公开(公告)号：WO2020146565A1

公开(公告)日：2020-07-16

申请号：PCT/US2020/012829

申请日：2020-01-09

Applicant: EMD MILLIPORE CORPORATION

Inventor： GREENIZEN, Kurt ,  SCOTT, Christopher ,  SYDLOWSKI, Paul ,  WU, Kevin ,  AMARA, Ryan ,  MANNING, Ryan ,  GUTIERREZ, Sara ,  DOYLE, John

IPC: G01N27/447

Abstract: The embodiments disclosed herein are directed to a device and assembly useful for hand casting an electrophoretic separation medium. The device includes a cassette frame 10 comprising first and second arms or members 12,22 pivotally connected to one another. One or more hinges 35A,35B may be provided to allow the first member to swing or pivot relative to the second member 12,22 (or vice versa) between a cassette frame open position and a cassette frame closed position. The first and second members 12,22 each include a substrate 20,30 to maintain a gel interface, preferably a glass plate substrate. The assembly includes a base and clamping arms that cooperate to hold one or more cassette frames in compressed relation.

公开(公告)号：WO2023027978A1

公开(公告)日：2023-03-02

申请号：PCT/US2022/041012

申请日：2022-08-22

Applicant: EMD MILLIPORE CORPORATION

Inventor： WU, Kevin ,  WOLFE, Kelly ,  DEDEO, Anja ,  AMARA, Ryan ,  LIBBY, Gerard ,  SYDLOWSKI, Paul ,  KAVANAUGH, Katelyn ,  BAILEY, Trisha ,  ALLEN, Nathan T.

IPC: G01N27/447 ,  G01N27/26

Abstract: An acrylamide gel formulation for use in electrophoresis, and a system and method for electrophoretic gel casting. In some embodiments, the system comprises a UV-Vis curing light source, and an acrylamide formulation that allows a resolving gel and a stacking gel to be photopolymerized in a single step. In some embodiments, the resolving solution formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP) as a water-soluble photoinitiator, which allows the solution to be photopolymerized using UV radiation within a certain wavelength. In some embodiments, a dilution buffer formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP). The dilution buffer may be added to the resolving solution in a selected amount in order to modify the concentration of the acrylamide to a desired concentration.

公开(公告)号：EP4392769A1

公开(公告)日：2024-07-03

申请号：EP22861932.6

申请日：2022-08-22

Applicant: EMD Millipore Corporation

Inventor： WU, Kevin ,  WOLFE, Kelly ,  DEDEO, Anja ,  AMARA, Ryan ,  LIBBY, Gerard ,  SYDLOWSKI, Paul ,  KAVANAUGH, Katelyn ,  BAILEY, Trisha ,  ALLEN, Nathan T.

IPC: G01N27/447 ,  G01N27/26

CPC classification number: G01N27/44747