公开(公告)号：EP0823475A4

公开(公告)日：2000-10-25

申请号：EP96910187

申请日：1996-04-17

Applicant: AGENCY IND SCIENCE TECHN ,  SUNTORY LTD

Inventor： YOKOCHI TOSHIHIRO ,  NAKAHARA TORO ,  HIGASHIHARA TAKANORI ,  TANAKA SATOHIRO ,  YAGUCHI TOSHIAKI

IPC: A23K1/16 ,  A23K1/18 ,  A23L1/30 ,  A23L1/32 ,  C11B1/00 ,  C11C3/00 ,  C12P7/64 ,  C12N1/14

CPC classification number: C12P7/6427 ,  A23K20/158 ,  A23K50/75 ,  A23K50/80 ,  A23L15/00 ,  A23L33/12 ,  C11B1/00 ,  C11C3/00 ,  C12P7/6472 ,  C12R1/645 ,  Y10S435/911 ,  Y10S435/946

Abstract: The present invention relates to the Schizochytrium genus SR21 strain and a microorganism belonging to the same species as does said SR21 strain or having substantially the same fungological properties as does said SR21 strain, the said SR21 strain and microorganism having the ability to produce the (n-3) series of docosahexaenoic acid (DHA) and the (n-6) series of docosapentaenoic acid (DPA), and the invention also relates to a process for preparing the (n-3) series of DHA and the (n-6) series of DPA utilizing said microorganisms. The microorganisms according to the present invention are superior in their proliferation character and their propensity to produce fat, and have the ability to produce the (n-3) series of DHA and the (n-6) series of DPA very well. Accordingly, it is possible to effectively produce the (n-3) series of DHA and/or the (n-6) series of DPA, which are useful in the fields of foods and pharmaceuticals, using the microorganisms according to the present invention. In addition, the present invention provides a fat obtained by culturing the present microorganisms. Since the fat composition contains the (n-6) series of DPA in addition to the (n-3) series of DHA having various physiological activities, it is possible to stably and effectively supply the (n-6) series of DPA and/or the (n-3) series of DHA to subjects in need of these highly unsaturated fatty acids by adding the fat composition to various feedstuffs or foods.

公开(公告)号：DE3587044T2

公开(公告)日：1993-06-03

申请号：DE3587044

申请日：1985-12-13

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SUZUKI OSAMU ,  YOKOCHI TOSHIHIRO

IPC: C11B1/10 ,  C11B7/00 ,  C12N1/00 ,  C12P7/64

Abstract: PCT No. PCT/JP85/00685 Sec. 371 Date Aug. 22, 1986 Sec. 102(e) Date Aug. 22, 1986 PCT Filed Dec. 13, 1985 PCT Pub. No. WO86/04353 PCT Pub. Date Jul. 31, 1986.The invention relates to a procedure of obtaining a lipid mixture rich in the content of gamma -linolenic acid or glycerides thereof from cultured fungus bodies of a filamentous Mortierella fungus. The invention proposes, on one hand, to fractionally extract the lipid mixture from the fungus bodies under mechanical disintegration in two steps first with an alcohol containing water to give a fraction rich in the polar lipids and then with a hydrocarbon solvent, e.g. hexane, to give a fraction lean in the polar lipids. The invention proposes, on the other hand, to enrich a lipid mixture relative to gamma -linolenic acid by fractionally crystallizing a part of a starting lipid mixture in a solution of a specified solvent so that the uncrystallized fraction in the mother liquor contains a lipid mixture in which the content of gamma -linolenic acid is remarkably increased in comparison with the starting lipid mixture.

公开(公告)号：DE3470061D1

公开(公告)日：1988-04-28

申请号：DE3470061

申请日：1984-09-25

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SUZUKI OSAMU ,  YOKOCHI TOSHIHIRO

IPC: C12N1/14 ,  C12P7/64

Abstract: A very efficient microbiological method is proposed for the preparation of gamma -linolenic acid or a lipid rich in the content of gamma -linolenic acid, one of the essential fatty acids in the diet of mammals, e.g. human. The method comprises culturing a fungus of Mortierella genus including isabellina, vinacea, ramanniana, ramanniana var. angulispora and nana in a culture medium containing a carbohydrate, e.g. glucose, as the carbon source in an unusually high concentration of 60 to 400 g/liter to grow a fungal body containing a large amount of a lipid rich in the content of gamma -linolenic acid. A further improvement in the efficiency of culture and the content of the desired fatty acid in the fungal body is obtained by the addition of acetic acid or an alkali acetate to the culture medium.

