公开(公告)号：CA2832759A1

公开(公告)日：2012-11-15

申请号：CA2832759

申请日：2012-05-01

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN ,  CHU DANIEL Y ,  PATT PAUL J ,  SADRI AMIR ,  TONG ROGER

IPC: B01L7/00

Abstract: Rapid and uniform temperature changes in the wells of a microplate or any thin-walled plate that contains an array of reaction wells or sample receptacles are achieved by the use of heating and cooling elements with a vapor chamber interposed between such elements and the microplate. The upper surface of the vapor chamber and the underside of the sample plate in certain embodiments are complementary in shape, i.e., they have identical but oppositely directed contours in the areas around each of the sample receptacles, to provide continuous surface contact along the surface of each receptacle. In other embodiments, an intermediary plate is placed between the vapor chamber and the well plate, with the top surface of the intermediary plate being complementary in shape to the underside of the well plate.

公开(公告)号：EP2704835A4

公开(公告)日：2014-12-24

申请号：EP12782793

申请日：2012-05-01

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN ,  CHU DANIEL Y ,  PATT PAUL J ,  SADRI AMIR ,  TONG ROGER

IPC: B01L7/00

CPC classification number: B01L7/52 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1855

公开(公告)号：EP3183556A4

公开(公告)日：2018-06-06

申请号：EP15834450

申请日：2015-08-19

Applicant: BIO RAD LABORATORIES

Inventor： CHU DANIEL Y ,  PATT PAUL J ,  TONG ROGER

IPC: G01N15/14 ,  B01L3/00 ,  C12Q1/04 ,  G01N15/10 ,  G01N21/31

CPC classification number: G01N15/1404 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0424 ,  B01L2400/0454 ,  C12Q1/04 ,  G01N2015/1006 ,  G01N2015/1415

Abstract: A method is provided for transporting a plurality of cells through a flow chamber, wherein the cells are initially immobilized on an internal surface of the flow chamber. The method comprises: selectively releasing the cells from the internal surface of the flow chamber; and flowing liquid through the flow chamber such that the released cells travel with the liquid, thereby transporting the cells through the flow chamber. Cells can be immobilized on or selectively released from the internal surface by applying or removing a trapping potential. The trapping potential can arise from an electric field gradient or an optical field gradient. Alternatively, cells can be selectively released from the surface using photocatalysis. Selective release allows cells to be individually observed or analyzed downstream, and can be based on a signal detected from one or more cells immobilized on the surface.