公开(公告)号：JP2011224002A

公开(公告)日：2011-11-10

申请号：JP2011090825

申请日：2011-04-15

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft

Inventor： GREIF MICHAEL ,  MUELLER RAINER ,  RUDOLPH CHRISTIAN ,  RUF ARMIN ,  SCHMIDT MANFRED ,  SOBEK HARALD ,  THALHOFER JOHANN-PETER

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12P19/34

CPC classification number: C12N9/1247 ,  C12P19/34 ,  C12Y207/07006

Abstract: PROBLEM TO BE SOLVED: To provide an improved variant of T7 RNA polymerase by introducing new mutation leading to improved thermostability of the enzyme.SOLUTION: There is provided a variant of T7 RNA polymerase leading to improved thermostability by introducing a new mutation at positions Val426, Ser633, Val650, Thr654, Ala702, Val795 and combinations thereof.

Abstract translation: 要解决的问题：通过引入导致酶的热稳定性的新突变来提供T7RNA聚合酶的改进变体。 解决方案：提供T7RNA聚合酶的变体，通过在Val426，Ser633，Val650，Thr654，Ala702，Val795及其组合的位置引入新的突变来提高热稳定性。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2010252793A

公开(公告)日：2010-11-11

申请号：JP2010099666

申请日：2010-04-23

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft

Inventor： HOELKE WERNER ,  MUELLER RAINER ,  SCHMIDT MANFRED ,  SOBEK HARALD ,  THALHOFER JOHANN-PETER

IPC: C12N9/96 ,  C12N9/24 ,  C12N15/09

CPC classification number: C07K14/435

Abstract: PROBLEM TO BE SOLVED: To provide an aqueous composition containing a protein having enzymatic activity of an alpha-galactosidase.
SOLUTION: This method for stabilizing the aqueous composition containing the protein having the enzymatic activity of the alpha-galactosidase is provided. The method for preparing the purified aqueous composition containing the protein having the enzymatic activity of the alpha-galactosidase is also provided.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 待解决的问题：提供含有具有α-半乳糖苷酶的酶活性的蛋白质的含水组合物。 解决方案：提供了用于稳定含有具有α-半乳糖苷酶的酶活性的蛋白质的含水组合物的方法。 还提供了制备含有具有α-半乳糖苷酶的酶活性的蛋白质的纯化含水组合物的方法。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2002315584A

公开(公告)日：2002-10-29

申请号：JP2001289857

申请日：2001-09-21

Applicant: HOFFMANN LA ROCHE

Inventor： SOBEK HARALD ,  MUELLER RAINER ,  SCHMIDT MANFRED ,  FREI BRUNO ,  SUPPMANN BERNHARD ,  SCHMUCK RAINER ,  THALHOFER JOHANN-PETER ,  PALLUA PETER ,  PAJATSCH MARKUS

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54

Abstract: PROBLEM TO BE SOLVED: To provide a sufficient amount of a recombinant active heterodimer- AMV-RT. SOLUTION: An active heterodimer-AMV-RT is produced in a prokaryotic host cell by (i) cloning one or more DNA sequences encoding the α-chain and/or the β-chain of AMV-RT to an expression plasmid, (ii) transforming the expressed plasmid to a prokaryotic cell, (iii) inducing a soluble expression of heterodimer-AMV-RT and (iv) isolating the recombinant heterodimer-AMV- RT from the cell.

公开(公告)号：JP2003304889A

公开(公告)日：2003-10-28

申请号：JP2003099615

申请日：2003-04-02

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲー

Inventor： MUELLER RAINER ,  PAJATSCH MARKUS ,  CURDT INGO ,  SOBEK HARALD DR ,  SCHMIDT MANFRED ,  SUPPMANN BERNHARD ,  SONN KIRSTEN ,  SCHNEIDINGER BERND

IPC: C12N15/09 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/48

CPC classification number: C12N9/1264 ,  C12Y207/07031 ,  G01N2510/00

Abstract: PROBLEM TO BE SOLVED: To provide a terminal deoxynucleotidyl transferase having uniform state with sufficient enzymatic activity. SOLUTION: The transferase is a truncated TdT derivative wherein at most 161 amino acids at the N-terminal are truncated and exhibiting enzymatic activity in a solution containing Co 2+ ion corresponding to 20-30 times the enzymatic activity of natural terminal deoxynucleotidyl transferase (TdT). COPYRIGHT: (C)2004,JPO