公开(公告)号：DE3587044D1

公开(公告)日：1993-03-11

申请号：DE3587044

申请日：1985-12-13

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SUZUKI OSAMU ,  YOKOCHI TOSHIHIRO

IPC: C11B1/10 ,  C11B7/00 ,  C12N1/00 ,  C12P7/64

Abstract: PCT No. PCT/JP85/00685 Sec. 371 Date Aug. 22, 1986 Sec. 102(e) Date Aug. 22, 1986 PCT Filed Dec. 13, 1985 PCT Pub. No. WO86/04353 PCT Pub. Date Jul. 31, 1986.The invention relates to a procedure of obtaining a lipid mixture rich in the content of gamma -linolenic acid or glycerides thereof from cultured fungus bodies of a filamentous Mortierella fungus. The invention proposes, on one hand, to fractionally extract the lipid mixture from the fungus bodies under mechanical disintegration in two steps first with an alcohol containing water to give a fraction rich in the polar lipids and then with a hydrocarbon solvent, e.g. hexane, to give a fraction lean in the polar lipids. The invention proposes, on the other hand, to enrich a lipid mixture relative to gamma -linolenic acid by fractionally crystallizing a part of a starting lipid mixture in a solution of a specified solvent so that the uncrystallized fraction in the mother liquor contains a lipid mixture in which the content of gamma -linolenic acid is remarkably increased in comparison with the starting lipid mixture.

公开(公告)号：CA1273640A

公开(公告)日：1990-09-04

申请号：CA499930

申请日：1986-01-20

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SUZUKI OSAMU ,  YOKOCHI TOSHIHIRO

IPC: C11B1/10 ,  C11B7/00 ,  C12N1/00 ,  C12P7/64

Abstract: The invention relates to a procedure of obtaining a lipid mixture rich in the content of .gamma.-linolenic acid or glycerides thereof from a cultured fungus bodies of a filamentous Mortierella fungus. The invention proposes, on one hand, to fractionally extract the lipid mixture from the fungus bodies under mechanical disintegration in two steps first with an alcohol containing water to give a fraction rich in the polar lipids and then with a hydrocarbon solvent, e.g. hexane, to give a fraction lean in the polar lipids. The invention proposes, on the other hand, to enrich a lipid mixture relative to .gamma.-linolenic acid by fractionally crystallizing a part of a staring lipid mixture in a solution of a specified solvent so that the uncrystallized fraction in the mother liquor contains a lipid mixture in which the content of .gamma.-linolenic acid is remarkably increased in comparison with the starting lipid mixture.

公开(公告)号：CA1235083A

公开(公告)日：1988-04-12

申请号：CA473158

申请日：1985-01-30

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SUZUKI OSAMU ,  YOKOCHI TOSHIHIRO

IPC: C12N1/14 ,  C12P7/64

Abstract: A METHOD FOR THE PREPARATION OF A FUNGAL BODY AND A LIPID RICH IN .gamma.-LINOLENIC ACID THEREFROM A very efficient microbiological method is proposed for the preparation of .gamma.-linolenic acid or a lipid rich in the content of .gamma.-linolenic acid as one of the essential fatty acids in the diet of mammals, e.g.human. The method comprises culturing a fungus of Mortierella genus including isabellina, vinacea, ramanniana, ramanniana var. angulispora and nana in a culture medium containing a carbohydrate, e.g. glucose, as the carbon source in an unusually high concentration of 60 to 400 g/liter to grow the fungal body containing a large amount of a lipid rich in the content of .gamma.-linolenic acid. A further improvement in the efficiency of culture and the content of the desired fatty acid in the fungal body is obtained by the addition of acetic acid or an alkali acetate to the culture medium.

