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公开(公告)号:JP2005058236A
公开(公告)日:2005-03-10
申请号:JP2004235542
申请日:2004-08-12
Inventor: ANKENBAUER WALTRAUD , MAIER THOMAS , DEUFEL ANNETTE , HEINDL DIETER , BETZL GISELA , SCHMUCK RAINER , SCHNEIDINGER BERND , STREY JESSICA
IPC: C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
CPC classification number: C12N9/1252
Abstract: PROBLEM TO BE SOLVED: To provide a peptide having an increased thermal stability and a thermally stable DNA polymerase activity and a method for forming a purified peptide. SOLUTION: Disclosed is a peptide having a specific amino acid sequence and a DNA polymerase activity. COPYRIGHT: (C)2005,JPO&NCIPI
Abstract translation: 待解决的问题:提供具有增加的热稳定性和热稳定的DNA聚合酶活性的肽以及形成纯化肽的方法。 解决方案:公开了具有特定氨基酸序列和DNA聚合酶活性的肽。 版权所有(C)2005,JPO&NCIPI
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公开(公告)号:CA2474734A1
公开(公告)日:2005-02-12
申请号:CA2474734
申请日:2004-08-11
Applicant: HOFFMANN LA ROCHE
Inventor: SCHNEIDINGER BERND , STREY JESSICA , ANKENBAUER WALTRAUD , DEUFEL ANNETTE , HEINDL DIETER , BETZL GISELA , SCHMUCK RAINER , MEIER THOMAS
IPC: C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq.DELTA.288) possesses an increased thermostability over TaqWT, Taq.DELTA.279, and Taq.DELTA.289. The present invention therefore provides Taq.DELTA.288, recombinant expression vectors encoding th e same or derivatives thereof, as well as purification protocols for Taq.DELTA.288. Th e invention also encompasses kits containing Taq.DELTA.288 as well as the use of Taq.DELTA.288 and kits containing Taq.DELTA.288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucle ic acid.
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公开(公告)号:AU2004203649B2
公开(公告)日:2006-01-12
申请号:AU2004203649
申请日:2004-08-06
Applicant: HOFFMANN LA ROCHE
Inventor: DEUFEL ANNETTE , STREY JESSICA , BETZL GISELA , ANKENBAUER WALTRAUD , SCHNEIDINGER BERND , MEIER THOMAS , HEINDL DIETER , SCHMUCK RAINER
IPC: C12N15/09 , C12N15/52 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq DELTA 288. The invention also encompasses kits containing Taq DELTA 288 as well as the use of Taq DELTA 288 and kits containing Taq DELTA 288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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公开(公告)号:BRPI0403221A
公开(公告)日:2005-07-12
申请号:BRPI0403221
申请日:2004-08-12
Applicant: HOFFMANN LA ROCHE
Inventor: ANKENBAUER WALTRAUD , MEIER THOMAS , DEUFEL ANNETTE , HEINDL DIETER , BETZL GISELA , SCHMUCK RAINER , SCHNEIDINGER BERND , STREY JESSICA
IPC: C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq DELTA 288. The invention also encompasses kits containing Taq DELTA 288 as well as the use of Taq DELTA 288 and kits containing Taq DELTA 288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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公开(公告)号:CA2474734C
公开(公告)日:2010-07-13
申请号:CA2474734
申请日:2004-08-11
Applicant: HOFFMANN LA ROCHE
Inventor: ANKENBAUER WALTRAUD , MEIER THOMAS , DEUFEL ANNETTE , HEINDL DIETER , BETZL GISELA , SCHMUCK RAINER , SCHNEIDINGER BERND , STREY JESSICA
IPC: C12N15/09 , C12N15/54 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq.DELTA.288) possesses an increased thermostability over TaqWT, Taq.DELTA.279, and Taq.DELTA.289. The present invention therefore provides Taq.DELTA.288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq.DELTA.288. The invention also encompasses kits containing Taq.DELTA.288 as well as the use of Taq.DELTA.288 and kits containing Taq.DELTA.288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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公开(公告)号:AU2004203649A1
公开(公告)日:2005-03-03
申请号:AU2004203649
申请日:2004-08-06
Applicant: HOFFMANN LA ROCHE
Inventor: HEINDL DIETER , DEUFEL ANNETTE , MEIER THOMAS , ANKENBAUER WALTRAUD , STREY JESSICA , SCHNEIDINGER BERND , SCHMUCK RAINER , BETZL GISELA
IPC: C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq DELTA 288. The invention also encompasses kits containing Taq DELTA 288 as well as the use of Taq DELTA 288 and kits containing Taq DELTA 288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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公开(公告)号:DK1507002T3
公开(公告)日:2007-04-10
申请号:DK04018913
申请日:2004-08-10
Applicant: HOFFMANN LA ROCHE
Inventor: ANKENBAUER WALTRAUD DR , MEIER THOMAS DR , DEUFEL ANNETTE DR , HEINDL DIETER DR , BETZL GISELA DR , SCHMUCK RAINER DR , SCHNEIDINGER BERND DR , STREY JESSICA
IPC: A61K38/45 , C12N9/12 , C07H21/04 , C12N15/09 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq DELTA 288. The invention also encompasses kits containing Taq DELTA 288 as well as the use of Taq DELTA 288 and kits containing Taq DELTA 288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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公开(公告)号:AT348881T
公开(公告)日:2007-01-15
申请号:AT04018913
申请日:2004-08-10
Applicant: HOFFMANN LA ROCHE
Inventor: ANKENBAUER WALTRAUD DR , MEIER THOMAS DR , DEUFEL ANNETTE DR , HEINDL DIETER DR , BETZL GISELA DR , SCHMUCK RAINER DR , SCHNEIDINGER BERND DR , STREY JESSICA
IPC: C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq DELTA 288) possesses an increased thermostability over TaqWT, Taq DELTA 279, and Taq DELTA 289. The present invention therefore provides Taq DELTA 288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq DELTA 288. The invention also encompasses kits containing Taq DELTA 288 as well as the use of Taq DELTA 288 and kits containing Taq DELTA 288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
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