公开(公告)号：CA2918036C

公开(公告)日：2018-04-24

申请号：CA2918036

申请日：2014-07-11

Applicant: FLODESIGN SONICS INC

Inventor： KENNEDY THOMAS J ,  LIPKENS BART ,  MASI LOUIS ,  KOWALSKI STANLEY ,  LEIDEL CHRIS

IPC: C12N13/00 ,  C12M1/42 ,  C12M3/00 ,  C12N5/071 ,  C12P21/00

Abstract: A series of multi-dimensional acoustic standing waves is set up inside a growth volume of a bioreactor. The acoustic standing waves are used to hold a cell culture in place as a nutrient fluid stream flows through the cell culture. Biomolecules produced by the cell culture are collected by the nutrient fluid stream and separated downstream of the cell culture.

公开(公告)号：CA2918036A1

公开(公告)日：2015-01-15

申请号：CA2918036

申请日：2014-07-11

Applicant: FLODESIGN SONICS INC

Inventor： KENNEDY THOMAS J III ,  LIPKENS BART ,  MASI LOUIS ,  KOWALSKI STANLEY III ,  LEIDEL CHRIS

IPC: C12N13/00 ,  C12M1/00

Abstract: A series of multi-dimensional acoustic standing waves is set up inside a growth volume of a bioreactor. The acoustic standing waves are used to hold a cell culture in place as a nutrient fluid stream flows through the cell culture. Biomolecules produced by the cell culture are collected by the nutrient fluid stream and separated downstream of the cell culture.

公开(公告)号：CA3034801A1

公开(公告)日：2018-03-01

申请号：CA3034801

申请日：2016-08-23

Applicant: FLODESIGN SONICS INC

Inventor： KENNEDY THOMAS J ,  LIPKENS BART ,  MASI LOUIS ,  KOWALSKI STANLEY ,  LEIDEL CHRIS

IPC: C12M1/00 ,  B01D21/28

Abstract: A series of multi-dimensional acoustic standing waves is set up inside a growth volume of a bioreactor. The acoustic standing waves are used to hold a cell culture in place as a nutrient fluid stream flows through the cell culture. The nutrient fluid stream dislodges some cells from the cell culture, which can then be recovered for cell therapy applications. The cell culture continues to expand and reproduce, permitting continuous recovery of cells from the bioreactor.

公开(公告)号：CA3023090A1

公开(公告)日：2017-11-09

申请号：CA3023090

申请日：2017-05-03

Applicant: FLODESIGN SONICS INC

Inventor： LIPKENS BART ,  PRESZ WALTER M ,  DUTRA BRIAN ,  GILMANSHIN RUDOLF ,  DIONNE JASON ,  LEIDEL CHRIS ,  MASI LOUIS ,  GHOSHAL GOUTAM

IPC: A61J3/00 ,  A61K35/12 ,  A61K35/14 ,  C12M1/00 ,  C12N13/00

Abstract: Multi-stage acoustophoretic devices for continuously separating a second fluid or a particulate from a host fluid are disclosed. Methods of operating the multi-stage acoustophoretic devices are also disclosed. The systems include multiple acoustophoretic devices fluidly connected to one another in series, each acoustophoretic device comprising a flow chamber, an ultrasonic transducer capable of creating a multi-dimensional acoustic standing wave, and a reflector. The systems can further include pumps and flowmeters.

公开(公告)号：ES2902047T3

公开(公告)日：2022-03-24

申请号：ES16758359

申请日：2016-08-23

Applicant: FLODESIGN SONICS INC

Inventor： KENNEDY THOMAS ,  LIPKENS BART ,  MASI LOUIS ,  KOWALSKI STANLEY ,  LEIDEL CHRIS

IPC: C12M1/00 ,  B01D21/28

Abstract: Procedimiento para la recolección continua de células a partir de un cultivo celular, que comprende: suspender el cultivo celular en un volumen de crecimiento de un biorreactor, incluyendo el biorreactor por lo menos un transductor ultrasónico y un reflector situado enfrente del por lo menos un transductor ultrasónico, activándose cada transductor ultrasónico para producir una onda estacionaria acústica multidimensional que mantiene el cultivo celular en el volumen de crecimiento, y hacer fluir una corriente de fluido nutriente a través del cultivo celular para hacer que se desprendan células del cultivo celular, y separar las células desprendidas de la corriente de fluido nutriente.

公开(公告)号：EP3019606A4

公开(公告)日：2017-04-05

申请号：EP14823682

申请日：2014-07-11

Applicant: FLODESIGN SONICS INC

Inventor： KENNEDY III THOMAS J ,  LIPKENS BART ,  MASI LOUIS ,  KOWALSKI III STANLEY ,  LEIDEL CHRIS

IPC: C12N13/00 ,  B06B1/02 ,  B06B1/06 ,  C07K1/14 ,  C12M1/00 ,  C12P21/02

CPC classification number: C12N13/00 ,  B06B1/0238 ,  B06B1/0648 ,  B06B2201/76 ,  C07K1/145 ,  C12M47/02 ,  C12P21/02

Abstract: A series of multi-dimensional acoustic standing waves is set up inside a growth volume of a bioreactor. The acoustic standing waves are used to hold a cell culture in place as a nutrient fluid stream flows through the cell culture. Biomolecules produced by the cell culture are collected by the nutrient fluid stream and separated downstream of the cell culture.

Abstract translation: 在生物反应器的生长体积内建立一系列多维声驻波。 声波驻波被用于将细胞培养物保持在适当位置，因为营养液流流过细胞培养物。 通过营养液流收集由细胞培养产生的生物分子，并在细胞培养物的下游分离。