公开(公告)号：JPH06113868A

公开(公告)日：1994-04-26

申请号：JP28551892

申请日：1992-09-30

Applicant: AGENCY IND SCIENCE TECHN

Inventor： YAGI HISAAKI ,  YOKOCHI TOSHIHIRO ,  KAMISAKA YASUSHI ,  YAMAOKA MASAKAZU ,  NAKAHARA HARUROU

IPC: C12P7/40 ,  C12R1/38

Abstract: PURPOSE:To efficiently obtain a specific cyclopropanefatty acid using Pseudomonas bacteria. CONSTITUTION:Pseudomonas bacteria are cultured in a medium containing propanol and/or propionic acid or a salt thereof to obtain the objective fatty acid, i.e., 2-pentyl-1-cyclopropane-1-decanoic acid or 2-heptyl-1-cyclopropane-1- octanoic acid.

公开(公告)号：JPH05302087A

公开(公告)日：1993-11-16

申请号：JP13204492

申请日：1992-04-24

Applicant: AGENCY IND SCIENCE TECHN

Inventor： YOKOYAMA SHINYA ,  TSUCHIDE YUTAKA ,  SAWAYAMA SHIGEKI ,  MINOWA TOMOAKI ,  YOKOCHI TOSHIHIRO ,  KAMISAKA YASUSHI ,  NAKAHARA HARUROU ,  SUZUKI OSAMU

IPC: C10G1/00 ,  C10L1/00 ,  C12P1/00

Abstract: PURPOSE:To provide a method by which a cell which accumulates oil or fat is liquefied simply and effectively to give a substance like heavy oil and simultaneously bring the liquefaction product into a state with good phase separation properties. CONSTITUTION:A cell which accumulates oil or fat is kept at high temp. and pressure in the pressence of an aq. medium to thereby be liquefied to give a substance like heavy oil. The conditions of the liquefaction are controlled so that the liquefaction product contg. the substance is brought into a state with good phase separation properties.

公开(公告)号：JPS63112536A

公开(公告)日：1988-05-17

申请号：JP25949586

申请日：1986-10-30

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SAKO TAKESHI ,  YOKOCHI TOSHIHIRO ,  SATO SHINJI ,  SUZUKI OSAMU ,  SHIRATA TOSHIKATSU ,  SUGATA TAKESHI ,  NAKAZAWA NOBUAKI

IPC: C07C57/12 ,  B01D15/08 ,  C07C51/00 ,  C07C51/47 ,  C07C67/00 ,  C07C67/56 ,  C07C69/58 ,  C11C1/08 ,  G01N30/02

Abstract: PURPOSE:To concentrate and purify gamma-linolenic acid, by subjecting a fatty acid mixture from a natural fat or oil or fatty acid lower alkyl ester mixture to supercritical fluid chromatography. CONSTITUTION:A fatty acid mixture obtained by hydrolyzing a natural fat or oil containing gamma-linolenic acid or fatty acid lower alkyl ester prepared by ester interchange with a lower alcohol is directly fractionated or subjected to urea addition treatment and fractionated by chromatography using a supercritical fluid, e.g. carbon dioxide, flon, etc., in a supercritical state, as a mobile phase to collect a gamma-linolenic acid fraction and carry out concentrating and purifying of the gamma-linolenic acid. The mobile phase is inexpensive and nontoxic without requiring vacuum distillation in the final step and deterioration of yield by thermal denaturation of separation products. A short time is sufficient for the treatment.

公开(公告)号：JPS6225989A

公开(公告)日：1987-02-03

申请号：JP16530385

申请日：1985-07-26

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SUZUKI OSAMU ,  YOKOCHI TOSHIHIRO

IPC: C12P7/64 ,  C12P13/00 ,  C12R1/645

Abstract: PURPOSE:To make it possible to collect efficiently gamma-linolenic acid, by separating phosphatidylcholine having high content of gamma-linolenic acid from lipid collected from a specific mold and concentrating the phosphatidylcholine. CONSTITUTION:A strain such as Mortierella isabellina, Mortierella vinacea Mortierella ramaniana, Mortierella ramaniana var. anglispora, or Mortierella nana, belonging to the genus Mortierella is cultivated in a medium containing a highly concentrated carbohydrate as a carbon source, a mold with high lipid content is obtained and gamma-linolenic acid is produced from lipid collected from the mold. In producing gamma-linoleic acid, phosphatidylcholine having high content of gamma-linolenic acid is separated from the lipid, concentrated and gamma-linolenic acid is obtained. To separate the phosphatidylcholine, the lipid collected from the mold is subjected to alcohol extraction at the first step of multi-stage extraction.