公开(公告)号：AU2014386209A2

公开(公告)日：2016-09-29

申请号：AU2014386209

申请日：2014-12-19

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  HAJIPOURAN BENAM KAMBEZ ,  VILLENAVE REMI ,  HAMILTON GERALDINE A ,  HASSELL BRYAN ,  HINOJOSA CHRISTOPHER D

IPC: C12N5/02

Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

公开(公告)号：AU2014386209A1

公开(公告)日：2016-07-21

申请号：AU2014386209

申请日：2014-12-19

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  HAJIPOURAN BENAM KAMBEZ ,  VILLENAVE REMI ,  HAMILTON GERALDINE A ,  HASSELL BRYAN ,  HINOJOSA CHRISTOPHER D

IPC: C12N5/02

Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

公开(公告)号：AU2014386209B2

公开(公告)日：2021-03-11

申请号：AU2014386209

申请日：2014-12-19

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  HAJIPOURAN BENAM KAMBEZ ,  VILLENAVE REMI ,  HAMILTON GERALDINE A ,  HASSELL BRYAN ,  HINOJOSA CHRISTOPHER D ,  LUCCHESI CAROLINA

IPC: C12N5/02

Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

公开(公告)号：CA2934662A1

公开(公告)日：2015-09-17

申请号：CA2934662

申请日：2014-12-19

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  HAJIPOURAN BENAM KAMBEZ ,  VILLENAVE REMI ,  HAMILTON GERALDINE A ,  HASSELL BRYAN ,  HINOJOSA CHRISTOPHER D

IPC: C12M3/02

Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

公开(公告)号：WO2015138034A3

公开(公告)日：2015-12-10

申请号：PCT/US2014071611

申请日：2014-12-19

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  HAJIPOURAN BENAM KAMBEZ ,  VILLENAVE REMI ,  HAMILTON GERALDINE A ,  HASSELL BRYAN ,  HINOJOSA CHRISTOPHER D

IPC: C12N5/02

CPC classification number: G01N33/5044 ,  B01L3/5027 ,  C12M21/08 ,  C12M23/16 ,  C12M25/02 ,  C12M35/04 ,  C12M41/46 ,  G01N2800/122

Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

Abstract translation: 本文涉及用于生产和使用具有由一个或多个隔膜分隔的中心通道的主体的系统和方法。 膜被配置为将中心通道分成至少一个中间通道和至少一个微通道。 中间通道的高度明显大于微通道的高度。 气体流体可以通过中间通道施加，同时液体流体流过微通道。 本文描述的系统和方法可用于各种应用，包括例如原代细胞如人肺细胞的生长和分化以及需要低剪切和/或分层结构的任何其它细胞，或微环境的模拟 活组织和/或器官（用于模拟生理学或疾病状态，和/或鉴定治疗剂和/或疫苗）。 系统和方法还可以允许与一种或多种不同细胞类型的共培养。

公开(公告)号：EP3014329A4

公开(公告)日：2016-07-06

申请号：EP14818128

申请日：2014-06-26

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  LEVNER DANIEL ,  THOMPSON GUY ,  HINOJOSA CHRISTOPHER D ,  FERNANDEZ-ALCON JOSE

IPC: G02B21/24 ,  G02B21/26

CPC classification number: G02B21/24 ,  G02B7/04 ,  G02B21/02 ,  G02B21/06 ,  G02B21/14 ,  G02B21/16 ,  G02B21/26 ,  G02B21/34 ,  G02B21/36

Abstract: A microscopy system for monitoring of one or more specimens includes a plurality of microscope blades, each microscope blade having at least one objective, at least one illuminator, and at least one detector. The microscopy system also includes a plurality of carriages, each carriage being connected to one or more of the microscope blades, and one or more actuators configured to drive the plurality of carriages along one or more axes, at least some of the plurality of carriages having at least partially overlapping ranges of motion along at least one of the one or more axes. The microscopy system also includes a master controller configured to drive each of the carriages, using the actuator(s), along the one or more axes.

Abstract translation: 用于监测一个或多个试样的显微镜系统包括多个显微镜刀片，每个显微镜刀片具有至少一个物镜，至少一个照明器和至少一个检测器。 显微镜系统还包括多个滑架，每个滑架连接到一个或多个显微镜刀片，以及一个或多个致动器，其配置成沿着一个或多个轴线驱动多个滑架，多个滑架中的至少一些具有 沿着所述一个或多个轴中的至少一个的至少部分重叠的运动范围。 显微镜系统还包括主控制器，其构造成沿着一个或多个轴线使用致动器来驱动每个托架。

公开(公告)号：EP3083940A4

公开(公告)日：2017-08-23

申请号：EP14885765

申请日：2014-12-19

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  HAJIPOURAN BENAM KAMBEZ ,  VILLENAVE REMI ,  HAMILTON GERALDINE A ,  HASSELL BRYAN ,  HINOJOSA CHRISTOPHER D

IPC: C12M3/00 ,  B01L3/00 ,  C12M1/34 ,  G01N33/48

CPC classification number: G01N33/5044 ,  B01L3/5027 ,  C12M21/08 ,  C12M23/16 ,  C12M25/02 ,  C12M35/04 ,  C12M41/46 ,  G01N2800/122