公开(公告)号：WO2005018770A3

公开(公告)日：2005-03-03

申请号：PCT/US2004/026657

申请日：2004-08-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  CITY OF HOPE ,  TAI, Yu-Chong ,  HE, Qing ,  XIE, Jun ,  PANG, Changlin ,  LEE, Terry, D.

Inventor： TAI, Yu-Chong ,  HE, Qing ,  XIE, Jun ,  PANG, Changlin ,  LEE, Terry, D.

IPC: B01D15/08

Abstract: Embodiments in accordance with the present invention relate to packed-column nano-liquid chromatography (nano-LC) systems integrated on-chip, and methods for producing and using same. The microfabricated chip includes a column, frits/filters, an injector, and a detector, fabricated in a process compatible with those conventionally utilized to form integrated circuits. The column can be packed with supports for various different stationary phases to allow performance of different forms of nano-LC, including but not limited to reversed-phase, normal-phase, adsorption, size-exclusion, affinity, and ion chromatography. A cross-channel injector injects a nanolitre/picolitre-volume sample plug at the column inlet. An electrochemical/conductivity sensor integrated at the column outlet measures separation signals. A self-aligned channel-strengthening technique increases pressure rating of the microfluidic system, allowing it to withstand the high pressure normally used in high performance liquid chromatography (HPLC). On-chip sample injection, separation, and detection of mixture of anions in water is successfully demonstrated using ion-exchange nano-LC.

公开(公告)号：WO2005079944A1

公开(公告)日：2005-09-01

申请号：PCT/US2005/005219

申请日：2005-02-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  TAI, Yu-Chong ,  XIE, Jun ,  SHIH, Chi-Yuan ,  HE, Qing

Inventor： TAI, Yu-Chong ,  XIE, Jun ,  SHIH, Chi-Yuan ,  HE, Qing

IPC: B01D15/08

CPC classification number: G01N30/54 ,  G01N30/30 ,  G01N30/6095 ,  G01N2030/3007 ,  G01N2030/3015 ,  G01N2030/303 ,  G01N2030/3053 ,  G01N2035/00108

Abstract: An apparatus for liquid chromatography comprises a liquid chromatography separation column on a substrate, wherein the separation column is coupled to a heater on the substrate. A chip-based temperature controlled liquid chromatography device comprises a substrate, a thermal isolation zone, and a separation column thermally isolated from the substrate by the thermal isolation zone. An apparatus for chip-based liquid chromatography comprising a cooling device is provided. A temperature gradient liquid chromatography system comprises a chip-based temperature controlled liquid chromatography device, a fluidic coupling, and an electrical interface. Methods of making and methods of using of chip-based temperature gradient liquid chromatography devices are also provided.

Abstract translation: 用于液相色谱的装置包括在基底上的液相色谱分离柱，其中分离柱与基底上的加热器相连。 基于芯片的温度控制液相色谱装置包括基底，热隔离区和通过热隔离区与基底热隔离的分离柱。 提供了一种包括冷却装置的基于芯片的液相色谱仪。 温度梯度液相色谱系统包括基于芯片的温度控制液相色谱装置，流体耦合和电接口。 还提供了制备方法和使用基于芯片的温度梯度液相色谱装置的方法。

公开(公告)号：WO2004065290A2

公开(公告)日：2004-08-05

申请号：PCT/US2004/001052

申请日：2004-01-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  XIE, Jun ,  SHIH, Jason ,  TAI, Yu-Chong

Inventor： XIE, Jun ,  SHIH, Jason ,  TAI, Yu-Chong

IPC: B81C

CPC classification number: B81C1/00119 ,  B81B2201/036 ,  B81B2203/0127 ,  B81B2203/0338 ,  F04B43/043 ,  F04B43/082 ,  F04B43/12 ,  Y10T137/87692

Abstract: An electrostatic fluid regulating device and methods. The device has a substrate. The device also has a first electrode coupled to the substrate. The device has a polymer based diaphragm. A second electrode is coupled to the diaphragm. A polymer based fluid chamber is coupled to the diaphragm. The device also has an inlet coupled to the polymer based fluid chamber and an outlet coupled to the polymer based fluid chamber.

