公开(公告)号：JP2004201688A

公开(公告)日：2004-07-22

申请号：JP2003422697

申请日：2003-12-19

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft

Inventor： MUELLER RAINER ,  KIRSCHBAUM THOMAS ,  SUPPMANN BERNHARD ,  SCHOEN HELMUT ,  ENGH RICHARD ,  HOFFMANN ARTUR ,  THALHOFER JOHANN-PETER ,  SIEDEL JOACHIM ,  ENGEL WOLF-DIETER

IPC: C12N15/09 ,  C12N1/16 ,  C12N1/19 ,  C12N9/16 ,  C12N9/22 ,  C12N15/55 ,  C12N15/63 ,  C12Q1/34 ,  C12R1/84

CPC classification number: C12N9/22

Abstract: PROBLEM TO BE SOLVED: To provide desoxyribonuclease with increased thermolability compared to bovine pancreatic desoxyribonuclease I, and to provide a method with a high cost-efficiency, for producing the desoxyribonuclease by non-animal host organisms. SOLUTION: The bovine pancreatic desoxyribonuclease I variant has a specific amino acid residue of the bovine pancreatic desoxyribonuclease I substituted with a different amino acid, and has activity of the desoxyribonuclease I. The method for producing the same uses a base sequence encoding the variant. A Pichia pastoris strain encodes a preprotein comprising the variant and a signal peptide, and contains a Pichia pastoris AOX1 promoter or a specific base sequence connected to the promoter element, wherein the base sequence encoding the preprotein contains a chromosome obtained by carrying out fusion of the base sequence of the bovine pancreatic desoxyribonuclease I. COPYRIGHT: (C)2004,JPO&NCIPI

公开(公告)号：JP2004194657A

公开(公告)日：2004-07-15

申请号：JP2003419939

申请日：2003-12-17

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft

Inventor： MUELLER RAINER ,  SUPPMANN BERNHARD ,  THALHOFER JOHANN-PETER ,  RONNING STEPHANIE ,  SCHOEN HELMUT

IPC: C12N15/09 ,  C12N9/22 ,  C12N9/88 ,  C12N15/55 ,  C12N15/81 ,  C12P19/34 ,  C12R1/72

CPC classification number: Y02P20/52

Abstract: PROBLEM TO BE SOLVED: To provide a substitutional supply source for bovine pancreatic deoxyribonulcease I, having a high cost effect. SOLUTION: A method for producing a bovine pancreatic protein includes (a) a process for providing a vector containing a nucleotide sequence which encodes a preprotein comprising the bovine pancreatic protein and a signal peptide, (b) another process for transforming a methylotrophic yeast strain by the vector, (c) a third process for culturing the transformed methylotrophic yeast strain in a growth medium containing nutrients and methanol, wherein the methylotrophic yeast strain expresses the bovine pancreatic protein and secretes the protein in the growth medium, and (d) the other process for purifying the bovine pancreatic protein from the growth medium, so that the bovine pancreatic protein purified in the process (d) has a deoxyribonulcease specific activity of at least 6,000 units per mg of the purified protein. COPYRIGHT: (C)2004,JPO&NCIPI

公开(公告)号：JP2009297028A

公开(公告)日：2009-12-24

申请号：JP2009139667

申请日：2009-06-10

Applicant: F Hoffmann-La Roche Ag ,  エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト

Inventor： HOELKE WERNER ,  HOFFMANN ARTUR ,  MARX THOMAS ,  SONN KIRSTEN ,  SUPPMANN BERNHARD ,  THALHOFER JOHANN-PETER

IPC: C12N1/20 ,  C12N9/52

CPC classification number: C12N1/20 ,  C12N9/52

Abstract: PROBLEM TO BE SOLVED: To provide an improved medium for culturing Clostridium histolyticum and culture supernatants for biotechnologically producing collagenase enzymes.
SOLUTION: The culture media contain water, fish gelatin and peptones derived from a non-mammalian source. The media preferably contain peptones derived from plants as a non-mammalian source. The media also may contain two or more different peptones. Disclosed are sterilized compositions comprising the media, a culture supernatant containing the collagenase of Clostridium histolyticum, a method for producing the culture supernatant, and a method for producing the collagenase.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供用于培养溶组织梭菌和用于生物技术生产胶原酶的培养上清液的改进培养基。 解决方案：培养基含有源自非哺乳动物来源的水，鱼明胶和胨。 培养基优选含有源自植物的胨作为非哺乳动物来源。 媒体也可能含有两种或更多种不同的蛋白胨。 公开了包含培养基的灭菌组合物，含有溶组织梭菌胶原酶的培养上清液，培养上清液的制备方法和胶原酶的制备方法。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2003304889A

