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公开(公告)号：JP2011217746A
公开(公告)日：2011-11-04
申请号：JP2011085864
申请日：2011-04-08
Inventor： FISCHER ULRIKE , GREIF MICHAEL , SOBEK HARALD , THALHOFER JOHANN-PETER
CPC classification number: C12Q1/686 , C12N9/1252 , C12Q2527/137 , C12Q2521/101
Abstract: PROBLEM TO BE SOLVED: To provide an improved polymerase composition with optimized performance in a polymerase chain reaction (PCR).SOLUTION: The thermostable DNA polymerase composition completely free from surfactant contains 10-50 mM Tris/HCl, 0.05-0.2 mM EDTA, 0.5-2 mM DTT, 50-200 mM potassium chloride, and 20-80% glycerol.
Abstract translation: 待解决的问题：提供在聚合酶链反应（PCR）中具有优化性能的改进的聚合酶组合物。 解决方案：完全不含表面活性剂的热稳定DNA聚合酶组合物含有10-50mM Tris / HCl，0.05-0.2mM EDTA，0.5-2mM DTT，50-200mM氯化钾和20-80％甘油。 版权所有（C）2012，JPO＆INPIT
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公开(公告)号：JP2010142229A
公开(公告)日：2010-07-01
申请号：JP2009286615
申请日：2009-12-17
Inventor： HASLINGER TOBIAS , METZLER THOMAS DR , PECENY ANNETTE , SOBEK HARALD
IPC: C12N15/09 , C12N9/12 , C12N15/115 , C12Q1/68
CPC classification number: C12Q1/686 , C12N9/1252 , C12Q1/6876
Abstract: PROBLEM TO BE SOLVED: To provide a method for obtaining a dried composition of reactive compounds, maintaining the living body activity of the compounds on re-dissolving after a certain storage period. SOLUTION: This method for producing the dried composition capable of storing the reactive compounds comprises (a) a process of preparing a liquid composition which is a liquid mixture of the reactive compounds, containing a primer, nucleotide, Taq DNA polymerase and first stabilizing molecule, and (b) a process of drying the liquid mixture by reducing the surrounding pressure of the liquid mixture, wherein, the dried composition of the reactive compounds is dissolvable with an aqueous solution, and the liquid mixture of the reactive compounds in the process (a) contains further an aptamer as a second stabilizing molecule. COPYRIGHT: (C)2010,JPO&INPIT
Abstract translation: 要解决的问题：提供一种获得干燥的反应性化合物组合物的方法，在一定的储存期后维持化合物在再溶解时的活体活性。 解决方案：用于制备能够储存反应性化合物的干燥组合物的方法包括（a）制备液体组合物的方法，该液体组合物是含有引物，核苷酸，Taq DNA聚合酶和第一反应物的反应性化合物的液体混合物 稳定分子，和（b）通过降低液体混合物的周围压力来干燥液体混合物的方法，其中反应性化合物的干燥组合物可用水溶液溶解，并且反应性化合物的液体混合物在 方法（a）还包含作为第二稳定分子的适体。 版权所有（C）2010，JPO＆INPIT
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3.发明专利Stabilized aqueous alpha-galactosidase composition and related method to the same 有权稳定的水性α-乳糖苷酶组合物及其相关方法
公开(公告)号：JP2010252793A
公开(公告)日：2010-11-11
申请号：JP2010099666
申请日：2010-04-23
Inventor： HOELKE WERNER , MUELLER RAINER , SCHMIDT MANFRED , SOBEK HARALD , THALHOFER JOHANN-PETER
CPC classification number: C07K14/435
Abstract: PROBLEM TO BE SOLVED: To provide an aqueous composition containing a protein having enzymatic activity of an alpha-galactosidase.
SOLUTION: This method for stabilizing the aqueous composition containing the protein having the enzymatic activity of the alpha-galactosidase is provided. The method for preparing the purified aqueous composition containing the protein having the enzymatic activity of the alpha-galactosidase is also provided.
