公开(公告)号：WO2014145582A2

公开(公告)日：2014-09-18

申请号：PCT/US2014030374

申请日：2014-03-17

Applicant: BIO RAD LABORATORIES ,  CHIA ERIN R ,  HIDDESSEN AMY L ,  HINDSON BENJAMIN J ,  LOWE ADAM ,  HAN CHUNXIAO

Inventor： CHIA ERIN R ,  HIDDESSEN AMY L ,  HINDSON BENJAMIN J ,  LOWE ADAM ,  HAN CHUNXIAO

IPC: C12P7/64

CPC classification number: C07F7/184 ,  C08G77/46 ,  C08G77/50 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

Abstract translation: 用于制备和使用包括硅油和硅氧烷表面活性剂的乳液的方法和组合物的系统。 乳液可以包括设置在包括硅油和硅氧烷表面活性剂的连续相中的水性液滴。 水滴可以含有任选地部分占据的分析物和/或发光（例如光致发光）报告物。 分析物的测定可以用液滴进行。 在一些情况下，可以从液滴中检测信号，并且可以基于信号来确定分析物的特征，例如分析物水平或活性。

公开(公告)号：SG11201507087WA

公开(公告)日：2015-10-29

申请号：SG11201507087W

申请日：2014-03-07

Applicant: BIO RAD LABORATORIES

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

IPC: C12Q1/68 ,  G01N33/52

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：AU2014225372A1

公开(公告)日：2015-09-24

申请号：AU2014225372

申请日：2014-03-07

Applicant: BIO RAD LABORATORIES

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

IPC: C12Q1/68 ,  G01N33/52

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：AU2014225372B2

公开(公告)日：2019-10-24

申请号：AU2014225372

申请日：2014-03-07

Applicant: BIO RAD LABORATORIES

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

IPC: C12Q1/68 ,  G01N33/52

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：SG10201710049RA

公开(公告)日：2018-01-30

申请号：SG10201710049R

申请日：2014-03-07

Applicant: BIO-RAD LABORATORIES INC

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：EP2970668A4

公开(公告)日：2016-08-10

申请号：EP14762805

申请日：2014-03-17

Applicant: BIO RAD LABORATORIES ,  CHIA ERIN R ,  HIDDESSEN AMY L ,  HINDSON BENJAMIN J ,  LOWE ADAM ,  HAN CHUNXIAO

Inventor： CHIA ERIN R ,  HIDDESSEN AMY L ,  HINDSON BENJAMIN J ,  LOWE ADAM ,  HAN CHUNXIAO

IPC: G01N33/50 ,  C08G77/46 ,  C08G77/50 ,  C08L83/12 ,  C08L83/14

CPC classification number: C07F7/184 ,  C08G77/46 ,  C08G77/50 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

Abstract translation: 用于制备和使用包含硅油和硅氧烷表面活性剂的乳液的方法和组合物的系统。 乳液可以包括设置在包括硅油和硅氧烷表面活性剂的连续相中的水性液滴。 含水液滴可以含有任选地部分占据的分析物和/或发光（例如光致发光）报告物。 分析物的测定可以用液滴进行。 在一些情况下，可以从液滴中检测信号，并且可以基于这些信号来确定分析物的特征，例如分析物水平或活性。

公开(公告)号：EP2964787A4

公开(公告)日：2016-08-17

申请号：EP14759550

申请日：2014-03-07

Applicant: BIO RAD LABORATORIES

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

IPC: C12Q1/68 ,  G01N33/52

CPC classification number: C12Q1/6804 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q2527/125 ,  C12Q2563/159 ,  C12Q2537/143

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

Abstract translation: 本公开提供了用于在聚合酶链反应测定中检测核酸的方法，装置，系统和组合物，例如液滴数字聚合酶链反应（ddPCR）测定。 本公开提供了使用插层染料在ddPCR测定中检测核酸的方法，装置，系统和组合物。 具有至少一种含氟表面活性剂和至少一种非离子非氟表面活性剂的双表面活性剂体系可用于液滴产生和核酸检测。