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公开(公告)号：GB2502409B8
公开(公告)日：2014-10-15
申请号：GB201303939
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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公开(公告)号：GB2504241B
公开(公告)日：2014-03-12
申请号：GB201319376
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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公开(公告)号：CA3149293A1
公开(公告)日：2010-04-01
申请号：CA3149293
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ ANTHONY JOSEPH , BELGRADER PHILLIP
Abstract: A method of analysis which involves selecting a device having a port connected to a chamber and placing a sample-containing fluid into the port. The method also involves creating a pressure differential that drives the sample-containing fluid from the port to the chamber and separates the sample-containing fluid into droplets, forming a two-dimensional monolayer of the droplets in the chamber, and imaging at least a portion of the monolayer.
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公开(公告)号：CA3075139C
公开(公告)日：2022-04-12
申请号：CA3075139
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ ANTHONY JOSEPH , BELGRADER PHILLIP
Abstract: A method of performing a droplet-based assay involving obtaining droplets encapsulated by an immiscible liquid and packed closely together in a monolayer. The method also involves performing a reaction in the droplets while packed closely together in the monolayer, and collecting data related to an analyte from a plurality of the droplets while the droplets remain closely packed together in the monolayer.
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公开(公告)号：GB2502409B
公开(公告)日：2014-03-12
申请号：GB201303939
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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Patent Agency Ranking
公开(公告)号：GB2504241A
公开(公告)日：2014-01-22
申请号：GB201319376
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A droplet based assay comprising an emulsion including droplets packed in a three-dimensional arrangement, the droplets are reacted, such as thermocycled in this packaged form and then passed serially through a detector region to analyse individual droplets. Other inventions relate to formation of a plurality of emsulsions together and collecting data from individual droplets, these assays may be specific methods of nucleic acid amplification which include thermocycling and that may be conducted from a multiwall plate array of samples, a droplet transporter may pick-up droplets from a sample holder .
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公开(公告)号：GB2502409A
公开(公告)日：2013-11-27
申请号：GB201303939
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A droplet based assay comprising an emulsion including droplets packed in a three-dimensional arrangement, the droplets are reacted, such as thermocycled in this packaged form and then passed serially through a detector region to analyse individual droplets. Other inventions relate to formation of a plurality of emsulsions together and collecting data from individual droplets, these assays may be specific methods of nucleic acid amplification which include thermocycling and that may be conducted from a multiwall plate array of samples, a droplet transporter may pick-up droplets from a sample holder .
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公开(公告)号：AU2009297108B2
公开(公告)日：2015-02-12
申请号：AU2009297108
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON JR BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ JR ANTHONY JOSEPH , BELGRADER PHILLIP
IPC: G01N21/00
Abstract: Systems, including apparatus and methods, for performing assays. These systems may involve separating sample components by partitioning them into droplets or other partitions, amplifying or otherwise reacting the components within the droplets, detecting the amplified components, or characteristics thereof, and/or analyzing the resulting data, among others.
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公开(公告)号：GB2504241B8
公开(公告)日：2014-10-15
申请号：GB201319376
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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公开(公告)号：GB2504241A8
公开(公告)日：2014-10-15
申请号：GB201319376
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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