公开(公告)号：WO2010055959A1

公开(公告)日：2010-05-20

申请号：PCT/KR2008/006638

申请日：2008-11-11

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  HWANG, Kyo Seon ,  KIM, Tae Song ,  KIM, Sang Kyung ,  LEE, Sang Myung ,  LEE, Yoon Sik

Inventor： HWANG, Kyo Seon ,  KIM, Tae Song ,  KIM, Sang Kyung ,  LEE, Sang Myung ,  LEE, Yoon Sik

IPC: G01N33/53

CPC classification number: G01N33/54373

Abstract: Provided is a protein detection system using a micro-cantilever and based on immune responses, wherein the micro-cantilever shows significantly improved sensitivity to allow detection of a trace amount of biomolecule. To the micro-cantilever, sandwich immunoassay is applied, and the sandwich immunoassay uses a polyclonal antibody or silica nanoparticles having a monoclonal antibody bound thereto, so that variations in the output signals of the cantilever are amplified and the detection sensitivity is significantly improved. The system enables detection of disease specific antigen at several femtomolar levels, and makes it possible to detect a trace amount of protein related to diseases, particularly to cancers, with ease.

Abstract translation: 提供了使用微悬臂梁并基于免疫应答的蛋白质检测系统，其中微悬臂梁显示显着改善的灵敏度以允许检测微量的生物分子。 对微悬臂梁进行夹心免疫测定，夹心免疫测定使用结合有单克隆抗体的多克隆抗体或二氧化硅纳米粒子，扩大悬臂输出信号的变化，检测灵敏度明显提高。 该系统能够以几个摩尔体积水平检测疾病特异性抗原，并且可以容易地检测与疾病，特别是癌症相关的微量蛋白质。

公开(公告)号：WO2006031072A1

公开(公告)日：2006-03-23

申请号：PCT/KR2005/003066

申请日：2005-09-15

Applicant: Korea Institute of Science and Technology ,  LEE, Jeong Hoon ,  KIM, Tae Song ,  YOON, Dae Sung ,  HWANG, Kyo Seon

Inventor： LEE, Jeong Hoon ,  KIM, Tae Song ,  YOON, Dae Sung ,  HWANG, Kyo Seon

IPC: G01N29/12

CPC classification number: G01N29/022 ,  B82Y30/00 ,  B82Y35/00 ,  G01N29/036 ,  G01N29/326 ,  G01N2291/0215 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0258 ,  G01N2291/0426 ,  G01N2291/0427 ,  Y10T436/143333

Abstract: Disclosed are system and method for measuring a bio-element capable of accurately detecting whether a bio-element such as protein, gene and the like is present in an atmosphere or vapor phase having controlled temperature and humidity thereof and measuring a content of the bio- element. According to an embodiment of the invention, the method comprises steps of preparing a cantilever sensor having a plurality of cantilevers; measuring a basis resonant frequency for the plurality of cantilevers; reacting the cantilevers with a sample including a bio-element; measuring resonant frequencies of the cantilevers after the reaction, in a closed system that is isolated from an exterior environment and temperature and humidity thereof are controlled to a specific state; and calculating variations of the resonant frequencies of the cantilevers before and after the reaction to carry out a quantitative analysis of the bio-element included in the sample.

Abstract translation: 本发明公开了一种用于测量生物元素的系统和方法，其能够准确检测在具有受控温度和湿度的气氛或气相中是否存在诸如蛋白质，基因等的生物元件，并测量生物元素的含量， 元件。 根据本发明的实施例，该方法包括制备具有多个悬臂的悬臂传感器的步骤; 测量所述多个悬臂的基本共振频率; 使悬臂与包括生物元件的样品反应; 在与外部环境隔离的封闭系统中测量反应后的悬臂的谐振频率，并将其温度和湿度控制在特定状态; 并计算反应前后悬臂的共振频率的变化，以对样品中包含的生物元素进行定量分析。

公开(公告)号：EP2353002A1

公开(公告)日：2011-08-10

申请号：EP08878142.2

申请日：2008-11-11

Applicant: KIST Korea Institute of Science and Technology

Inventor： HWANG, Kyo Seon ,  KIM, Tae Song ,  KIM, Sang Kyung ,  LEE, Sang Myung ,  LEE, Yoon Sik

IPC: G01N33/53

CPC classification number: G01N33/54373

Abstract: Provided is a protein detection system using a micro-cantilever and based on immune responses, wherein the micro-cantilever shows significantly improved sensitivity to allow detection of a trace amount of biomolecule. To the micro-cantilever, sandwich immunoassay is applied, and the sandwich immunoassay uses a polyclonal antibody or silica nanoparticles having a monoclonal antibody bound thereto, so that variations in the output signals of the cantilever are amplified and the detection sensitivity is significantly improved. The system enables detection of disease specific antigen at several femtomolar levels, and makes it possible to detect a trace amount of protein related to diseases, particularly to cancers, with ease.

公开(公告)号：EP1789779A1

公开(公告)日：2007-05-30

申请号：EP05808854.3

申请日：2005-09-15

Applicant: Korea Institute of Science and Technology

Inventor： LEE, Jeong Hoon ,  KIM, Tae Song ,  YOON, Dae Sung ,  HWANG, Kyo Seon

IPC: G01N29/12

CPC classification number: G01N29/022 ,  B82Y30/00 ,  B82Y35/00 ,  G01N29/036 ,  G01N29/326 ,  G01N2291/0215 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0258 ,  G01N2291/0426 ,  G01N2291/0427 ,  Y10T436/143333

Abstract: Disclosed are system and method for measuring a bio-element capable of accurately detecting whether a bio-element such as protein, gene and the like is present in an atmosphere or vapor phase having controlled temperature and humidity thereof and measuring a content of the bio- element. According to an embodiment of the invention, the method comprises steps of preparing a cantilever sensor having a plurality of cantilevers; measuring a basis resonant frequency for the plurality of cantilevers; reacting the cantilevers with a sample including a bio-element; measuring resonant frequencies of the cantilevers after the reaction, in a closed system that is isolated from an exterior environment and temperature and humidity thereof are controlled to a specific state; and calculating variations of the resonant frequencies of the cantilevers before and after the reaction to carry out a quantitative analysis of the bio-element included in the sample.