公开(公告)号：CA3149293A1

公开(公告)日：2010-04-01

申请号：CA3149293

申请日：2009-09-23

Applicant: BIO RAD LABORATORIES

Inventor： COLSTON BILLY WAYNE ,  HINDSON BENJAMIN JOSEPH ,  NESS KEVIN DEAN ,  MASQUELIER DONALD ARTHUR ,  MILANOVICH FRED PAUL ,  MODLIN DOUGLAS N ,  RIOT VINCENT ,  BURD SAMUEL ,  MAKAREWICZ ANTHONY JOSEPH ,  BELGRADER PHILLIP

Abstract: A method of analysis which involves selecting a device having a port connected to a chamber and placing a sample-containing fluid into the port. The method also involves creating a pressure differential that drives the sample-containing fluid from the port to the chamber and separates the sample-containing fluid into droplets, forming a two-dimensional monolayer of the droplets in the chamber, and imaging at least a portion of the monolayer.

公开(公告)号：CA3075139A1

公开(公告)日：2010-04-01

申请号：CA3075139

申请日：2009-09-23

Applicant: BIO RAD LABORATORIES

Inventor： COLSTON BILLY WAYNE ,  HINDSON BENJAMIN JOSEPH ,  NESS KEVIN DEAN ,  MASQUELIER DONALD ARTHUR ,  MILANOVICH FRED PAUL ,  MODLIN DOUGLAS N ,  RIOT VINCENT ,  BURD SAMUEL ,  MAKAREWICZ ANTHONY JOSEPH ,  BELGRADER PHILLIP

IPC: G01N33/48 ,  G01N31/20 ,  G01N33/52 ,  G01N35/02

Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.

公开(公告)号：CA3075139C

公开(公告)日：2022-04-12

申请号：CA3075139

申请日：2009-09-23

Applicant: BIO RAD LABORATORIES

Inventor： COLSTON BILLY WAYNE ,  HINDSON BENJAMIN JOSEPH ,  NESS KEVIN DEAN ,  MASQUELIER DONALD ARTHUR ,  MILANOVICH FRED PAUL ,  MODLIN DOUGLAS N ,  RIOT VINCENT ,  BURD SAMUEL ,  MAKAREWICZ ANTHONY JOSEPH ,  BELGRADER PHILLIP

IPC: G01N33/48 ,  G01N31/20 ,  G01N33/52 ,  G01N35/02

Abstract: A method of performing a droplet-based assay involving obtaining droplets encapsulated by an immiscible liquid and packed closely together in a monolayer. The method also involves performing a reaction in the droplets while packed closely together in the monolayer, and collecting data related to an analyte from a plurality of the droplets while the droplets remain closely packed together in the monolayer.