公开(公告)日：2003-10-28

申请号：JP2003099615

申请日：2003-04-02

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲー

Inventor： MUELLER RAINER ,  PAJATSCH MARKUS ,  CURDT INGO ,  SOBEK HARALD DR ,  SCHMIDT MANFRED ,  SUPPMANN BERNHARD ,  SONN KIRSTEN ,  SCHNEIDINGER BERND

IPC: C12N15/09 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/48

CPC classification number: C12N9/1264 ,  C12Y207/07031 ,  G01N2510/00

Abstract: PROBLEM TO BE SOLVED: To provide a terminal deoxynucleotidyl transferase having uniform state with sufficient enzymatic activity. SOLUTION: The transferase is a truncated TdT derivative wherein at most 161 amino acids at the N-terminal are truncated and exhibiting enzymatic activity in a solution containing Co 2+ ion corresponding to 20-30 times the enzymatic activity of natural terminal deoxynucleotidyl transferase (TdT). COPYRIGHT: (C)2004,JPO

Abstract translation: 要解决的问题：提供具有均匀状态的末端脱氧核苷酸转移酶，具有足够的酶活性。 解决方案：转移酶是截短的TdT衍生物，其N-末端的至多161个氨基酸被截短并且在含有对应于20-30倍的Co 2 + 离子的溶液中表现出酶活性 天然末端脱氧核苷酸转移酶（TdT）的酶活性。 版权所有（C）2004，JPO

公开(公告)号：JP2009291195A

公开(公告)日：2009-12-17

申请号：JP2009133447

申请日：2009-06-02

Applicant: F Hoffmann-La Roche Ag ,  エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト

Inventor： ECKSTEIN HELLMUT ,  FISCHER MICHAELA ,  HOELKE WERNER ,  LIEHRE ANTJE ,  SUPPMANN BERNHARD ,  THALHOFER JOHANN-PETER

IPC: C12N9/52 ,  C07K1/18 ,  C07K1/20

CPC classification number: C12N9/52 ,  C07K14/33

Abstract: PROBLEM TO BE SOLVED: To provide a method for purifying Clostridium histolyticum collagenase type I and type II proteins from a complex mixture. SOLUTION: The method for purifying Clostridium histolyticum collagenase includes carrying out the purification by performing precipitation with ammonium sulfate, hydrophobic interaction chromatography, cation exchange chromatography, and anion chromatography. Conditions are provided which lead to a stabilized partially purified preparation and the precipitation step. The preparations provide exceptionally pure and intact collagenase type I and type II proteins which are enzymatically active. The blends of the two isolated proteins are provided. The use of the purified collagenase proteins or blends thereof for treating a tissue sample in vitro are also provided. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 待解决的问题：提供从复合混合物中纯化溶组织梭状芽胞杆菌胶原酶I型和II型蛋白的方法。 解决方案：用于纯化溶组织梭菌胶原酶的方法包括通过用硫酸铵进行沉淀，疏水相互作用层析，阳离子交换层析和阴离子色谱法进行纯化。 提供了导致稳定的部分纯化制剂和沉淀步骤的条件。 该制剂提供非常纯的和完整的胶原酶I型和II型蛋白质，其具有酶促活性。 提供了两种分离的蛋白质的共混物。 还提供了纯化的胶原酶蛋白或其混合物用于在体外处理组织样品的用途。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2002315584A

公开(公告)日：2002-10-29

申请号：JP2001289857

申请日：2001-09-21

Applicant: HOFFMANN LA ROCHE

Inventor： SOBEK HARALD ,  MUELLER RAINER ,  SCHMIDT MANFRED ,  FREI BRUNO ,  SUPPMANN BERNHARD ,  SCHMUCK RAINER ,  THALHOFER JOHANN-PETER ,  PALLUA PETER ,  PAJATSCH MARKUS

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54

Abstract: PROBLEM TO BE SOLVED: To provide a sufficient amount of a recombinant active heterodimer- AMV-RT. SOLUTION: An active heterodimer-AMV-RT is produced in a prokaryotic host cell by (i) cloning one or more DNA sequences encoding the α-chain and/or the β-chain of AMV-RT to an expression plasmid, (ii) transforming the expressed plasmid to a prokaryotic cell, (iii) inducing a soluble expression of heterodimer-AMV-RT and (iv) isolating the recombinant heterodimer-AMV- RT from the cell.

