：1986-10-30
Applicant: AGENCY IND SCIENCE TECHN
Inventor： SAKO TAKESHI , YOKOCHI TOSHIHIRO , SATO SHINJI , SUZUKI OSAMU , SHIRATA TOSHIKATSU , SUGATA TAKESHI , NAKAZAWA NOBUAKI
IPC: C07C57/12 , B01D15/08 , C07C51/00 , C07C51/47 , C07C67/00 , C07C67/56 , C07C69/58 , C11C1/08 , G01N30/02
Abstract: PURPOSE:To concentrate and purify gamma-linolenic acid, by subjecting a fatty acid mixture from a natural fat or oil or fatty acid lower alkyl ester mixture to supercritical fluid chromatography. CONSTITUTION:A fatty acid mixture obtained by hydrolyzing a natural fat or oil containing gamma-linolenic acid or fatty acid lower alkyl ester prepared by ester interchange with a lower alcohol is directly fractionated or subjected to urea addition treatment and fractionated by chromatography using a supercritical fluid, e.g. carbon dioxide, flon, etc., in a supercritical state, as a mobile phase to collect a gamma-linolenic acid fraction and carry out concentrating and purifying of the gamma-linolenic acid. The mobile phase is inexpensive and nontoxic without requiring vacuum distillation in the final step and deterioration of yield by thermal denaturation of separation products. A short time is sufficient for the treatment.
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公开(公告)号：JPS6244170A
公开(公告)日：1987-02-26
申请号：JP18148185
申请日：1985-08-19
Applicant: AGENCY IND SCIENCE TECHN
Inventor： SAKO TAKESHI , YOKOCHI TOSHIHIRO , SATO SHINJI , SUZUKI OSAMU , SHIRATA TOSHIKATSU , SUGATA TAKESHI , NAKAZAWA NOBUAKI
Abstract: PURPOSE:To advantageously extract a valuable component contained in a mold of the genus Mortierella, by pretreating the mold by heating followed by grinding and treating it by supercritical fluid or by supercritical fluid using an alcohol and/or a hydrocarbon. CONSTITUTION:A mold of the genus Mortierella is heated and ground by mechanical force, or ground in an alcohol solvent by mechanical force and the mold is pretreated. In the case, the mold is heated at 150-200 deg.C and the mold is pulverized into . Consequently, approximately 100% neutral lipid in the mold can be extracted ad further separation of the lipid from the solvent can be advantageously carried out at a fixed temperature by operation under reduced pressure, etc.
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公开(公告)号：JPS63216845A
公开(公告)日：1988-09-09
申请号：JP5098487
申请日：1987-03-05
Applicant: AGENCY IND SCIENCE TECHN
Inventor： SAKAKI KEIJI , SAKO TAKESHI , SATO SHINJI , SUGATA TAKESHI , SUZUKI OSAMU , NAKAZAWA NOBUAKI , SHIRATA TOSHIKATSU , YOKOCHI TOSHIHIRO
Abstract: PURPOSE:To obtain the titled compound inexpensively, in high yield and harmlessly to human body without requiring removal of organic solvent by vacuum distillation, by fractionating gamma-linolenic acid containing natural fats and oils by chromatography using a supercritical fluid as a mobile phase. CONSTITUTION:gamma-Linolenic acid-containing natural fats and oils are fractionated by chromatography using a supercritical fluid as a mobile phase and a gamma- linolenic acid-containing glyceride fraction is collected to give the aimed com pound. A hydrocarbon such as methane or ethane besides carbon dioxide and freon is used as the supercritical fluid. The supercritical condition is >=72.4kg/cm pressure and >=31.0 deg.C temperature in the case of carbon dioxide. A small amount of ethanol, butane, etc., having 40-120 deg.C boiling point is added to the supercritical fluid so that separating performance is improved. The partial collection condition by the supercritical chromatography is preferably 35-60 deg.C temperature and 90-250kg/cm pressure.
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