Abstract translation: 一种静电流体调节装置及方法。 该装置具有基板。 该器件还具有耦合到衬底的第一电极。 该装置具有基于聚合物的隔膜。 第二电极耦合到隔膜。 基于聚合物的流体室耦合到隔膜。 该装置还具有连接到基于聚合物的流体室的入口和连接到基于聚合物的流体室的出口。

公开(公告)号：WO2004002878A2

公开(公告)日：2004-01-08

申请号：PCT/US2003/020074

申请日：2003-06-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  TAI, Yu-Chong ,  XIE, Jun ,  HE, Qing ,  LEE, Terry, Dwight

Inventor： TAI, Yu-Chong ,  XIE, Jun ,  HE, Qing ,  LEE, Terry, Dwight

IPC: B81C

CPC classification number: G01N30/6095 ,  B01L3/0268 ,  B01L3/50273 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2400/046 ,  F04B17/00 ,  F04B19/006 ,  G01N30/32 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/326 ,  H01J49/04

Abstract: Electrochemical actuation is adopted in an integrated microfluidic chip to transfer fluid for sample preparation, separation and detection. The electrochemical actuation is capable of producing high pressure for on-chip fluidic handling. Technologies and methods are also developed to use only electrical source to control on-chip fluid handling without any external fluidic support. Applications for the devices and methods include micro scale HPLC, ESI-MS, etc.

Abstract translation: 集成微流体芯片采用电化学驱动，用于传送样品制备，分离和检测的流体。 电化学致动能够产生用于片上流体处理的高压。 还开发了技术和方法，仅使用电源来控制片上流体处理，而无需任何外部流体支持。 设备和方法的应用包括微量HPLC，ESI-MS等

公开(公告)号：WO2006113527A2

公开(公告)日：2006-10-26

申请号：PCT/US2006/014239

申请日：2006-04-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  SHIH, Chi-yuan ,  TAI, Yu-Chong ,  XIE, Jun ,  YOUNG, Darron, K. ,  CHEN, Po-Jui

Inventor： SHIH, Chi-yuan ,  TAI, Yu-Chong ,  XIE, Jun ,  YOUNG, Darron, K. ,  CHEN, Po-Jui

IPC: B01D57/00

CPC classification number: B01D15/22 ,  G01N30/02 ,  G01N30/461 ,  G01N30/466 ,  G01N30/6095 ,  G01N30/78 ,  G01N30/96 ,  G01N2030/524 ,  Y10T29/49826 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/362 ,  B01D15/34 ,  B01D15/325 ,  B01D15/361

Abstract: Systems and methods for monitoring analytes in real time using integrated chromatography systems and devices. Integrated microfluidic liquid chromatography devices and systems include multiple separation columns integrated into a single substrate. Using such a device, parallel analysis of multiple samples can be performed simultaneously and/or sequential analysis of a single sample can be performed simultaneously on a single chip or substrate. The devices and systems are well suited for use in high pressure liquid chromatography (HPLC) applications. HPLC chips and devices including embedded parylene channels can be fabricated using a single mask process.

Abstract translation: 使用集成色谱系统和设备实时监测分析物的系统和方法。 集成的微流体液相色谱装置和系统包括集成到单个底物中的多个分离柱。 使用这样的器件，可以同时执行多个样本的并行分析和/或可以在单个芯片或衬底上同时执行单个样本的顺序分析。 这些装置和系统非常适用于高压液相色谱（HPLC）应用。 包括嵌入式聚对二甲苯通道的HPLC芯片和器件可以使用单个掩模工艺来制造。

公开(公告)号：WO2006017217A2

公开(公告)日：2006-02-16

申请号：PCT/US2005/024518

申请日：2005-07-11

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  CITY OF HOPE? ,  TAI, Yu-Chong ,  XIE, Jun ,  SHIH, Jason ,  LEE, Terry ,  MIAO, Yunan

