公开(公告)号：EP1131631B1

公开(公告)日：2009-01-07

申请号：EP99963904.0

申请日：1999-11-16

Applicant: Applied Spectral Imaging Ltd.

Inventor： GARINI, Yuval ,  MCNAMARA, George ,  SOENKSEN, Dirk, G. ,  CABIB, Dario ,  BUCKWALD, Robert, A.

IPC: G01N33/53 ,  C12Q1/54 ,  C12Q1/28 ,  C12Q1/00 ,  C12Q1/42 ,  G01N33/569

CPC classification number: C12Q1/6841 ,  C12Q1/6883 ,  G01J3/02 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J2003/2866 ,  G01N1/30 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N33/53 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6423 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/76 ,  Y10S435/968

Abstract: A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected so that a spectral component associated with each of the N stains is collectible. Figure (1) shows a block diagram illustrating the main components of an imaging spectrometer.

公开(公告)号：EP1131631A1

公开(公告)日：2001-09-12

申请号：EP99963904.0

申请日：1999-11-16

Applicant: Applied Spectral Imaging Ltd.

Inventor： GARINI, Yuval ,  MCNAMARA, George ,  SOENKSEN, Dirk, G. ,  CABIB, Dario ,  BUCKWALD, Robert, A.

IPC: G01N33/53 ,  C12Q1/54 ,  C12Q1/28 ,  C12Q1/00 ,  C12Q1/42

CPC classification number: C12Q1/6841 ,  C12Q1/6883 ,  G01J3/02 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J2003/2866 ,  G01N1/30 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N33/53 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6423 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/76 ,  Y10S435/968

Abstract: A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected so that a spectral component associated with each of the N stains is collectible. Figure (1) shows a block diagram illustrating the main components of an imaging spectrometer.

公开(公告)号：EP0832417B1

公开(公告)日：2003-08-06

申请号：EP96944834.9

申请日：1996-12-10

Applicant: Applied Spectral Imaging Ltd.

Inventor： GARINI, Yuval ,  CABIB, Dario ,  BUCKWALD, Robert, A. ,  SOENKSEN, Dirk, G. ,  KATZIR, Nir ,  WINE, David ,  LAVI, Moshe

IPC: G01J3/28 ,  C12Q1/68

CPC classification number: G01N21/6458 ,  A61B3/12 ,  A61B3/1241 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01B11/0675 ,  G01J3/02 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J3/4535 ,  G01J2003/2866 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/76

公开(公告)号：EP0896631B1

公开(公告)日：2003-03-19

申请号：EP97921198.4

申请日：1997-04-16

Applicant: Applied Spectral Imaging Ltd.

Inventor： GARINI, Yuval ,  CABIB, Dario ,  BUCKWALD, Robert, A. ,  SOENKSEN, Dirk, G. ,  RIED, Thomas

IPC: C12Q1/68 ,  G02B21/00 ,  G01J3/28

CPC classification number: G01N21/6452 ,  C12Q1/6841 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01J3/02 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J2003/2866 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/76 ,  C12Q2563/107 ,  C12Q2537/143

公开(公告)号：EP1042727A1

公开(公告)日：2000-10-11

申请号：EP98960520.9

申请日：1998-11-27

Applicant: Applied Spectral Imaging Ltd.

Inventor： SOENKSEN, Dirk, G. ,  MCNAMARA, George ,  GARINI, Yuval ,  KATZIR, Nir

IPC: G06K9/00 ,  G01B9/02

CPC classification number: G01N21/6458 ,  G01B11/0675 ,  G01J3/02 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J5/10 ,  G01J2003/2866 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6423 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/76

Abstract: A method for cancer cell detection including the steps of (a) staining an analyzed sample with at least first and second dyes, the dyes being selected such that the first dye better adheres to normal cells whereas the second dye better adheres to cancer cells; (b) spectrally imaging the sample through an optical device being optically connected to an imaging spectrometer thereby obtaining a spectrum of each pixel of the sample; (c) based on the spectra, evaluating concentrations of the first and second dyes for each of the pixels; and (d) based on the concentrations detecting the presence of cancer cells in the sample.

公开(公告)号：EP0896631A1

公开(公告)日：1999-02-17

申请号：EP97921198.0

申请日：1997-04-16

Applicant: Applied Spectral Imaging Ltd.

Inventor： GARINI, Yuval ,  CABIB, Dario ,  BUCKWALD, Robert, A. ,  SOENKSEN, Dirk, G. ,  RIED, Thomas

IPC: C12N15 ,  C12Q1 ,  G01J3 ,  G01N21 ,  G01N33 ,  G02B21 ,  G06K9

CPC classification number: G01N21/6452 ,  C12Q1/6841 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01J3/02 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J2003/2866 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/76 ,  C12Q2563/107 ,  C12Q2537/143

Abstract: A spectral imaging method for simultaneous detection of multiple fluorophores aimed at detecting and analyzing fluorescent in situ hybridizations employing numerous chromosome paints and/or loci specific probes each labeled with a different fluorophore or a combination of fluorophores for color karyotyping, and at multicolor chromosome banding, wherein each chromosome acquires a specifying banding pattern, which pattern is established using groups of chromosome fragments labeled with various fluorophores or combinations of fluorophores.