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公开(公告)号：CA3041540A1
公开(公告)日：2011-03-24
申请号：CA3041540
申请日：2010-09-20
Applicant: BIO RAD LABORATORIES , AKONNI BIOSYSTEMS
Inventor： BELGRADER PHILLIP , HINDSON BENJAMIN
IPC: C12N1/06 , B01F7/16 , B01F13/08 , C12M1/33 , C12Q1/6806
Abstract: A Method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
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公开(公告)号：CA3149293A1
公开(公告)日：2010-04-01
申请号：CA3149293
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ ANTHONY JOSEPH , BELGRADER PHILLIP
Abstract: A method of analysis which involves selecting a device having a port connected to a chamber and placing a sample-containing fluid into the port. The method also involves creating a pressure differential that drives the sample-containing fluid from the port to the chamber and separates the sample-containing fluid into droplets, forming a two-dimensional monolayer of the droplets in the chamber, and imaging at least a portion of the monolayer.
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Patent Agency Ranking
公开(公告)号：CA3041540C
公开(公告)日：2021-08-24
申请号：CA3041540
申请日：2010-09-20
Applicant: BIO RAD LABORATORIES , AKONNI BIOSYSTEMS
Inventor： BELGRADER PHILLIP , HINDSON BENJAMIN
IPC: C12N1/06 , B01F7/16 , B01F13/08 , C12M1/33 , C12Q1/6806
Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein, The container is dimensioned to allow rotation of the magnetic stir element inside the container.
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公开(公告)号：CA3075139C
公开(公告)日：2022-04-12
申请号：CA3075139
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ ANTHONY JOSEPH , BELGRADER PHILLIP
Abstract: A method of performing a droplet-based assay involving obtaining droplets encapsulated by an immiscible liquid and packed closely together in a monolayer. The method also involves performing a reaction in the droplets while packed closely together in the monolayer, and collecting data related to an analyte from a plurality of the droplets while the droplets remain closely packed together in the monolayer.
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公开(公告)号：ES2709123T3
公开(公告)日：2019-04-15
申请号：ES16166269
申请日：2010-09-20
Applicant: AKONNI BIOSYSTEMS , BIO RAD LABORATORIES
Inventor： BELGRADER PHILLIP , HINDSON BENJAMIN
Abstract: Un método para lisar una o más células, que comprende: colocar una pluralidad de cámaras en una superficie, donde cada cámara comprende una o más células de interés, uno o más elementos de agitación magnética, y una pluralidad de perlas; y rotar un imán entre 1000 y 6000 rpm durante 90 a 120 segundos alrededor de un eje para producir un campo magnético de rotación, donde el eje es paralelo a la superficie sobre la cual residen las cámaras, la rotación del imán hace que uno o más agitadores magnéticos en las cámaras roten, lo que a su vez dirige el movimiento de las perlas, una o más células y el medio líquido y lisa las células dentro de las cámaras.
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公开(公告)号：AU2009297108B2
公开(公告)日：2015-02-12
申请号：AU2009297108
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON JR BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ JR ANTHONY JOSEPH , BELGRADER PHILLIP
IPC: G01N21/00
Abstract: Systems, including apparatus and methods, for performing assays. These systems may involve separating sample components by partitioning them into droplets or other partitions, amplifying or otherwise reacting the components within the droplets, detecting the amplified components, or characteristics thereof, and/or analyzing the resulting data, among others.
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公开(公告)号：CA3075139A1
公开(公告)日：2010-04-01
申请号：CA3075139
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ ANTHONY JOSEPH , BELGRADER PHILLIP
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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