Inventor： TAI, Yu-Chong ,  XIE, Jun ,  SHIH, Jason ,  LEE, Terry ,  MIAO, Yunan

IPC: C23F1/00

CPC classification number: H01J49/04 ,  B01L3/5027 ,  G01N30/6095 ,  G01N30/7233 ,  G01N2030/326

Abstract: A microfluidic system with on-chip pumping which can be used for liquid chromatography and also electrospray ionization mass spectrometry and which provides improved efficiency, better integration with sensors, improved portability, reduced power consumption, and reduced cost. The system can include (A) a main chip comprising: a substrate having a front face and a back face; a chromatography column on the front face of said substrate, wherein said column has an inlet and an outlet; an electrospray ionization (ESI) nozzle on the front face of said substrate, wherein said nozzle has an inlet and an outlet, and wherein the inlet of the nozzle is microfluidically coupled to the outlet of the column; one or more pump systems on the front face of said substrate comprising a pump chamber, one or more electrodes, and an outlet microfluidically coupled to the inlet of said column; and (B) a reservoir chip comprising a front surface and a back surface, wherein the reservoir chip has one or more cavities in the back surface which when disposed next to the front surface of the main chip extends the volume of the pump chamber of one of the pump system. Microfabrication can be used to prepare the chips, which can be assembled with a cover and inserted into a testing jig for electronic control and mass spectral analysis. Peptide separations are demonstrated which compete with present commercial systems.

Abstract translation: 具有片上泵浦的微流体系统，其可用于液相色谱和电喷雾离子化质谱法，其提供更高的效率，更好地与传感器的集成，改进的便携性，降低的功率消耗和降低的成本。 该系统可以包括（A）主芯片，其包括：具有正面和背面的基板; 所述基板前表面上的色谱柱，其中所述色谱柱具有入口和出口; 在所述基板的正面上的电喷雾离子化（ESI）喷嘴，其中所述喷嘴具有入口和出口，并且其中所述喷嘴的入口微流体耦合到所述塔的出口; 所述衬底的前表面上的一个或多个泵系统包括泵室，一个或多个电极和微流体耦合到所述塔的入口的出口; 和（B）包括前表面和后表面的储存器芯片，其中所述储存器芯片在所述后表面中具有一个或多个空腔，当所述储存器芯片靠近所述主芯片的前表面设置时，所述泵室的体积将一个 的泵系统。 可以使用微加工来制备芯片，其可以用盖组装并插入用于电子控制和质谱分析的测试夹具中。 展示了与现有商业系统竞争的肽分离。

公开(公告)号：WO2004085063A2

公开(公告)日：2004-10-07

申请号：PCT/US2004/008331

申请日：2004-03-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  XIE, Jun ,  SHIH, Jason ,  TAI, Yu-Chong

Inventor： XIE, Jun ,  SHIH, Jason ,  TAI, Yu-Chong

IPC: B01L

CPC classification number: G01N27/226 ,  B81B7/02 ,  B81B2201/0292 ,  B81B2201/058 ,  G01L9/0042 ,  G01L9/0072

Abstract: A microfluidic device and method for capacitive sensing. The device includes a fluid channel including an inlet at a first end and an outlet at a second end, a cavity region coupled to the fluid channel, and a polymer based membrane coupled between the fluid channel and the cavity region. Additionally, the device includes a first capacitor electrode coupled to the membrane, a second capacitor electrode coupled to the cavity region and physically separated from the first capacitor electrode by at least the cavity region, and an electrical power source coupled between the first capacitor electrode and the second capacitor electrode and causing an electric field at least within the cavity region. The polymer based membrane includes a polymer.