公开(公告)号：CA2908407C

公开(公告)日：2022-06-14

申请号：CA2908407

申请日：2014-05-16

Applicant: HOFFMANN LA ROCHE

Inventor： ENGEL ALFRED ,  GREIF MICHAEL ,  JUNG CHRISTINE ,  MALIK SEBASTIAN ,  MUELLER RAINER ,  SOBEK HARALD ,  SUPPMANN BERNHARD ,  THOMANN MARCO

IPC: C12P21/00 ,  A61K39/395 ,  C12P19/18

Abstract: The present disclosure is directed to the use of certain glycosyltransferase variants having N-terminal truncation deletions. Contrary to previous findings certain truncations were found to exhibit sialidase enzymatic activity, particularly a variant of human sialyltransferase (hST6Gal-I) with a truncation deletion involving the first 89 N-terminal amino acids of the respective wild-type polypeptide. A fundamental finding documented in the present disclosure is that there exists a variant of this enzyme which is capable of catalyzing transfer of a glycosyl moiety as well as hydrolysis thereof. Thus, disclosed is a specific exemplary variant of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variant of mammalian glycosyltransferase and use thereof, particularly for sialylating in a quantitatively controlled manner terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins.

公开(公告)号：CA2668353C

公开(公告)日：2017-11-07

申请号：CA2668353

申请日：2009-06-08

Applicant: HOFFMANN LA ROCHE

Inventor： WERNER HOELKE ,  HOFFMANN ARTUR ,  MARX THOMAS ,  SUPPMANN BERNHARD ,  THALHOFER JOHANN-PETER ,  SONN KIRSTEN

IPC: C12N1/20 ,  C12N9/52

Abstract: The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferablyplant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.

公开(公告)号：CA2668118C

公开(公告)日：2016-09-13

申请号：CA2668118

申请日：2009-06-01

Applicant: HOFFMANN LA ROCHE

Inventor： ECKSTEIN HELLMUT ,  FISCHER MICHAELA ,  HOELKE WERNER ,  LIEHRE ANTJE ,  SUPPMANN BERNHARD ,  THALHOFER JOHANN-PETER

IPC: C12N9/52 ,  C07K1/14 ,  C12N5/00 ,  C12N9/48 ,  C12Q1/00 ,  G01N1/28

Abstract: The present invention provides a method for purifying Clostridium histolyticum collagenase type I and type II proteins from a complex mixture by subsequently performing a precipitation with ammonium sulfate, hydrophobic interaction chromatography, cation exchange chromatography, and anion chromatography. Conditions are provided which lead to a stabilized partially purified preparation even after the precipitation step. The method of the invention leads to a quick and efficient removal of other proteolytic activities. The preparations according to the invention provide exceptionally pure and intact collagenase type I and type II proteins which are enzymatically active. The invention also provides blends of the two isolated proteins. The invention further provides the use of the purified collagenase proteins or blends thereof for treating a tissue sample in vitro.

公开(公告)号：CA2357540C

公开(公告)日：2010-07-13

申请号：CA2357540

申请日：2001-09-20

Applicant: HOFFMANN LA ROCHE

Inventor： SOBEK HARALD ,  MUELLER RAINER ,  SCHMIDT MANFRED ,  FREY BRUNO ,  SUPPMANN BERNHARD ,  SCHMUCK RAINER ,  THALHOFER JOHANN-PETER ,  PALLUA PETER ,  PAJATSCH MARKUS

IPC: C12N15/09 ,  C12N15/10 ,  C07H21/00 ,  C12N1/21 ,  C12N9/12 ,  C12N15/52 ,  C12N15/54 ,  C12P19/34 ,  C12Q1/68

Abstract: The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.