公开(公告)号：WO2022231612A1

公开(公告)日：2022-11-03

申请号：PCT/US2021/030085

申请日：2021-04-30

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： CUMBIE, Michael ,  KEEFE, Brian J. ,  SAMLALI, Kenza ,  SHKOLNIKOV, Viktor

IPC: C12Q1/6806 ,  G01N33/569 ,  G01N21/68 ,  B01F3/08 ,  B81B1/00

Abstract: A PCR system is described. The PCR system comprises a thermocycling chamber comprising an electrode assembly configured to generate an electric field and cause reversible electrowetting of a PCR mixture within the thermocycling chamber; and an optical sensor configured to obtain optical signals from the thermocycling chamber. Also described is a method of manufacturing a PCR system and a method of performing PCR.

公开(公告)号：WO2020122925A1

公开(公告)日：2020-06-18

申请号：PCT/US2018/065498

申请日：2018-12-13

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： HAMMERSTAD, Diane ,  GOVYADINOV, Alexander ,  KEEFE, Brian J. ,  TORNIAINEN, Erik D. ,  COCHELL, Tyler ,  KORNILOVICH, Pavel

IPC: C12M1/02 ,  C12M1/38 ,  C12Q1/6844 ,  G01N33/50 ,  B81B1/00

Abstract: A rapid thermal cycling device can include a static microfluidic reaction chamber that can be defined between a layered substrate and a cover that can have an average space therebetween from 4 µm to 150 µm. The layered substrate can include a heating element thermally coupled to the static microfluidic reaction chamber to heat a fluid when present therein. The layered substrate, the cover, or both can include a heat diffusing material thermally coupled to the static microfluidic reaction chamber to diffuse heat out from the fluid while remaining in the static microfluidic reaction chamber.

公开(公告)号：WO2023069108A1

公开(公告)日：2023-04-27

申请号：PCT/US2021/056133

申请日：2021-10-22

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： CUMBIE, Michael ,  SHKOLNIKOV, Viktor ,  KEEFE, Brian J.

IPC: C12Q1/686 ,  B81B7/02 ,  C12M1/38 ,  C12M1/02

Abstract: A PCR system is described. The PCR system comprises a microfluidic cartridge having a thermocycling chamber, wherein a floor of the thermocycling chamber is substantially planar and is provided with a heater; a cooling module configured to engage with and be in thermal contact with a surface of the microfluidic cartridge; and an optical sensor configured to obtain optical signals from the thermocycling chamber. A method of performing PCR is also described.

公开(公告)号：WO2022235272A1

公开(公告)日：2022-11-10

申请号：PCT/US2021/031344

申请日：2021-05-07

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： CUMBIE, Michael W. ,  SHKOLNIKOV, Viktor ,  KEEFE, Brian J.

IPC: C12Q1/6806 ,  G01N33/569 ,  B01F23/411 ,  B81B1/00

Abstract: An example overfill-tolerant microfluidic structure can include an inlet microfluidic channel. A sample chamber can be connected to the inlet microfluidic channel to receive liquid from the inlet microfluidic channel. A gas-permeable liquid barrier can be connected to the sample chamber and positioned to allow gas to flow out of the sample chamber. An overflow chamber can be connected to the inlet microfluidic channel. A capillary break can be positioned between the inlet microfluidic channel and the overflow chamber. The capillary break can include a narrowed opening with a smaller width than a width of the inlet microfluidic channel. In some examples, the gas-permeable liquid barrier can allow gas to flow out of the sample chamber at a pressure lower than the break pressure, and prevent liquid from flowing out of the sample chamber at the break pressure.

公开(公告)号：WO2022046079A1

公开(公告)日：2022-03-03

申请号：PCT/US2020/048486

申请日：2020-08-28

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： SASSER, Jacob Shane ,  GOVYADINOV, Alexander ,  KEEFE, Brian J. ,  CLAGUE, David S. ,  WEISENBURGER, Natalie Taylor ,  MEDJO, Nicolas Robert

IPC: C12Q1/68 ,  G01N33/48

Abstract: In one example in accordance with the present disclosure, a fluid manipulation system is described. The fluid manipulation system includes a microfluidic channel through which fluid is to flow. The fluid includes biomolecules to be separated. The fluid manipulation system also includes at least one array of biomolecule-capturing pillars disposed within the microfluidic channel to capture biomolecules from the fluid. Barriers rise from a surface of the microfluidic channel. The barriers span a width of the microfluidic channel orthogonal to a flow of the fluid to induce vortices in the fluid flow.

公开(公告)号：WO2022046078A1

公开(公告)日：2022-03-03

申请号：PCT/US2020/048470

申请日：2020-08-28

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： SASSER, Jacob Shane ,  GOVYADINOV, Alexander ,  KEEFE, Brian J. ,  CLAGUE, David S. ,  WEISENBURGER, Natalie Taylor ,  MEDJO, Nicolas Robert

IPC: C12Q1/68 ,  C12M1/36 ,  G01N27/26 ,  G01N33/48 ,  B81B1/00 ,  B81B7/04

Abstract: In one example in accordance with the present disclosure, a fluid manipulation system is described. The fluid manipulation system includes a microfluidic channel through which fluid is to flow. The fluid includes biomolecules to be separated. The fluid manipulation system also includes an array of biomolecule-capturing pillars disposed within the microfluidic channel to capture biomolecules from the fluid. The fluid manipulation system also includes a first electrode pair formed on opposing sides of the array of biomolecule-capturing pillars to generate an alternating electrical field across the microfluidic channel.