Abstract translation: 用于电容感测的微流体装置和方法。 该装置包括流体通道，其包括在第一端处的入口和在第二端处的出口，耦合到流体通道的空腔区域以及耦合在流体通道和腔区域之间的基于聚合物的膜。 另外，该器件包括耦合到膜的第一电容器电极，耦合到空腔区域的第二电容器电极，并且至少通过腔区域与第一电容器电极物理分离;以及电源，耦合在第一电容器电极和 所述第二电容器电极至少在所述腔区域内引起电场。 基于聚合物的膜包括聚合物。

公开(公告)号：WO2004065830A1

公开(公告)日：2004-08-05

申请号：PCT/US2004/001048

申请日：2004-01-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  XIE, Jun ,  SHIH, Jason ,  TAI, Yu-Chong

Inventor： XIE, Jun ,  SHIH, Jason ,  TAI, Yu-Chong

IPC: F16K31/02

CPC classification number: F16K99/0001 ,  F16K99/0015 ,  F16K99/0044 ,  F16K99/0051 ,  F16K99/0061 ,  F16K2099/0074 ,  F16K2099/008 ,  G01F1/6845 ,  G01F15/005 ,  G05D7/0694 ,  Y10T29/49412 ,  Y10T137/0391

Abstract: A surface-micromachined mass flow controller (MFC) comprises an electrostatically actuated microvalve integrated with a thermal flow sensor. The microvalve comprises a normally-open diaphragm defining an aperture allowing fluid communication between first and second flow channels. The diaphragm includes a second electrode actuable toward a valve seat including a first electrode. Fabricated utilizing a multilayer Parylene process, the active microvalve and the flow sensor are integrated onto a single chip to perform closed-loop flow control. For flow control, both Pulse Width Modulation (PWM) and actuation voltage adjustment are demonstrated.

Abstract translation: 表面微加工质量流量控制器（MFC）包括与热流量传感器集成的静电致动微型阀。 微型阀包括限定允许第一和第二流动通道之间流体连通的孔的常开隔膜。 隔膜包括能够朝向包括第一电极的阀座致动的第二电极。 采用多层Parylene工艺制造，将活性微型阀和流量传感器集成到单个芯片上，以执行闭环流量控制。 对于流量控制，展示了脉宽调制（PWM）和启动电压调节。

公开(公告)号：EP1572576A2

公开(公告)日：2005-09-14

申请号：EP03739310.5

申请日：2003-06-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： TAI, Yu-Chong ,  XIE, Jun ,  HE, Qing ,  LEE, Terry, Dwight

IPC: B81C1/00

CPC classification number: G01N30/6095 ,  B01L3/0268 ,  B01L3/50273 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2400/046 ,  F04B17/00 ,  F04B19/006 ,  G01N30/32 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/326 ,  H01J49/04

Abstract: Electrochemical actuation is adopted in an integrated microfluidic chip to transfer fluid for sample preparation, separation and detection. The electrochemical actuation is capable of producing high pressure for on-chip fluidic handling. Technologies and methods are also developed to use only electrical source to control on-chip fluid handling without any external fluidic support. Applications for the devices and methods include micro scale HPLC, ESI-MS, etc.

公开(公告)号：EP1603672A2

公开(公告)日：2005-12-14

申请号：EP04757825.7

申请日：2004-03-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： XIE, Jun ,  SHIH, Jason ,  TAI, Yu-Chong

IPC: B01L1/00

CPC classification number: G01N27/226 ,  B81B7/02 ,  B81B2201/0292 ,  B81B2201/058 ,  G01L9/0042 ,  G01L9/0072

Abstract: A microfluidic device and method for capacitive sensing. The device includes a fluid channel including an inlet at a first end and an outlet at a second end, a cavity region coupled to the fluid channel, and a polymer based membrane coupled between the fluid channel and the cavity region. Additionally, the device includes a first capacitor electrode coupled to the membrane, a second capacitor electrode coupled to the cavity region and physically separated from the first capacitor electrode by at least the cavity region, and an electrical power source coupled between the first capacitor electrode and the second capacitor electrode and causing an electric field at least within the cavity region. The polymer based membrane includes a polymer.