Abstract translation: 要解决的问题：提供具有均匀状态的末端脱氧核苷酸转移酶，具有足够的酶活性。 解决方案：转移酶是截短的TdT衍生物，其N-末端的至多161个氨基酸被截短并且在含有对应于20-30倍的Co 2 + 离子的溶液中表现出酶活性 天然末端脱氧核苷酸转移酶（TdT）的酶活性。 版权所有（C）2004，JPO

公开(公告)号：ES2538694T3

公开(公告)日：2015-06-23

申请号：ES12162102

申请日：2012-03-29

Applicant: HOFFMANN LA ROCHE

Inventor： GREIF MICHAEL ,  RUDOLPH CHRISTIAN ,  SOBEK HARALD ,  THALHOFER JOHANN-PETER ,  SCHMIDT MANFRED

IPC: C12N9/12 ,  C12Q1/68

Abstract: Una solución acuosa en la que está ausente un agente reductor con un grupo tiol, comprendiendo la solución acuosa un polipéptido, comprendiendo el polipéptido una variante de la T7 ARN polimerasa (variante de T7), teniendo la variante de T7 las propiedades de (i) actividad de ARN polimerasa dependiente de ADN, y (ii) una composición distinta de aminoácidos en comparación con el polipéptido de 883 aminoácidos de la T7 ARN polimerasa de SEC ID Nº: 2 (referencia de tipo silvestre), en la que en una posición seleccionada de la 510 a la 530 de la variante de T7, numerada a partir del extremo N-terminal de la referencia de tipo silvestre, está presente un resto de Cisteína, en la que la variante de T7 comprende una o más sustituciones de aminoácidos de las cuales una (o la primera) está en la posición 723 y donde la Serina sustituye al resto de Cisteína (Cys723Ser), y en la que está ausente la formación de homomultímeros en la solución acuosa de la variante de T7 como resultado de uno o más enlaces disulfuro intermoleculares.

公开(公告)号：CA2737014A1

公开(公告)日：2011-10-16

申请号：CA2737014

申请日：2011-04-12

Applicant: HOFFMANN LA ROCHE

Inventor： GREIF MICHAEL ,  MUELLER RAINER ,  RUDOLPH CHRISTIAN ,  RUF ARMIN ,  SCHMIDT MANFRED ,  SOBEK HARALD ,  THALHOFER JOHANN-PETER

IPC: C12N15/54 ,  C12N9/12 ,  C12N15/00 ,  C12P19/34

Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.

公开(公告)号：CA2697999C

公开(公告)日：2013-04-30

申请号：CA2697999

申请日：2010-03-26

Applicant: HOFFMANN LA ROCHE

Inventor： HOELKE WERNER ,  MUELLER RAINER ,  SCHMIDT MANFRED ,  SOBEK HARALD ,  THALHOFER JOHANN-PETER

IPC: C12N9/40 ,  C12N9/96

Abstract: The present invention provides an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase. The present invention further provides a method of stabilizing an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase, and a method of preparing a purified aqueous composition comprising said protein with enzymatic activity of alpha-galactosidase.

公开(公告)号：HK1163165A1

公开(公告)日：2012-09-07

申请号：HK12103705

申请日：2012-04-16

Applicant: HOFFMANN LA ROCHE

Inventor： GREIF MICHAEL ,  MUELLER RAINER ,  RUDOLPH CHRISTIAN ,  RUF ARMIN ,  SCHMIDT MANFRED ,  SOBEK HARALD ,  THALHOFER JOHANN-PETER

IPC: C12N20060101 ,  C12R20060101

公开(公告)号：CA2357540C

公开(公告)日：2010-07-13

申请号：CA2357540

申请日：2001-09-20

Applicant: HOFFMANN LA ROCHE

Inventor： SOBEK HARALD ,  MUELLER RAINER ,  SCHMIDT MANFRED ,  FREY BRUNO ,  SUPPMANN BERNHARD ,  SCHMUCK RAINER ,  THALHOFER JOHANN-PETER ,  PALLUA PETER ,  PAJATSCH MARKUS

IPC: C12N15/09 ,  C12N15/10 ,  C07H21/00 ,  C12N1/21 ,  C12N9/12 ,  C12N15/52 ,  C12N15/54 ,  C12P19/34 ,  C12Q1/68

Abstract: The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.

公开(公告)号：AT317898T

公开(公告)日：2006-03-15

申请号：AT03006965

申请日：2003-03-27

Applicant: HOFFMANN LA ROCHE

Inventor： MUELLER RAINER DR ,  PAJATSCH MARKUS DR ,  CURDT INGO DR ,  SOBEK HARALD DR ,  SCHMIDT MANFRED ,  SUPPMANN BERNHARD DR ,  SONN KIRSTEN ,  SCHNEIDINGER BERND DR

IPC: C12N15/09 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/48