公开(公告)号：AU2002344839B2

公开(公告)日：2006-05-11

申请号：AU2002344839

申请日：2002-06-19

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HAMID R ,  GINGRICH JEFFREY ,  KOTCHOU KEITH ,  BHATT NEERAJ

IPC: G01N21/64 ,  G01N27/26 ,  G01N21/27 ,  G01N27/447

Abstract: The optical imaging of two-dimensional solute zone arrays in electrophoresis gels is corrected for nonuniformities in the optical system such as those arising from the light source or from light dispersion underneath the gel. The correction is achieved by the use of a reference plate that responds to a light source uniformly along its length and width by being either uniformly light absorptive or uniformly light transmissive, or by emitting light upon excitation. Thus, any nonuniformities or deviations in the image of the reference plate arise only from nonuniformities or deviations within the optical system. Analogous corrections are made in other two-dimensional assay images, such as microarrays and microtiter plates.

公开(公告)号：CA2450385C

公开(公告)日：2007-11-13

申请号：CA2450385

申请日：2002-06-19

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HAMID R ,  BHATT NEERAJ ,  KOTCHOU KEITH ,  GINGRICH JEFFREY

IPC: G01N21/64 ,  G01N21/93 ,  G01N21/27 ,  G01N27/447 ,  G01N33/483

Abstract: The optical imaging of two-dimensional solute zone arrays in electrophoresis gels is corrected for nonuniformities in the optical system such as those arising from the light source or from light dispersion underneath the gel. T he correction is achieved by the use of a reference plate that responds to a light source uniformly along its length and width by being either uniformly light absorptive or uniformly light transmissive, or by emitting light upon excitation. Thus, any nonuniformities or deviations in the image of the reference plate arise only from nonuniformities or deviations within the optical system. Analogous corrections are made in other two-dimensional assa y images, such as microarrays and microtiter plates.

公开(公告)号：CA2763369A1

公开(公告)日：2010-12-09

申请号：CA2763369

申请日：2010-06-01

Applicant: BIO RAD LABORATORIES

Inventor： KOTCHOU KEITH ,  SCHULTZ CHRISTOF ,  MCDONALD KEVIN ,  BHATT NEERAJ

IPC: G06K9/00

Abstract: Methods, apparatuses, and systems for imaging biological/chemical samples are provided. A calibrated imaging system can allow a user to obtain an optimal focus setting (position) for any effective distance (e.g. a zoom setting). The optimal focus can be determined from a functional approximation that defines a relationship between effective distance and focus setting. A user can input a size, and an imaging system can determine the appropriate effective distance and focus. An imaging system can also determine a size based on any effective distance. A flat-field correction can also be determined for any effective distance or size.

公开(公告)号：CA2450385A1

公开(公告)日：2003-01-09

申请号：CA2450385

申请日：2002-06-19

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HAMID R ,  KOTCHOU KEITH ,  BHATT NEERAJ ,  GINGRICH JEFFREY

IPC: G01N21/64 ,  G01N21/27 ,  G01N27/447 ,  G01N27/26

Abstract: The optical imaging of two-dimensional solute zone arrays in electrophoresis gels is corrected for nonuniformities in the optical system such as those arising from the light source or from light dispersion underneath the gel. T he correction is achieved by the use of a reference plate that responds to a light source uniformly along its length and width by being either uniformly light absorptive or uniformly light transmissive, or by emitting light upon excitation. Thus, any nonuniformities or deviations in the image of the reference plate arise only from nonuniformities or deviations within the optical system. Analogous corrections are made in other two-dimensional assa y images, such as microarrays and microtiter plates.

公开(公告)号：EP2438554A4

公开(公告)日：2015-07-29

申请号：EP10783936

申请日：2010-06-01

Applicant: BIO RAD LABORATORIES

Inventor： KOTCHOU KEITH ,  SCHULTZ CHRISTOF ,  MCDONALD KEVIN ,  BHATT NEERAJ

IPC: G06T7/00

CPC classification number: G06T7/0018 ,  G06T7/80 ,  G06T2207/30024 ,  G06T2207/30208

Abstract: Methods, apparatuses, and systems for imaging biological/chemical samples are provided. A calibrated imaging system can allow a user to obtain an optimal focus setting (position) for any effective distance (e.g. a zoom setting). The optimal focus can be determined from a functional approximation that defines a relationship between effective distance and focus setting. A user can input a size, and an imaging system can determine the appropriate effective distance and focus. An imaging system can also determine a size based on any effective distance. A flat-field correction can also be determined for any effective distance or size.

公开(公告)号：EP2825867A4

公开(公告)日：2016-02-17

申请号：EP13761630

申请日：2013-03-14

Applicant: BIO RAD LABORATORIES

Inventor： KOTCHOU KEITH ,  MCDONALD KEVIN ,  LEE JAMES

IPC: G01N21/76

CPC classification number: G01N21/76

Abstract: An image system for detecting chemiluminescence in a sample uses a highly binned, short exposure initial image to calculate the exposure time for a final image of the sample. After calculation of the exposure time, at least two final images are taken, with saturated pixels removed and replaced in a first image with corresponding unsaturated pixels from a second image. The corresponding pixels are adjusted to reflect the different intensity levels between the first and second images, and the first image becomes the final image reflecting the detected chemiluminescence.

公开(公告)号：EP1410005A4

公开(公告)日：2007-07-11

申请号：EP02744465

申请日：2002-06-19

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HAMID R ,  KOTCHOU KEITH ,  BHATT NEERAJ ,  GINGRICH JEFFREY

IPC: G01N21/64 ,  G01N21/27 ,  G01N27/447

CPC classification number: G01N27/44721 ,  G01N21/276

Abstract: The optical imaging of two-dimensional solute zone arrays in electrophoresis gels is corrected for nonuniformities in the optical system such as those arising from the light source or from light dispersion underneath the gel. The correction is achieved by the use of a reference plate that responds to a light source uniformly along its length and width by being either uniformly light absorptive or uniformly light transmissive, or by emitting light upon excitation. Thus, any nonuniformities or deviations in the image of the reference plate arise only from nonuniformities or deviations within the optical system. Analogous corrections are made in other two-dimensional assay images, such as microarrays and microtiter plates.