公开(公告)号：JP2002315584A

公开(公告)日：2002-10-29

申请号：JP2001289857

申请日：2001-09-21

Applicant: HOFFMANN LA ROCHE

Inventor： SOBEK HARALD ,  MUELLER RAINER ,  SCHMIDT MANFRED ,  FREI BRUNO ,  SUPPMANN BERNHARD ,  SCHMUCK RAINER ,  THALHOFER JOHANN-PETER ,  PALLUA PETER ,  PAJATSCH MARKUS

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54

Abstract: PROBLEM TO BE SOLVED: To provide a sufficient amount of a recombinant active heterodimer- AMV-RT. SOLUTION: An active heterodimer-AMV-RT is produced in a prokaryotic host cell by (i) cloning one or more DNA sequences encoding the α-chain and/or the β-chain of AMV-RT to an expression plasmid, (ii) transforming the expressed plasmid to a prokaryotic cell, (iii) inducing a soluble expression of heterodimer-AMV-RT and (iv) isolating the recombinant heterodimer-AMV- RT from the cell.

公开(公告)号：JP2003304889A

公开(公告)日：2003-10-28

申请号：JP2003099615

申请日：2003-04-02

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲー

Inventor： MUELLER RAINER ,  PAJATSCH MARKUS ,  CURDT INGO ,  SOBEK HARALD DR ,  SCHMIDT MANFRED ,  SUPPMANN BERNHARD ,  SONN KIRSTEN ,  SCHNEIDINGER BERND

IPC: C12N15/09 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/48

CPC classification number: C12N9/1264 ,  C12Y207/07031 ,  G01N2510/00

Abstract: PROBLEM TO BE SOLVED: To provide a terminal deoxynucleotidyl transferase having uniform state with sufficient enzymatic activity. SOLUTION: The transferase is a truncated TdT derivative wherein at most 161 amino acids at the N-terminal are truncated and exhibiting enzymatic activity in a solution containing Co 2+ ion corresponding to 20-30 times the enzymatic activity of natural terminal deoxynucleotidyl transferase (TdT). COPYRIGHT: (C)2004,JPO

Abstract translation: 要解决的问题：提供具有均匀状态的末端脱氧核苷酸转移酶，具有足够的酶活性。 解决方案：转移酶是截短的TdT衍生物，其N-末端的至多161个氨基酸被截短并且在含有对应于20-30倍的Co 2 + 离子的溶液中表现出酶活性 天然末端脱氧核苷酸转移酶（TdT）的酶活性。 版权所有（C）2004，JPO

公开(公告)号：CA2357540C

公开(公告)日：2010-07-13

申请号：CA2357540

申请日：2001-09-20

Applicant: HOFFMANN LA ROCHE

Inventor： SOBEK HARALD ,  MUELLER RAINER ,  SCHMIDT MANFRED ,  FREY BRUNO ,  SUPPMANN BERNHARD ,  SCHMUCK RAINER ,  THALHOFER JOHANN-PETER ,  PALLUA PETER ,  PAJATSCH MARKUS

IPC: C12N15/09 ,  C12N15/10 ,  C07H21/00 ,  C12N1/21 ,  C12N9/12 ,  C12N15/52 ,  C12N15/54 ,  C12P19/34 ,  C12Q1/68

Abstract: The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.

公开(公告)号：AT471376T

公开(公告)日：2010-07-15

申请号：AT01121754

申请日：2001-09-19

Applicant: HOFFMANN LA ROCHE

Inventor： SOBEK HARALD ,  MUELLER RAINER ,  SCHMIDT MANFRED ,  FREY BRUNO ,  SUPPMANN BERNHARD ,  SCHMUCK RAINER ,  THALHOFER JOHANN-PETER ,  PALLUA PETER ,  PAJATSCH MARKUS

IPC: C12N15/09 ,  C12N15/54 ,  C12N1/21 ,  C12N9/12 ,  C12N15/70 ,  C12Q1/48

Abstract: The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.

公开(公告)号：CA2423887A1

公开(公告)日：2003-10-05

申请号：CA2423887

申请日：2003-04-01

Applicant: HOFFMANN LA ROCHE

Inventor： PAJATSCH MARKUS ,  CURDT INGO ,  SCHNEIDINGER BERND ,  SOBEK HARALD ,  SCHMIDT MANDFRED ,  SUPPMANN BERNHARD ,  MUELLER RAINER ,  SONN KIRSTEN

IPC: C12N15/09 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/48 ,  C12N9/10 ,  C07K19/00 ,  C12N15/62 ,  C12N15/63 ,  C12P21/02 ,  G01N33/573

Abstract: Truncated terminal deoxynucleotidyl transferase (TdT) derivative from calf thymus characterized in that the derivative in comparison to the native TdT is N- terminally truncated by up to 161 amino acids and has a 20- 30-fold higher enzyme activity in solutions containing Co~ions, and it's recombinant production and use.

公开(公告)号：CA2423887C

公开(公告)日：2008-01-22

申请号：CA2423887

申请日：2003-04-01

Applicant: HOFFMANN LA ROCHE

Inventor： PAJATSCH MARKUS ,  MUELLER RAINER ,  SOBEK HARALD ,  CURDT INGO ,  SONN KIRSTEN ,  SCHMIDT MANFRED ,  SUPPMANN BERNHARD ,  SCHNEIDINGER BERND

IPC: C12N9/12 ,  C12N15/09 ,  C12N9/10 ,  C12N15/54 ,  C12N15/63 ,  C12P19/34 ,  C12P21/02 ,  C12Q1/48 ,  G01N33/573

Abstract: Truncated terminal deoxynucleotidyl transferase (TdT) derivative from calf thymus characterized in that the derivative in comparison to the native TdT is N- terminally truncated by up to 161 amino acids and has a 20- 30-fold higher enzyme activity in solutions containing Co~ions, and it's recombinant production and use.

公开(公告)号：CA2357540A1

公开(公告)日：2002-03-22

申请号：CA2357540

申请日：2001-09-20

Applicant: HOFFMANN LA ROCHE

Inventor： THALHOFER JOHANN-PETER ,  SOBEK HARALD ,  SCHMUCK RAINER ,  PALLUA PETER ,  SCHMIDT MANFRED ,  MUELLER RAINER ,  FREY BRUNO ,  PAJATSCH MARKUS ,  SUPPMANN BERNHARD

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12N15/10 ,  C07H21/00 ,  C12P19/34 ,  C12N15/52 ,  C12Q1/68

Abstract: The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.