公开(公告)号：AU674694B2

公开(公告)日：1997-01-09

申请号：AU4761093

申请日：1993-08-10

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA YOSHIKAZU ,  KISHI YUICHIRO

IPC: C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58 ,  C12Q1/28 ,  C12Q1/42

Abstract: A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine containing, bound thereto, a label capable of generating a detectable signal or a precursor thereof to thereby form a complex between a nucleic acid, if any, and the polyamine, removing the polyamine which has not formed any complex before or after the conversion of the precursor, if used, into the label, and detecting the label.

公开(公告)号：EP0725149A4

公开(公告)日：1999-08-18

申请号：EP95907490

申请日：1993-08-10

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA YOSHIKAZU ,  KISHI YUICHIRO

IPC: C12Q1/68 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58 ,  C12Q1/28 ,  C12Q1/42

CPC classification number: C12Q1/6816 ,  C12Q1/6813

Abstract: A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine containing, bound thereto, a label capable of generating a detectable signal or a precursor thereof to thereby form a complex between a nucleic acid, if any, and the polyamine, removing the polyamine which has not formed any complex before or after the conversion of the precursor, if used, into the label, and detecting the label.

Abstract translation: 一种检测核酸的方法，其包括将怀疑携带或含有核酸的固体载体与含有与其结合的多胺接触能够产生可检测信号的标记或其前体的标记，从而在 核酸（如果有的话）和多胺，除去在前体转化之前或之后没有形成任何复合物的多胺（如果使用的话）到标签中，并检测标签。

公开(公告)号：CA2169166A1

公开(公告)日：1995-02-16

申请号：CA2169166

申请日：1993-08-10

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA YOSHIKAZU ,  KISHI YUICHIRO

IPC: C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58

Abstract: A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine containing, bound thereto, a label capable of generating a detectable signal or a precursor thereof to thereby form a complex between a nucleic acid, if any, and the polyamine, removing the polyamine which has not formed any complex before or after the conversion of the precursor, if used, into the label, and detecting the label.

公开(公告)号：DE69331443D1

公开(公告)日：2002-02-14

申请号：DE69331443

申请日：1993-08-10

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA YOSHIKAZU ,  KISHI YUICHIRO

IPC: C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58 ,  C12Q1/28 ,  C12Q1/42

Abstract: A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine containing, bound thereto, a label capable of generating a detectable signal or a precursor thereof to thereby form a complex between a nucleic acid, if any, and the polyamine, removing the polyamine which has not formed any complex before or after the conversion of the precursor, if used, into the label, and detecting the label.

公开(公告)号：AT211770T

公开(公告)日：2002-01-15

申请号：AT95907490

申请日：1993-08-10

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA YOSHIKAZU ,  KISHI YUICHIRO

IPC: C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58 ,  C12Q1/28 ,  C12Q1/42

Abstract: A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine containing, bound thereto, a label capable of generating a detectable signal or a precursor thereof to thereby form a complex between a nucleic acid, if any, and the polyamine, removing the polyamine which has not formed any complex before or after the conversion of the precursor, if used, into the label, and detecting the label.

公开(公告)号：DE69331443T2

公开(公告)日：2002-09-05

申请号：DE69331443

申请日：1993-08-10

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA YOSHIKAZU ,  KISHI YUICHIRO

IPC: C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58 ,  C12Q1/28 ,  C12Q1/42

Abstract: A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine containing, bound thereto, a label capable of generating a detectable signal or a precursor thereof to thereby form a complex between a nucleic acid, if any, and the polyamine, removing the polyamine which has not formed any complex before or after the conversion of the precursor, if used, into the label, and detecting the label.

公开(公告)号：DK0725149T3

公开(公告)日：2002-02-18

申请号：DK95907490

申请日：1993-08-10

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA YOSHIKAZU ,  KISHI YUICHIRO

IPC: C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58 ,  C12Q1/28 ,  C12Q1/42

Abstract: A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine containing, bound thereto, a label capable of generating a detectable signal or a precursor thereof to thereby form a complex between a nucleic acid, if any, and the polyamine, removing the polyamine which has not formed any complex before or after the conversion of the precursor, if used, into the label, and detecting the label.

公开(公告)号：JPH0699A

公开(公告)日：1994-01-11

申请号：JP15934992

申请日：1992-06-18

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA GIICHI ,  KISHI YUICHIRO

IPC: C12Q1/68 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58

Abstract: PURPOSE:To provide a method for detecting nucleic acid which is easy, safe, reliable and simple. CONSTITUTION:A solid carrier suspected to attach or contain nucleic acid is brought into contact with a polyamine to which a label (or its precursor) capable of giving measurable signals is bound, forming a composite made up of the nucleic acid and the polyamine. In case the precursor is used, it is transformed into the label. Before and after the transformation, the residual polyamine not forming the above composite is eliminated and then the label is probed, thus detecting the nucleic acid.