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    Stable nad/nadh derivative
    1.
    发明专利
    Stable nad/nadh derivative 有权
    稳定的NAD / NADH衍生物

    公开(公告)号：JP2012224638A

    公开(公告)日：2012-11-15

    申请号：JP2012172878

    申请日：2012-08-03

    Applicant: F Hoffmann-La Roche Ag ,  エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト

    Inventor： HOENES JOACHIM ,  HEINDL DIETER ,  HORN CARINA ,  GAESSLER-DIETSCHE CLAUDIA

    IPC: C07H19/207 ,  G01N33/52 ,  G01N33/66

    CPC classification number: C12Q1/32

    Abstract: PROBLEM TO BE SOLVED: To provide a stable bioanalytical measuring system, in particular for determining glucose, which avoids the sensitivity to hydrolysis of NAD/NADP and at the same time acts as a coenzyme in an enzyme reaction.SOLUTION: Nicotinamide adenine dinucleotide (NAD/NADH) derivatives and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives represented by formula (I), enzyme complexes of these derivatives, their use in biochemical detection methods, and reagent kits thereof are provided.

    Abstract translation: 要解决的问题：提供稳定的生物分析测量系统，特别是用于测定葡萄糖，其避免对NAD / NADP水解的敏感性，并且同时作为酶反应中的辅酶。 解决方案：由式（I）表示的烟酰胺腺嘌呤二核苷酸（NAD / NADH）衍生物和烟酰胺腺嘌呤二核苷酸磷酸（NADP / NADPH）衍生物，这些衍生物的酶复合物，它们在生物化学检测方法中的用途及其试剂盒是 提供。 版权所有（C）2013，JPO＆INPIT

    Fluorescence spectroscopy in absorption medium
    2.
    发明专利
    Fluorescence spectroscopy in absorption medium 审中-公开
    吸收介质中的荧光光谱

    公开(公告)号：JP2007121301A

    公开(公告)日：2007-05-17

    申请号：JP2006290284

    申请日：2006-10-25

    Applicant: F Hoffmann-La Roche Ag ,  エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト

    Inventor： PETRICH WOLFGANG ,  GAESSLER-DIETSCHE CLAUDIA ,  HORN CARINA ,  HEBESTREIT KAI ,  ASFOUR JEAN-MICHEL ,  BARDELANG KLEMENS ,  KOCHERSCHEIDT GERRIT

    IPC: G01N21/64

    CPC classification number: C12Q1/008 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/54 ,  G01N33/52 ,  G01N33/66 ,  Y10T436/144444

    Abstract: PROBLEM TO BE SOLVED: To remarkably reduce a dependency with respect to a wavelength of fluorescent excitation light, and to change the fluorescent excitation maximum of a fluorescence emission group. SOLUTION: A detecting medium containing the fluorescence emission group or a precursor of the fluorescence emission group is mixed with an absorber having an absorption spectrum overlapped with a fluorescent excitation range of the fluorescence emission group. A system generated in the detecting medium and comprising the fluorescence emission group and the absorber has a changed effective fluorescent excitation range accompanied with the changed fluorescent excitation maximum. Irradiation by the fluorescent excitation light is executed within the range of the changed excitation maximum. A measured signal obtained from fluorescence emission measurement indicates only the low dependency with respect to the wavelength of the excitation light. Further, an inexpensive light source such as UV LEDs may be usable because of the changed wave length of the excitation light. COPYRIGHT: (C)2007,JPO&INPIT

    Abstract translation: 要解决的问题：显着降低相对于荧光激发光的波长的依赖性，并且改变荧光发射基团的荧光激发最大值。 解决方案：将含有荧光发射基团或荧光发射基团的前体的检测介质与具有与荧光发射基团的荧光激发范围重叠的吸收光谱的吸收剂混合。 在检测介质中产生并且包括荧光发射基团和吸收体的系统具有伴随改变的荧光激发最大值的改变的有效荧光激发范围。 通过荧光激发光的照射在变化的激发最大值的范围内进行。 从荧光发射测量获得的测量信号仅表示相对于激发光的波长的低依赖性。 此外，由于激发光的波长变化，可以使用诸如UV LED的便宜的光源。 版权所有（C）2007，JPO＆INPIT

