公开(公告)号：WO2023027978A1

公开(公告)日：2023-03-02

申请号：PCT/US2022/041012

申请日：2022-08-22

Applicant: EMD MILLIPORE CORPORATION

Inventor： WU, Kevin ,  WOLFE, Kelly ,  DEDEO, Anja ,  AMARA, Ryan ,  LIBBY, Gerard ,  SYDLOWSKI, Paul ,  KAVANAUGH, Katelyn ,  BAILEY, Trisha ,  ALLEN, Nathan T.

IPC: G01N27/447 ,  G01N27/26

Abstract: An acrylamide gel formulation for use in electrophoresis, and a system and method for electrophoretic gel casting. In some embodiments, the system comprises a UV-Vis curing light source, and an acrylamide formulation that allows a resolving gel and a stacking gel to be photopolymerized in a single step. In some embodiments, the resolving solution formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP) as a water-soluble photoinitiator, which allows the solution to be photopolymerized using UV radiation within a certain wavelength. In some embodiments, a dilution buffer formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP). The dilution buffer may be added to the resolving solution in a selected amount in order to modify the concentration of the acrylamide to a desired concentration.

公开(公告)号：EP4392769A1

公开(公告)日：2024-07-03

申请号：EP22861932.6

申请日：2022-08-22

Applicant: EMD Millipore Corporation

Inventor： WU, Kevin ,  WOLFE, Kelly ,  DEDEO, Anja ,  AMARA, Ryan ,  LIBBY, Gerard ,  SYDLOWSKI, Paul ,  KAVANAUGH, Katelyn ,  BAILEY, Trisha ,  ALLEN, Nathan T.

IPC: G01N27/447 ,  G01N27/26

CPC classification number: G01N27/44747