公开(公告)号：EP1306438A4

公开(公告)日：2005-03-02

申请号：EP01956776

申请日：2001-08-01

Applicant: KANEKA CORP

Inventor： KIZAKI NORIYUKI ,  YASOHARA YOSHIHIKO ,  HASEGAWA JUNZO

IPC: C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/53 ,  C12P17/10 ,  C12P41/00

CPC classification number: C12N9/0008 ,  C12N9/0006 ,  C12P17/10 ,  C12P41/002

Abstract: Novel polypeptides capable of forming (S)-N-benzyl-3-pyrrolidinol, DNA encoding the same and a method of using the same. A polypeptide having the following physicochemical properties (1) to (5): (1) function: asymmetrically reducing N-benzyl-3-pyrrolidinone by using NADPH as a coenzyme to form (S)-N-benzyl-3-pyrodinol; (2) optimum functional pH: 4.5 to 5.5; (3) optimum functional temperature: 40 to 45°C; (4) molecular weight: about 29,000 in gel filtration analysis, about 35,000 in SDS polyacrylamide electrophoresis; and (5) inhibitor: being inhibited by divalent copper ion. A polypeptide having the amino acid sequence represented by SEQ ID NO:1, or a polypeptide having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:1 by the substitution, insertion, deletion and/or addition of one or more amino acids and having an enzymatic activity of asymmetrically reducing N-benzyl-3-pyrrolidinone to form (S)-N-benzyl-3-pyrrolidinol.

公开(公告)号：EP0967271A4

公开(公告)日：2002-09-25

申请号：EP97937861

申请日：1997-09-01

Applicant: KANEKA CORP

Inventor： YASOHARA YOSHIHIKO ,  KIZAKI NORIYUKI ,  HASEGAWA JUNZO ,  WADA MASARU ,  SHIMIZU SAKAYU ,  KATAOKA MICHIHIKO ,  YAMAMOTO K ,  KAWABATA HIROSHI ,  KITA KEIKO

IPC: C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/53 ,  C12N15/63 ,  C12P7/62 ,  C12P41/00 ,  C12R1/91

CPC classification number: C12P7/62 ,  C12N9/0006 ,  C12P41/002

Abstract: An enzyme having the activity of asymmetrically reducing a carbonyl compound to an optically active alcohol, a DNA which encodes the enzyme, a plasmid having the DNA, cells transformed by the plasmid, and a process for producing optically active alcohols with the enzyme and/or the transformed cells.

公开(公告)号：EP1123979A4

公开(公告)日：2002-03-27

申请号：EP00954944

申请日：2000-08-24

Applicant: KANEKA CORP

Inventor： MATSUDA HIDEYUKI ,  KAWAMUKAI MAKOTO ,  YAJIMA KAZUYOSHI ,  IKENAKA YASUHIRO ,  HASEGAWA JUNZO ,  TAKAHASHI SATOMI

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/12 ,  C12N15/54 ,  C12P7/66 ,  C12R1/19

CPC classification number: C12N9/1085 ,  C12P7/66

Abstract: A process for microbiologically producing coenzyme Q10 at a high efficiency by using a gene of the synthesis of coenzyme Q10 side chain originating in a fungus belonging to the genus Saitoella. A DNA having the base sequence represented by SEQ ID NO:1; a DNA having a base sequence derived from the base sequence represented by SEQ ID NO:1 by deletion, addition, insertion and/or substitution of one or more bases and encoding a protein having a decaprenyl diphosphate synthase activity; and a DNA being hybridizable under stringent conditions with the DNA comprising the base sequence represented by SEQ ID NO:1 and encoding a protein having a decaprenyl diphosphate synthase activity.

公开(公告)号：EP0902011A4

公开(公告)日：2002-03-20

申请号：EP98902203

申请日：1998-02-13

Applicant: KANEKA CORP

Inventor： YAMADA MASAHIKO ,  NAGASHIMA NOBUO ,  HASEGAWA JUNZO

IPC: B01J23/44 ,  C07B53/00 ,  C07B61/00 ,  C07C227/08 ,  C07C227/18 ,  C07C229/36 ,  C07C253/30 ,  C07C255/58 ,  C07C319/20 ,  C07C323/63

CPC classification number: C07C227/18 ,  C07C227/08 ,  C07C229/36

Abstract: A highly economical and efficient process for producing optically active homophenylalanine derivatives represented by general formula (IV); and intermediates therefor and a process for producing the same. The process comprises reacting a β-benzoylacrylic acid derivative represented by general formula (II) with a 1-arylethylamine derivative represented by general formula (III) and reducing the resultant η-oxo-homophenylalanine derivative represented by general formula (I).

公开(公告)号：DE69841075D1

公开(公告)日：2009-10-01

申请号：DE69841075

申请日：1998-04-20

Applicant: KANEKA CORP

Inventor： YAMADA YUKIO ,  IWASAKI AKIRA ,  KIZAKI NORIYUKI ,  MATSUMOTO KEIJI ,  IKENAKA YASUHIRO ,  OGURA MASAHIRO ,  HASEGAWA JUNZO

IPC: C12P13/04 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12P13/00

Abstract: A process for efficiently producing optically active amino compounds, mainly (R)-amino compounds, at a low cost by means of a microbial enzyme; a polypeptide suitable for use in the production process and having a stereoselective transaminase activity; and a DNA coding for the polypeptide. The process is characterized by comprising causing a transaminase to act on a ketone compound as an amino acceptor in the presence of a primary amine as an amino donor to stereoselectively transfer the amino group to thus obtain an optically active amino compound. The DNA contains a base sequence coding for a polypeptide having a stereoselective transaminase activity. The polypeptide can be obtained by incubating a microorganism belonging to the genus Arthrobacter.