    Derivados estables de NAD/NADH
    6.
    发明专利
    Derivados estables de NAD/NADH 未知

    公开(公告)号：ES2461266T3

    公开(公告)日：2014-05-19

    申请号：ES06776486

    申请日：2006-07-28

    Applicant: HOFFMANN LA ROCHE

    Inventor： HOENES JOACHIM ,  HEINDL DIETER ,  HORN CARINA ,  GAESSLER-DIETSCHE CLAUDIA

    IPC: C07H19/207 ,  C07H19/213 ,  C07H21/00 ,  C07H21/02 ,  C07H23/00 ,  C12Q1/32

    Abstract: Elemento de ensayo para la determinación de glucosa, que comprende (i) una glucosa-deshidrogenasa (EC 1.1.1.47) y (ii) como coenzima un compuesto de la siguiente fórmula general (I):**Fórmula** en el cual A >= adenina o un análogo de ella, T >= O, S respectivamente independientes, U >= OH, SH, BH3 -, BCNH2 - respectivamente independientes, V >= OH respectivamente independiente o un grupo fosfato, de modo que en caso de que un radical V sea un grupo OH y el segundo radical V un grupo fostato, el grupo OH y el grupo fostato pueden formar un anillo con los átomos de carbono a los que están unidos; W >= COOR, CON(R)2, COR, CSN(R)2, donde R >= H o alquilo C1-C2 respectivamente independientes, X1, X2 >= O, CH2, CHCH3, C(CH3)2, NH, NCH3 respectivamente independientes, Y >= NH, S, O, CH2, Z >= un radical, incluyendo un grupo cíclico de 5 átomos de C que puede llevar un heteroátomo escogido entre O, S y N, así como uno o más sustituyentes, y un radical CR42 unido al grupo cíclico y a X2, donde R4 >= H, F, Cl, CH3 respectivamente independientes, con la condición de que Z y el radical piridino no estén unidos por un enlace glicosídico, o una sal o, dado el caso, una forma reducida del mismo.

    STABILIZATION OF DEHYDROGENASES USING STABLE CHEMICALLY MODIFIED COENZYMES
    10.
    发明专利
    STABILIZATION OF DEHYDROGENASES USING STABLE CHEMICALLY MODIFIED COENZYMES 未知

    公开(公告)号：NZ587397A

    公开(公告)日：2012-04-27

    申请号：NZ58739709

    申请日：2009-02-19

    Applicant: HOFFMANN LA ROCHE

    Inventor： HEINDL DIETER ,  HORN CARINA ,  GAESSLER-DIETSCHE CLAUDIA ,  HOENES JOACHIM

    IPC: C12N9/96 ,  C12N9/06 ,  C12Q1/32

    Abstract: Disclosed is a method for stabilizing a coenzyme dependent enzyme, characterized in that the enzyme is stored in the presence of the coenzyme, said coenzyme being a stable coenzyme chemically modified by comparison to, and having a higher stability than, the native coenzyme. Also disclosed is a coenzyme dependent enzyme which is stabilized with the coenzyme, said coenzyme being a stable coenzyme chemically modified by comparison to, and having a higher stability than, the native coenzyme, characterized in that it shows on storage for at least 2 weeks at a temperature of at least 20 degrees C, where appropriate with high humidity and without a drying reagent, a decline in the enzymatic activity of less than 50% compared with the initial level, wherein the enzyme is a mutated glucose dehydrogenase and the mutated glucose dehydrogenase has an increased thermal or hydrolytic stability compared with the corresponding wild-type glucose dehydrogenase.

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