COPYRIGHT: (C)2011,JPO&INPITAbstract translation: 待解决的问题：提供含有具有α-半乳糖苷酶的酶活性的蛋白质的含水组合物。 解决方案：提供了用于稳定含有具有α-半乳糖苷酶的酶活性的蛋白质的含水组合物的方法。 还提供了制备含有具有α-半乳糖苷酶的酶活性的蛋白质的纯化含水组合物的方法。 版权所有（C）2011，JPO＆INPIT
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公开(公告)号：JP2011224002A
公开(公告)日：2011-11-10
申请号：JP2011090825
申请日：2011-04-15
Inventor： GREIF MICHAEL , MUELLER RAINER , RUDOLPH CHRISTIAN , RUF ARMIN , SCHMIDT MANFRED , SOBEK HARALD , THALHOFER JOHANN-PETER
CPC classification number: C12N9/1247 , C12P19/34 , C12Y207/07006
Abstract: PROBLEM TO BE SOLVED: To provide an improved variant of T7 RNA polymerase by introducing new mutation leading to improved thermostability of the enzyme.SOLUTION: There is provided a variant of T7 RNA polymerase leading to improved thermostability by introducing a new mutation at positions Val426, Ser633, Val650, Thr654, Ala702, Val795 and combinations thereof.
Abstract translation: 要解决的问题：通过引入导致酶的热稳定性的新突变来提供T7RNA聚合酶的改进变体。 解决方案：提供T7RNA聚合酶的变体，通过在Val426，Ser633，Val650，Thr654，Ala702，Val795及其组合的位置引入新的突变来提高热稳定性。 版权所有（C）2012，JPO＆INPIT
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公开(公告)号：JP2003199592A
公开(公告)日：2003-07-15
申请号：JP2002351736
申请日：2002-12-03
Applicant: HOFFMANN LA ROCHE
Inventor： SOBEK HARALD , GREIF MICHAEL
Abstract: PROBLEM TO BE SOLVED: To provide a method for amplifying a nucleic acid, by using an amplification reaction for which a primer is used, and to provide a reagent (composition) used in the method. SOLUTION: This composition contains a first modified thermostable enzyme which exhibits 3'-exsonuclease activity but does not substantially exhibit DNA polymerase activity and a second modified thermostable enzyme which exhibits the DNA polymerase activity, wherein the composition is enhanced in enzyme activity, when the enzymes are incubated in an aqueous buffer at an alkaline pH and a temperature of >50°C, so that a primer elongation product is formed. The method for amplifying the target nucleic acid comprises a process (a) where a mixture for the amplification reaction which comprises a primer complementary to the target nucleic acid, deoxynucleotide or its derivative, and the composition is made to contact with a sample and another process (b) where the sample and the mixture for the amplification reaction which are obtained in the process (a) are incubated for such a time at the temperature of >50°C that is enough for reactivating the first and second modified thermostable enzymes and forming the primer elongation product. COPYRIGHT: (C)2003,JPO
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Patent Agency Ranking
公开(公告)号：JP2002315584A
公开(公告)日：2002-10-29
申请号：JP2001289857
申请日：2001-09-21
Applicant: HOFFMANN LA ROCHE
Inventor： SOBEK HARALD , MUELLER RAINER , SCHMIDT MANFRED , FREI BRUNO , SUPPMANN BERNHARD , SCHMUCK RAINER , THALHOFER JOHANN-PETER , PALLUA PETER , PAJATSCH MARKUS
Abstract: PROBLEM TO BE SOLVED: To provide a sufficient amount of a recombinant active heterodimer- AMV-RT. SOLUTION: An active heterodimer-AMV-RT is produced in a prokaryotic host cell by (i) cloning one or more DNA sequences encoding the α-chain and/or the β-chain of AMV-RT to an expression plasmid, (ii) transforming the expressed plasmid to a prokaryotic cell, (iii) inducing a soluble expression of heterodimer-AMV-RT and (iv) isolating the recombinant heterodimer-AMV- RT from the cell.