公开(公告)号：AT399208T

公开(公告)日：2008-07-15

申请号：AT01956776

申请日：2001-08-01

Applicant: KANEKA CORP

Inventor： KIZAKI NORIYUKI ,  YASOHARA YOSHIHIKO ,  HASEGAWA JUNZO

IPC: C12N15/53 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12P17/10 ,  C12P41/00

Abstract: The present invention provides a novel polypeptide producing (S)-N-benzyl-3-pyrrolidinol, a DNA coding for it and a method of using them. A polypeptide having the following physicochemical properties (1) to (5): (1) Action: It asymmetrically reduces N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol with NADPH as a coenzyme; (2) Optimum action pH: 4.5 to 5.5; (3) Optimum action temperature: 40 DEG C to 45 DEG C; (4) Molecular weight: About 29,000 as determined by gel filtration analysis, about 35,000 as determined by SDS-polyacrylamide gel electrophoresis analysis; (5) Inhibitor: It is inhibited by the divalent copper ion. Further, a polypeptide having the amino acid sequence shown under SEQ ID NO:1 in the sequence listing; or a polypeptide having an amino acid sequence obtainable from the amino acid sequence shown under SEQ ID NO:1 in the sequence listing by substitution, insertion, deletion and/or addition of one or more amino acids and having enzyme activity in asymmetrically reducing N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol.

公开(公告)号：DE60038281D1

公开(公告)日：2008-04-24

申请号：DE60038281

申请日：2000-06-28

Applicant: KANEKA CORP

Inventor： KAWANO SHIGERU ,  HORIKAWA MIHO ,  YASOHARA YOSHIHIKO ,  HASEGAWA JUNZO

IPC: C12N9/02 ,  C12N1/21 ,  C12N15/53 ,  C12N15/63 ,  C12P17/12 ,  C12P17/18 ,  C12P41/00 ,  C12R1/11 ,  C12R1/19 ,  C12R1/72

Abstract: The present invention relates to a method of producing an optically active pyridineethanol derivative. More particularly, it relates to a method of producing an optically active polycyclic pyridineethanol derivative by causing an enzyme or enzyme source to act on polycyclic acetylpyridine derivatives. The present invention also relates to a novel enzyme which can be used in the production method mentioned above, a DNA coding for said enzyme, a recombinant vector having said DNA, and a transformant having said recombinant vector. The invention further relates to a method of producing an optically active polycyclic pyridineethanol derivative by causing the above novel enzyme or the above transformant to act on optically inactive polycyclic pyridineethanol derivatives.

公开(公告)号：DE60018909T2

公开(公告)日：2006-03-30

申请号：DE60018909

申请日：2000-11-24

Applicant: KANEKA CORP

Inventor： KIZAKI NORIYUKI ,  YAMADA YUKIO ,  YASOHARA YOSHIHIKO ,  HASEGAWA JUNZO

IPC: C12N9/02 ,  C12N1/00 ,  C12N1/21 ,  C12N9/04 ,  C12N15/53 ,  C12P7/62 ,  C12P41/00

Abstract: A novel polypeptide for producing tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate, a gene encoding the polypeptide, and a method of using the polypeptide, are provided. The polypeptide has an enzyme activity to asymmetrically reduce tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate to tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate. The polypeptide comprises the amino acid sequence represented by SEQ ID NO. 1 in the Sequence Listing, or the amino acid sequence having at least one amino acid substitution, insertion, deletion, or addition thereof.

公开(公告)号：DE69732570T2

公开(公告)日：2005-12-29

申请号：DE69732570

申请日：1997-08-01

Applicant: KANEKA CORP

Inventor： YASOHARA YOSHIHIKO ,  MIYAMOTO KENJI ,  KAWANO SHIGERU ,  HASEGAWA JUNZO

IPC: C07C303/30 ,  C07C309/64 ,  C07C309/66 ,  C07C309/73 ,  C07C327/32 ,  C07D317/24 ,  C07C309/72 ,  C07B53/00

Abstract: Novel and useful optical active 2-aralkyl-3-sulfonyloxy-1-propanol and 2-aralkyl-3-sulfonyloxypropionic acid are provided by using an optical active 2-aralkyl-3-acyloxy-1-propanol as a starting material. Furthermore, an optical active 2-aralkyl-3-thiopropionic acid, which is an important intermediate of enkephalinase inhibitor, is provided. According to the present invention, industrially useful optical active sulfonic acid ester derivatives can be provided.

公开(公告)号：ES2240205T3

公开(公告)日：2005-10-16

申请号：ES00977938

申请日：2000-11-24

Applicant: KANEKA CORP

Inventor： KIZAKI NORIYUKI ,  YAMADA YUKIO ,  YASOHARA YOSHIHIKO ,  HASEGAWA JUNZO

IPC: C12N1/00 ,  C12N9/04 ,  C12N15/53 ,  C12P7/62 ,  C12P41/00 ,  C12N9/02 ,  C12N1/21

Abstract: Un polinucleótido que codifica un polipéptido que tiene una actividad enzimática para reducir asimétricamente el (S)-6-cloro-5-hidroxi-3-oxohexanoato de terc-butilo a (3R, 5S)-6-cloro-3, 5-dihidroxihexanoato de terc-butilo, en el que el polinucleótido es seleccionado entre el grupo que consta de: (a) Polinucleótidos que codifican un polipéptido que comprenden la secuencia de aminoácidos representados por el Nº de ID de SEQ: 1 en el listado de secuencias; (b) Polinucleótidos que comprenden la secuencia de nucleótidos del Nº de ID de SEQ: 2 en el listado de secuencias; y (c) Polinucleótidos que hibridizan con las secuencias de nucleótidos representadas por el Nº de ID de SEQ: 2 en el listado de secuencias en condiciones severas.