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公开(公告)号：JP2005058236A
公开(公告)日：2005-03-10
申请号：JP2004235542
申请日：2004-08-12
Inventor： ANKENBAUER WALTRAUD , MAIER THOMAS , DEUFEL ANNETTE , HEINDL DIETER , BETZL GISELA , SCHMUCK RAINER , SCHNEIDINGER BERND , STREY JESSICA
IPC: C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
CPC classification number: C12N9/1252
Abstract: PROBLEM TO BE SOLVED: To provide a peptide having an increased thermal stability and a thermally stable DNA polymerase activity and a method for forming a purified peptide. SOLUTION: Disclosed is a peptide having a specific amino acid sequence and a DNA polymerase activity. COPYRIGHT: (C)2005,JPO&NCIPI
Abstract translation: 待解决的问题：提供具有增加的热稳定性和热稳定的DNA聚合酶活性的肽以及形成纯化肽的方法。 解决方案：公开了具有特定氨基酸序列和DNA聚合酶活性的肽。 版权所有（C）2005，JPO＆NCIPI
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公开(公告)号：JP2003210199A
公开(公告)日：2003-07-29
申请号：JP2002337219
申请日：2002-11-20
Applicant: HOFFMANN LA ROCHE , IDAHO TECHNOLOGY INC
Inventor： TABITI KARIM DR , BETZL GISELA , SOONG RICHIE , RASMUSSEN RANDY , DESILVA DEEPIKA MARINE , WARD JOHN G , WILLWARD HALIEGH PAGE
Abstract: PROBLEM TO BE SOLVED: To provide a quantitative multiplex PCR concerning the field of real time PCR, especially the determination of a nucleic acid in a specific intracellular mRNA. SOLUTION: A method for the quantitative multiplex PCR with a high dynamic range involves using a thermostable DNA polymerase at a final concentration of at least 0.5 units/μl. A kit therefor is also provided. COPYRIGHT: (C)2003,JPO
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公开(公告)号：CA2407744A1
公开(公告)日：2003-05-20
申请号：CA2407744
申请日：2002-11-13
Applicant: HOFFMANN LA ROCHE , IDAHO TECHNOLOGY INC
Inventor： SOONG RICHIE , DESILVA DEEPIKA MARINE , TABITI KARIM , RASMUSSEN RANDY , WARD JOHN G , MILLWARD HALIEGH PAGE , BETZL GISELA
Abstract: The new invention provides a method and a kit for quantitative multiplex PCR with a high dynamic range characterized in that a thermostable DNA Polymerase with a fin al concentration of at least 0,5 units/.mu.l is use.
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公开(公告)号：CA2407744C
公开(公告)日：2012-01-03
申请号：CA2407744
申请日：2002-11-13
Applicant: HOFFMANN LA ROCHE , IDAHO TECHNOLOGY INC
Inventor： TABITI KARIM , BETZL GISELA , SOONG RICHIE , RASMUSSEN RANDY , DESILVA DEEPIKA MARINE , WARD JOHN G , MILLWARD HALIEGH PAGE
Abstract: The new invention provides a method and a kit for quantitative multiplex PCR with a high dynamic range characterized in that a thermostable DNA Polymerase with a final concentration of at least 0,5 units/µl is use.
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公开(公告)号：CA2474734C
公开(公告)日：2010-07-13
申请号：CA2474734
申请日：2004-08-11
Applicant: HOFFMANN LA ROCHE
Inventor： ANKENBAUER WALTRAUD , MEIER THOMAS , DEUFEL ANNETTE , HEINDL DIETER , BETZL GISELA , SCHMUCK RAINER , SCHNEIDINGER BERND , STREY JESSICA
IPC: C12N15/09 , C12N15/54 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq.DELTA.288) possesses an increased thermostability over TaqWT, Taq.DELTA.279, and Taq.DELTA.289. The present invention therefore provides Taq.DELTA.288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq.DELTA.288. The invention also encompasses kits containing Taq.DELTA.288 as well as the use of Taq.DELTA.288 and kits containing Taq.DELTA.288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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Patent Agency Ranking
公开(公告)号：AU2004203649A1
公开(公告)日：2005-03-03
申请号：AU2004203649
申请日：2004-08-06
Applicant: HOFFMANN LA ROCHE
Inventor： HEINDL DIETER , DEUFEL ANNETTE , MEIER THOMAS , ANKENBAUER WALTRAUD , STREY JESSICA , SCHNEIDINGER BERND , SCHMUCK RAINER , BETZL GISELA
IPC: C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq DELTA 288. The invention also encompasses kits containing Taq DELTA 288 as well as the use of Taq DELTA 288 and kits containing Taq DELTA 288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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公开(公告)号：ES2260378T3
公开(公告)日：2006-11-01
申请号：ES02025794
申请日：2002-11-16
Applicant: HOFFMANN LA ROCHE , IDAHO TECHNOLOGY INC
Inventor： TABITI KARIM DR , BETZL GISELA , SOONG RICHIE DR , RASMUSSEN RANDY , DESILVA DEEPIKA MARINE , WARD JOHN G
Abstract: Un método para una PCR multiplex cuantitativa con un intervalo dinámico de al menos 102, preferiblemente de 103 y más preferiblemente de 104, donde se utiliza una DNA polimerasa termoestable con una concentración final de al menos 0, 5 unidades/µl.
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公开(公告)号：AU2004203649B2
公开(公告)日：2006-01-12
申请号：AU2004203649
申请日：2004-08-06
Applicant: HOFFMANN LA ROCHE
Inventor： DEUFEL ANNETTE , STREY JESSICA , BETZL GISELA , ANKENBAUER WALTRAUD , SCHNEIDINGER BERND , MEIER THOMAS , HEINDL DIETER , SCHMUCK RAINER
IPC: C12N15/09 , C12N15/52 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq DELTA 288. The invention also encompasses kits containing Taq DELTA 288 as well as the use of Taq DELTA 288 and kits containing Taq DELTA 288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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公开(公告)号：BRPI0403221A
公开(公告)日：2005-07-12
申请号：BRPI0403221
申请日：2004-08-12
Applicant: HOFFMANN LA ROCHE
Inventor： ANKENBAUER WALTRAUD , MEIER THOMAS , DEUFEL ANNETTE , HEINDL DIETER , BETZL GISELA , SCHMUCK RAINER , SCHNEIDINGER BERND , STREY JESSICA
IPC: C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq DELTA 288. The invention also encompasses kits containing Taq DELTA 288 as well as the use of Taq DELTA 288 and kits containing Taq DELTA 288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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公开(公告)号：AT319859T
公开(公告)日：2006-03-15
申请号：AT02025794
申请日：2002-11-16
Applicant: HOFFMANN LA ROCHE , IDAHO TECHNOLOGY INC
Inventor： TABITI KARIM DR , BETZL GISELA , SOONG RICHIE DR , RASMUSSEN RANDY , DESILVA DEEPIKA MARINE , WARD JOHN G , WILLWARD HALIEGH PAGE
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