公开(公告)号：AU2014225372B2

公开(公告)日：2019-10-24

申请号：AU2014225372

申请日：2014-03-07

Applicant: BIO RAD LABORATORIES

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

IPC: C12Q1/68 ,  G01N33/52

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：SG10201710049RA

公开(公告)日：2018-01-30

申请号：SG10201710049R

申请日：2014-03-07

Applicant: BIO-RAD LABORATORIES INC

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：SG11201507087WA

公开(公告)日：2015-10-29

申请号：SG11201507087W

申请日：2014-03-07

Applicant: BIO RAD LABORATORIES

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

IPC: C12Q1/68 ,  G01N33/52

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：AU2014225372A1

公开(公告)日：2015-09-24

申请号：AU2014225372

申请日：2014-03-07

Applicant: BIO RAD LABORATORIES

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

IPC: C12Q1/68 ,  G01N33/52

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

公开(公告)号：EP2964787A4

公开(公告)日：2016-08-17

申请号：EP14759550

申请日：2014-03-07

Applicant: BIO RAD LABORATORIES

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

IPC: C12Q1/68 ,  G01N33/52

CPC classification number: C12Q1/6804 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q2527/125 ,  C12Q2563/159 ,  C12Q2537/143

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

Abstract translation: 本公开提供了用于在聚合酶链反应测定中检测核酸的方法，装置，系统和组合物，例如液滴数字聚合酶链反应（ddPCR）测定。 本公开提供了使用插层染料在ddPCR测定中检测核酸的方法，装置，系统和组合物。 具有至少一种含氟表面活性剂和至少一种非离子非氟表面活性剂的双表面活性剂体系可用于液滴产生和核酸检测。

公开(公告)号：EP2951320A4

公开(公告)日：2016-08-10

申请号：EP14746304

申请日：2014-02-03

Applicant: BIO RAD LABORATORIES

Inventor： REGAN JOHN F ,  TZONEV SVILEN S ,  JOUVENOT YANN ,  LITTERST CLAUDIA ,  MAAR DIANNA ,  MCDERMOTT GEOFF

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6851 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2545/107

Abstract: Digital assay system, including methods and apparatus, for calculating a level of one or more targets. In an exemplary method, data for amplification of a plurality of targets including a first target may be collected from partitions. A level of the first target may be calculated from only a subset of the data that excludes partitions according to target content.