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公开(公告)号：JP2015091261A
公开(公告)日：2015-05-14
申请号：JP2015003548
申请日：2015-01-09
Applicant: HOFFMANN LA ROCHE
Inventor： HASLINGER TOBIAS , METZLER THOMAS DR , PECENY ANNETTE , SOBEK HARALD
IPC: C12N15/09
Abstract: 【課題】一定の保存時間の後の再溶解の際に化合物の生体活性を維持する反応化合物の乾燥組成物を得るための方法の提供。【解決手段】反応化合物の保存可能な乾燥組成物を製造する方法であって、前記方法がa) 反応化合物の液体混合物であって、プライマー、ヌクレオチド、Taq DNAポリメラーゼ及び第一安定化分子を含んで成る液体混合物を準備する工程、及びb) 前記液体混合物の周囲の圧力を減少させることで、前記液体混合物を乾燥する工程、を含んで成り、ここで、前記反応化合物の乾燥組成物は水溶液中で可溶であり、工程a)における前記反応化合物の液体混合物は、第二安定化分子として、アプタマーを更に含んで成ることを特徴とする、方法。【選択図】図1
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公开(公告)号：CA2911965C
公开(公告)日：2018-06-19
申请号：CA2911965
申请日：2014-07-03
Applicant: HOFFMANN LA ROCHE
Inventor： CZABANY TIBOR , ENGEL ALFRED , GREIF MICHAEL , JUNG CHRISTINE , LULEY CHRISTIANE , MALIK SEBASTIAN , MUELLER RAINER , NIDETZKY BERND , RIBITSCH DORIS , SCHMOELZER KATHARINA , SCHWAB HELMUT , SOBEK HARALD , SUPPMANN BERNHARD , THOMANN MARCO , ZITZENBACHER SABINE
Abstract: The present disclosure is directed to the use of certain glycosyltransferase variants having N-terminal truncation deletions. It was found that the combination of two different truncation variants of human ß-galactoside-a-2,6-sialyltransferase I (hST6Gal-I) exhibited different specific sialyltransferase enzymatic activities. In one example, under conditions wherein the first variant ?89 hST6Gal-I catalyzed formation of bi-sialylated target molecules the second variant ?108 hST6Gal-I catalyzed formation of mono-sialylated target molecules. Thus, disclosed are variants of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variants of mammalian glycosyltransferase and use thereof, particularly for sialylating in a quantitatively controlled manner terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins.
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公开(公告)号：HK1221263A1
公开(公告)日：2017-05-26
申请号：HK16109333
申请日：2016-08-05
Applicant: HOFFMANN LA ROCHE
Inventor： ENGEL ALFRED , GREIF MICHAEL , JUNG CHRISTINE , MALIK SEBASTIAN , MUELLER RAINER , SOBEK HARALD , SUPPMANN BERNHARD , THOMANN MARCO
IPC: C12P20060101 , A61K20060101
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公开(公告)号：CA2911965A1
公开(公告)日：2015-01-08
申请号：CA2911965
申请日：2014-07-03
Applicant: HOFFMANN LA ROCHE
Inventor： CZABANY TIBOR , ENGEL ALFRED , GREIF MICHAEL , JUNG CHRISTINE , LULEY CHRISTIANE , MALIK SEBASTIAN , MUELLER RAINER , NIDETZKY BERND , RIBITSCH DORIS , SCHMOELZER KATHARINA , SCHWAB HELMUT , SOBEK HARALD , SUPPMANN BERNHARD , THOMANN MARCO , ZITZENBACHER SABINE
Abstract: The present disclosure is directed to the use of certain glycosyltransferase variants having N-terminal truncation deletions. It was found that the combination of two different truncation variants of human ß-galactoside-a-2,6-sialyltransferase I (hST6Gal-I) exhibited different specific sialyltransferase enzymatic activities. In one example, under conditions wherein the first variant ?89 hST6Gal-I catalyzed formation of bi-sialylated target molecules the second variant ?108 hST6Gal-I catalyzed formation of mono-sialylated target molecules. Thus, disclosed are variants of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variants of mammalian glycosyltransferase and use thereof, particularly for sialylating in a quantitatively controlled manner terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins.
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