MICROFLUIDIC CHECK VALVES
    1.
    发明申请
    MICROFLUIDIC CHECK VALVES 审中-公开
    微流感检查阀

    公开(公告)号:WO2008043046A3

    公开(公告)日:2008-08-07

    申请号:PCT/US2007080489

    申请日:2007-10-04

    Abstract: A microfluidic check valve and a method of using the check valve In microfluidic devices includes a check valve comprising two stacked chambers that are separated by a pore-containing membrane. The membrane is composed of an elastomeric material and can be configured in normally open or normally closed state. The normally open check valve can be implemented so that the degree of back pressure necessary to close the valve can be set.

    Abstract translation: 微流体止回阀和使用止回阀的方法在微流体装置中包括止回阀,其包括由含孔膜隔开的两个堆叠室。 膜由弹性体材料构成,可以配置成常开或常闭状态。 可以实现常开止回阀,使得可以设定关闭阀所需的背压程度。

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    4.
    发明公开
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 有权
    方法,系统和设备采集和处理几个单独的细胞的微流控芯片

    公开(公告)号:EP2820394A4

    公开(公告)日:2015-07-08

    申请号:EP13754093

    申请日:2013-02-28

    Applicant: FLUIDIGM CORP

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    Abstract translation: 方法,系统和装置被描述为多个单细胞捕获和处理利用微流体。 提供了用于捕获,分区和/或个体细胞从细胞中产生地连同遗传信息和/或与每个单独的电池反应的更大的人口操纵的工具和技术。 不同的捕获配置可以被用于捕获个人的细胞,然后在处理的多室反应配置每个单独的小区。 一些实施例可以提供特定目标扩增,全基因组扩增,全转录扩增,实时PCR制备,拷贝数变异,预扩增,测序mRNA和/或多个单独的电池单元型thathave是来自于较大的人口划分 细胞。 一些实施例可以提供用于其它应用。 一些实施例可以被配置为单个细胞或相关联的反应产物进行成像作为处理的一部分。 反应产物可以收获和/或在某些情况下,进一步的分析。

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS

    公开(公告)号:CA2878787A1

    公开(公告)日:2013-09-06

    申请号:CA2878787

    申请日:2013-02-28

    Applicant: FLUIDIGM CORP

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS

    公开(公告)号:SG11201405235VA

    公开(公告)日:2014-11-27

    申请号:SG11201405235V

    申请日:2013-02-28

    Applicant: FLUIDIGM CORP

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS

    公开(公告)号:SG10201608159XA

    公开(公告)日:2016-11-29

    申请号:SG10201608159X

    申请日:2013-02-28

    Applicant: FLUIDIGM CORP

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    MICROFLUIDIC DEVICE REGENERATION
    10.
    发明专利

    公开(公告)号:CA2782181A1

    公开(公告)日:2011-06-03

    申请号:CA2782181

    申请日:2010-11-30

    Applicant: FLUIDIGM CORP

    Abstract: A method for rendering a microfluidic device suitable for reuse for nucleic acid analysis is provided. The method may include flowing a nucleic acid inactivating solution into a microfluidic channel of the device by pumping; and then flowing a wash solution into the channel by pumping, thereby displacing the nucleic acid inactivating solution from the channel, whereby any residual nucleic acid from a prior use of the device is inactivated.

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