公开(公告)号：EP0920537A1

公开(公告)日：1999-06-09

申请号：EP98913120.0

申请日：1998-03-27

Applicant: Applied Spectral Imaging Ltd.

Inventor： GARINI, Yuval ,  KATZIR, Nir ,  WINE, David ,  CABIB, Dario

IPC: G01N33 ,  C12M1 ,  C12M3 ,  C12N15 ,  C12Q1 ,  G01J3 ,  G01N1 ,  G01N21 ,  G01N37 ,  G02B21 ,  G06K9

CPC classification number: G01J3/12 ,  C12Q1/6816 ,  G01J3/02 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J2003/2866 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6423 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/0014 ,  G06K9/76

Abstract: A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) using the pixels belonging to the other L-K chromosomes or portions of chromosomes for calculating the other L-K internal reference vectors, thereby finding all of the L internal reference vectors. A method for classification of L chromosomes or portions of chromosomes of a cell similarly painted using the above method for finding L internal reference vectors, and using the L reference vectors for classification of each of the pixels into one of L classification classes. And, images presenting color chromosomes.

公开(公告)号：EP0832417B1

公开(公告)日：2003-08-06

申请号：EP96944834.9

申请日：1996-12-10

Applicant: Applied Spectral Imaging Ltd.

Inventor： GARINI, Yuval ,  CABIB, Dario ,  BUCKWALD, Robert, A. ,  SOENKSEN, Dirk, G. ,  KATZIR, Nir ,  WINE, David ,  LAVI, Moshe

IPC: G01J3/28 ,  C12Q1/68

CPC classification number: G01N21/6458 ,  A61B3/12 ,  A61B3/1241 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01B11/0675 ,  G01J3/02 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J3/4535 ,  G01J2003/2866 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/76

公开(公告)号：EP1107688A1

公开(公告)日：2001-06-20

申请号：EP99940981.6

申请日：1999-08-16

Applicant: Applied Spectral Imaging Ltd.

Inventor： ADEL, Michael, E. ,  CABIB, Dario ,  WINE, David

IPC: A61B3/10

CPC classification number: A61B3/10 ,  G01J3/10 ,  G01J3/2823 ,  G01J2003/1282

Abstract: This invention is a device, and method for spectral imaging of an object. A plurality of sets of narrow-band light sources (12) such as LEDs are provided. Each set emits illumination radiation in a different narrow spectral band. Each set is activated sequentially to illuminate the object (16). Light reflected from the object or transmitted by the object is focused (26) on a detector array (20) to image the object. Narrower illumination bands are provided by dispersing the emitted light using a dispersive optical element such as a diffraction grating. Alternatively, selected sets or subsets are activated simultaneously with duty cycles that emulate a preselected spectral distribution. For imaging ocular fundus tissue, the illumination light is shaped into an annular beam by an appropriately shaped wave-guide.